John finding a photo of him and his wife in the library.
John being forced to give away his wedding ring after cutting off his finger.
It's kinda hard not to feel bad for Zero when he dies, if only because of how much a fan he was of John's. Not only that, but he has a pet cat at home, who is now alone and without an owner.
When John mentions Sofia's daughter, the latter's bitter hostility melts away and just for an instant we the person underneath - a sad, lonely woman who can never again be with the one person she loves the most.