- Aeryn's temporary death in the season 2 finale.
John: Go away, go away Zhaan. There's no point.
- John's complete despair afterwards, to the point that he doesn't even care that his brain is destroyed and he's trapped forever in his own mind
- When Zhaan goes into his head, we hear a lot of sinister whispering from Scorpius, and then Johns voice, loud and clear, telling Zhaan that its pointless to try helping him. Her response was to help him by performing assisted suicide. The others stop her thankfully, but Zhaan would never take an action like that lightly, so his depression must have been unimaginable.
- "Officer Aeryn Sun, Special Peacekeeper Commando, Icarion Company, Pleisar Regiment. Have you come to reassign me?"
- The hanger scene in the season 3 finale
- Hell, the whole last half of 'Dog With Two Bones'. Grab tissues, cuz you're gonna need 'em. John's last fantasy flash is less horrific to a post-Red Wedding audience but still shocking and pretty frelling sad. That scene got the waterworks going, and the hanger scene made sure they kept on coming.
- The general circumstance of the season 3 finale and the season 4 opener- John has been completely abandoned by all his friends, and by the woman he loves. He's alone on a dying leviathan with no real hope of escape.
- Zhaan in 'Dream a Little Dream of Me'. After over a year of freedom, she finds herself imprisoned once more. She spends the whole episode justifiably petrified.
- Her conversation with the hallucination of Crichton, telling him how much she needs him and the others, that they are her strength and without them she's hopelessly lost
- The circumstances of "...Different Destinations". Seeing through the eye of the viewfinder and watching the nurses scream out for Crichton in terror while being massacred. A real downer ending if ever there was one.
- The final scene shows D'Argo finding a marker on the wall and looking at it sadly. It was made by the child he befriended, Cyntrina, who worried no one would remember her if she died.
- Anything involving Talyn where Moya is desperately trying to get her kid to listen.
- Moya being willing to sacrifice herself for her occupants.
- Zhaan's death. It's one for both the main characters and the audience. And the look of complete heartbreak on Stark's face is enough to make you cry.
- Talyn and Crais's sacrifice at the end of season 3, where they blow themselves up to save everyone, Crais waking Talyn up for just long enough for them to die together counts, especially considering the lead-up with Talyn trying and failing to control his psychological problems to the point where he attacks his own mother.
- Gilina's death. Especially her asking Crichton, "If things had been different, could you have loved me?" "Yes."
- Most of the backstories for the main characters contain this as well.
- Crais wants revenge on Crichton for the death of his brother, because the pair were conscripted into the Peacekeeper forces, and the last words his father said to Crais was to take care of his brother.
- D'Argo's family, due to he and his wife being Star-Crossed Lovers due to her being a Sebacean, who was murdered by her brother for marrying a Luxan, and D'Argo was subsequently framed for her death.
- The death of Talyn!Crichton. Especially Aeryn bursting into tears and curing up next to his body as the screen fades to black
- The Villain of the Week in "Twice Shy" has the ability to take away the crew's most notable traits. Crichton loses his Determinator status and becomes resigned to imminent death.Crichton: [to Aeryn] You know what the worst part of this is? You and me. We never could get it together. Now we never will.
- "DNA Mad Scientist" really puts Aeryn's place into perspective. D'Argo, Zhaan, and Rygel have a chance to go home. Aeryn is also certain that Crichton will eventually find a way back to Earth, too. An attempt to find a place of her own to live leaves her being experimented on by Namtar. After revealing the increasing Body Horror to Crichton, she says, "I didn't want to get left behind. I'm so scared."
- Season 4's 'Constellation of Doubt'... because watching Crichton losing his faith in humanity due to that frellnik documentary, plus how the camera pans over to Chiana hearing the unkind things that the human commentators had to say about her... yeah. And if that isn't enough of a downer, pretty much everyone on the crew is convinced that Aeryn is as good as dead, and even if she isn't, they have no way of finding her and Crichton is the only one who even thinks its possible, is absolutely heart-breaking.
- Scorpius' memory of his first twelve years of life in "Incubator". While it in no way justifies the crimes he's committed, you can't help but feel sorry for the child he once was.
- The final moments of "Bad Timing". John and Aeryn have just got engaged and for a second it looks like the season is going to end happily for once...and then a ship flies overhead, its pilot identifying the cast as intruders on the planet. Unable to return to Moya, John and Aeryn embrace and kiss as the ship opens fire, its weapon reducing them to millions of crystals spread out across their rowboat. D'Argo, Chiana and Rygel can do nothing but watch helplessly from Moya's command centre. D'Argo's howl as his friends are crystallised is absolutely gut-wrenching.
Tear Jerker / Farscape