Watching Vincent get ordered to cut ties with his father while being unable to respond defensively due to being Brainwashed and Crazy via the Blood Bond.
Episode 5: Fellas
Alan finally confronts Michael about the fact he's a vampire and convinces him of the truth. He breaks down into tears and confesses all he wants is a life with him, playing the piano, plus playing with their dogs. The heartbreak in his voice is palpable.
Episode 6: Flyboys and Crooks
The absolute grief that Aida's parents display while visiting her in the hospital.