- The Chipettes singing "My Mother" FULL STOP. And this is before they were adopted. It's a shocking reminder that they don't often have in the show. All of the main characters are orphans.
- The baby penguin needing his mother badly. He would have died if Brittany hadn't changed her mind.
- The Chipettes discovering that the entire race was a trick; you can just see the fear on Brittany's face when she realizes that their friends are still out there and in danger.
- Theodore about to be sacrificed. Alvin and Theodore unsuccessfully try to rescue him, and only out of sheer luck do the Chipettes save them.
- Alvin's hat falls off as the kids are being kidnapped. Cue Alvin screaming for help and Dave's Adult Fear.Alvin: Dave! HELP!(The car carrying Alvin drives off as Dave runs out onto the road horrified)Dave: Somebody! Help! My son's in trouble!
Tear Jerker / The Chipmunk Adventure