- The premise: our main character becomes Trapped in Another World, separated from her family and friends. While she's come to be perfectly safe, albeit after spending days in the wilderness hiding from wildlife, her loved ones don't know what happened to her: all they know is that she went out one day with her best friends and never came back. And, as we learn in "Best Fronds", none of them did.
Anne or Beast
- Anne at the end of the pilot tries to make the music box that transported her to Amphibia work again, so she can go home. Nothing happens. She opens and closes it a few times. Anne sighs, and resigns herself to being stuck in the strange village.
- Sprig apparently has had issues making friends with the other frog children in the village.
- The very unsubtle hints that her 'friends' back in the human world are anything but. While later episodes will show that they do care about her on some level, this episode makes it very clear that they mostly use her for their own benefit. This is highlighted in her conversation with Sprig at the lake in "Best Fronds," where Anne riddles off things that she believes friends do for each other such as getting things they really want regardless of circumstance, or giving a friend your new shoes if she likes them. Even Sprig, who confides earlier in the episode to have never had a close friend before, is clearly taken aback and saddened by Anne's words and all that they imply.
- The Reveal that her two girlfriends also got trapped in Amphibia; one is a prisoner at the castle with the Big Bad, and the other is missing. Suddenly the Adult Fear has gone much higher that three girls went missing on Earth.
- When Anne breaks Hop Pop's favorite cane just after he threatens to throw her out if she continues to disrespect him, she freaks out and says that she cannot go back to living in a cave. The scene then flashes back to show us exactly what that was like for her. While it manages to also be funny due to how over the top it was, it reinforces just how bad Anne's situation was before the Plantars took her in.
Flood, Sweat and Tears
- Anne and Sprig progressively finding it harder and harder to be roommates can hit close to home to anyone who's ever dealt with such a scenario. It comes to a head when Anne accidentally breaks Sprig's favorite action figure. Not even using the correct term "action figure" gets him out of his Heroic BSoD.
- Both Anne and Sprig finally confessing how hard it's been being roommates, even believing for a moment that their friendship is broken because of it.
The Domino Effect
- Anne misses her cat Domino so much that she adopts a caterpillar that looks like Domino, only for it to turn out to be a dangerous amphibian-eating moth monster.
- Just the sad way Anne looks back on her time with Domino II before she has to lure her away for good with the cat toy.
- Later, when Sprig offers a doll replica of Domino II, he realizes it may be a little homely. ...Only for Anne to tearfully embrace it and weep that she loves it. Now she misses both Domino I and II.
- Anne starts to have a nervous breakdown when her phone loses its charge, even saying that all her memories of home are gone.
Anne Theft Auto
- In-Universe when Anne reads Bessie's personal history about how she saved Hop Pop from a near-death experience one bitter winter. Anyone who's read a poignant part from a book will know the feeling very much.
- Anne makes an What Have I Done? face and covers her mouth after she calls Polly "a disgusting slob". Polly's expression after said comment is a punch in the gut.
- Having spent the whole episode trying to "fix" Polly by doing more feminine activities, the Plantar family ends up needing her to break a spitting record to win a trophy to pay off their debts. However, Polly misses her first two shots, and when asked how come she's not performing her best she says it's "hard to concentrate when you're feeling gross". Even if she openly said she found Anne's activities boring, the idea of being a girl the 'wrong' way still bit into her self-esteem a bit, which really is kind of Truth in Television when it comes to pressure on behavior. Thankfully Anne quickly realizes she was in the wrong and assures Polly that she's great either way.
Anne Vs. Wild
- Anne has a brief Hope Spot at the end where she tells the Plantars about the box, and maybe one of them knows how to get her home. Hop Pop doesn't have any answers, and Anne sadly says it was worth a try. Sprig promises they'll get her home, one way or another.
- And then Hop Pop is shown finding an entry about the box in a book. It's apparently called "The Calamity Box."
- Anne thinking that the Plantars caught a rare and fatal condition called Red leg (in reality it was the result of a mushroom that turned their skins red), and breaking down after being unable to cure them is crushing, particularly since they were the only ones who cared for her and took her in when she arrived in Wartwood.
- What's worse: The guilt. Anne feels that if she hadn't faked being sick, then the Plantars wouldn't have all gotten sick from working in the rain. Every time they bring up that she's sick and laud her for taking care of them despite it, all it does is make Anne feel worse because she doesn't deserve the praise she's receiving for her deception.
- After taming the pumpkin monster and earning its trust, Sprig betrays it and kicks it back to its cage. It's hard to not feel sorry for the poor beast.
Plantar's Last Stand
- Hop Pop losing his prized produce stand, which had been passed down for generations.
- In the duration of the episode, he had turned to manipulation in order to make enough money to save it. Just when he thinks his trickery is in the bag, in comes Mrs. Croaker, whom had been a loyal customer to the Plantar family ever since Hop Pop was a boy. Had Hop Pop's conscious not been with him, he very well could have ended up swindling someone he considered practically family.
- In the result of admitting his lies, not only does Hop Pop lose the stand, he loses the trust of the citizens, including Mrs. Croaker.
- Even after being in Wartwood for a month, Anne still gets treated as an outcast by the villagers. Being laughed at and called a 'scarecrow', Anne tries her best to toughen up during these matters. It only results in how deeply hurt she is by all this even though she doesn't entirely show it. It's the equivalent of someone at school trying to fit in only to be seen as an oddball by other students.
- While it may be seen somewhat as Laser-Guided Karma for how they've treated Anne, you can't help but feel sorry for these villagers whose valuables were confiscated, just after being accused of not paying taxes despite that they denied such.
- One can get a bit misty-eyed when Anne, even after how badly treated she was, sneaks in at least one item back to each of the citizens. The look of gratitude each of them give her is not lost either, especially Leopold Loggle who expresses happy tears when Anne manages to sneak him one of his prized wood valuables.
- Just the sheer fact that the toads nearly took Bessie away. The look of sheer horror and devastation in Hop Pop's eyes can break your heart. Gets even more emotionally unnerving when you remember his past with Bessie in "Anne Theft Auto".
- Just when Anne thinks she's done for and the toads are going to kill her, suddenly all the citizens rise up in her defense. Keep in mind that these are the same people who had treated her poorly. All it took was Anne's kindness and compassion towards the citizens to make them realize that she was not what they thought her as. One can get very emotional during this moment, especially after witnessing what Anne went through with these people in previous episodes.
Mrs. Croaker: "If you got a problem with her, you got a problem with ALL of us!
- The fact the citizens, having been terrified of the toads earlier, stood up and were willing to fight them and protect Anne...it's all just too much and would make one very emotional that these people finally view Anne as one of their own.
- Admit it, when Anne teared up over the realization that the town now respected her (albeit not under the circumstances she had hoped for)...you teared up too.
- Sasha tells Grime that if he compliments and encourages his troops instead of yelling at them, they'll do whatever he wants. Grime asks if it really works, and Sasha replies with a smirk "Oh, it works. Trust me, I have experience." Another reminder that Sasha really wasn't a good friend to Anne.
- Every Hibernation Day, someone in the town disappears without a trace, never to be seen again. It happens so often that everyone says goodbye to each other. The citizens embracing one another in sorrow is enough to break your heart, among them being a tearful Felicia Sundew hugging and kissing her daughter Ivy.
Wally and Anne
- Wally feels genuinely hurt after Anne tells him that without proof of the Moss Man, the citizens would view her just as insane as him. Anne is quick to realize this mistake, especially since she had bonded with him during the journey, and taking his advice to heart about how one shouldn't be bothered by others judgment. Thankfully, they make up just as quickly.
- When Anne thinks she's lost the music box forever, she looks like she's about to have a breakdown.
- Hop-Pop buries the music box in the ground, saying it's too dangerous, despite knowing he's trapping Anne in Amphibia forever.
- Also, think of it from Hop's perspective—the somber tone in his voice as he's burying the box clearly indicates there's something he doesn't want to talk about...
- Anne's "friends" sent her to do breakups for them. This is another example that Anne was never their friend and more like an indentured servant.