- The Partners statue in the Magic Kingdom - the one with Walt and Mickey holding hands. Especially for those who still see Walt as Mickey's father.
- On the Kilimanjaro Safari ride at Animal Kingdom you chase down poachers that have captured Little Red, a baby elephant, after shooting Big Red, the mother. Word of God says she survives, but the idea of the baby elephant, who can't survive away from Mommy, being forced away like that...
- According to Disney insider Jim Hill, in the original script Big Red didn't make it, and the riders saw her (simulated, of course) corpse in the climactic stretch of the ride. This drove so many kids to tears that it ruined the whole ride, so the script was changed and the prop removed before it formally opened. So it could have been a lot worse.
- The "Two Brothers" sequence from The American Adventure at Epcot and the current version of Great Moments with Mr. Lincoln at Disneyland. Depicting, as per the title, two brothers living at the time of the American Civil War who turn against each other when one decides to fight for the Union, and the other decides to fight for the Confederacy. Only one of them comes home alive...
- Also from The American Adventure, the "Golden Dream" sequence.
- The end of Fantasmic! Just seeing Mickey steering the Mark Twain and knowing that, in Disneyland, good always conquers evil...
- The finales of Spectromagic and Wishes. They're just such a wonderful sight to see and it stays true to the Disney magic. Illuminations: Reflections of Earth always does it too during the We Go On finale.
- "So Close" from Enchanted already sounds melancholy by itself. The part of Beauty and the Beast when Belle says, "I love you", to a dying Beast feels wrenching enough in its original context. A scene in World of Color manages to bring you even closer to crying by putting them together!
- And on top of that, it comes right on the heels of the scene showing Mufasa's death in The Lion King. Playing "So Close" while showing Simba try to wake up his dead father is just Disney taking your heart and shattering it into a million pieces.
Tear Jerker / Disney Theme Parks