- Barbatus' death."And then he just died in my arms, right there. I don't think he ever once in his life made his own choice..."
- The entire aftermath of the termite battle after which the aforementioned Barbatus is killed. Just to put it in perspective, except for Z, both sides were completely wiped out.
- Chip is (understandably) inconsolable after Muffy is killed by a flyswatter.
- A small one but after the whole bar fiasco Z has found someone he actually loves and starts acting much more lively around everyone. And then it becomes sad once again once he goes back to the bar and waits for Princess Bala, once she doesn't come around Z is now the only patron left in the bar. The bartender asks if he wants another aphid beer, to which Z sadly declines and says he's leaving for the night. It's Woody's delivery that really makes it sad.
- Later, Bala tells him she only picked him because he was the most pathetic bug in the joint. Briefly, Z's reacts with a pained expression, before covering and acting cool about it.
Tear Jerker / Antz