- "Wait! You forgot your propeller!"
- "I guess I took a wrong turn at Tahiti."
- Mr. Funny is heavily based on Harpo Marx of the Marx Brothers, to the point he only uses a horn to communicate.
- Mr. Scatterbrain's voice is heavily based on the late Ed Wynn.
- Nods to Frank Nelson were made here.
- Mr. Fussy's American voice is based on it.
- Mr. Scatterbrain says "Yes?" in Mall in a similar manner.
- R2-D2 and Robby the Robot can be seen in several episodes, such as Mr. Tickle's robot shop in "Mall".
- Optimus Prime shows up in the same episode, and is one of the toys Miss Helpful puts in Mr. Quiet's treehouse in "Trees".
- The opening of Mr. Messy's Space Junk is a reference to "2001: A Space Odyssey".
- Mr. Bump hanging from a clock tower in Clocks.
- The promo for the second season is based after Reservoir Dogs.
- Little Miss Calamity was based on Olive Oyl as stated in this interview.
- Little Miss Daredevil likewise was based on Evel Kineval.
- The voice Cheryl Chase uses for Little Miss Bossy is the same one she used for an equally bossy cartoon character.
Shout Out / The Mr. Men Show