- Its title is of course a homage to Ms. Pac-Man.
- Ms. Splosion Man's non-sequiturs. Examples of her babbling include:"These boots are made for 'splodin, and that's just what they'll do, 'cause one-a-these-days, these boots are 'gunna 'splode all over you!" (lyrics from the Nancy Sinatra song)"What a man, what a man, what a mighty good man!" (lyrics from a Salt 'N' Pepa song)"I'm just a girl, I'm just a girl, in the world!". Also, from Gwen Stefani: "I ain't no holla back girl!""A diva is a female version of a hustler!". Also: "Better put a ring on it!" Also: "All the single ladies, all the single ladies..." (which can be misheard as "All the glitz..." or "All the cigarettes...")"Girls just wanna have fu-un!" (lyrics from a Cyndi Lauper song)"Hit me, baby, one more time!". Also: "I'm a slave, for you!""I must break you." (Rocky IV quote)"Why don't you come up and see me sometime, big boy?" (a line closely resembling one said in the film "She Done Him Wrong")"I'm the king of the world!". Also: "I'll never let you go, Jeff, I promise!""Marlon Brando, Jimmy Dean, on the cover of a magazine!". Also: "Rita Hayworth gave good face." Also: "Like a virgin!" Also: "Strike a pose, there's nothing to it.""My milkshake brings all the boys to the yard!" (lyrics from a Kelis song)"I wave my hair back and forth! I wave my hair back and forth!" (lyrics from a Willow Smith song)"You complete me." (This could either be a reference to Jerry MacGuire or a song by Keyshia Cole)"I'll get him hot, show him what I got!" Also: "My my my my poker face."
- Ms. Splosion Man's non-sequiturs. Examples of her babbling include:
- One cutscene where Ms. 'Splosion Man reveals herself in a disguise brings to mind the original Total Recall (1990), down to the disguise's head laughing before it explodes.
- And before the Bionic Bommando boss fight, she arrives in a Johnnycab from the same movie.
- In the first boss battle alone:
- The Boss (Mighty Eternal) tells Ms. Splosion Man "I'm your boyfriend now!".
- One of Ms. Splosion Man's idle dances is the Carlton.
- During the final boss battle (which in itself is a shout out to Punch-Out!!), Ms. Splosion Man says:
- In the third level of the game, we hear a random flying car driver humming the theme to Dallas.
- The game references the Portal reference from the first game, only this time, the cake is not a lie - it's a trap.
Shout Out / Ms. 'Splosion Man