References to The Pirates of Penzance are made by, among others:
- Big Finish Doctor Who has a story called Doctor Who and the Pirates which turns into a piratical musical. In addition to using many tropes common to Musicals in general, including several lampshades about characters' tendencies to break into song. The story even gives the Doctor the song, "I Am The Very Model of a Gallifreyan Buccaneer".
- Kate & Leopold: Leopold gets the plot wrong.note Even so, that's remarkably good going considering Leopold is from 1876 and the first performance of The Pirates of Penzance was in 1879.
- Isaac Asimov's "Runaround": The proximity to selenium has disturbed Speedy's sensitive computer brain, causing him to speak whimsically, such as quoting from several Gilbert and Sullivan plays.
- The West Wing episode "And It's Surely To Their Credit" has a Running Gag about the White House Counsel, Lionel Tribbey, mistakenly attributing "He Is An Englishman" to The Pirates of Penzance (it's actually from H.M.S. Pinafore). Nearly everyone in the cast points out his mistake and that all of Gilbert and Sullivan's work is about duty.
- Aaron Sorkin also used a parody of The Major-General's Song in Studio 60 on the Sunset Strip. Unfortunately, it wasn't very impressive as there were no real jokes and it was also sung way too slowly.
- Frasier, episode "Fathers and Sons": Frasier, Niles, and Leland are familiar with, among other Gilbert and Sullivan works, The Pirates of Penzance. Martin—not so much.Leland (singing):...With many cheerful facts about the square of the hypotenuse!
Frasier, Niles, and Leland (harmonizing): With many cheerful facts about the square of the hypotenuse, with many cheerful facts about the square of the hypotenuse, with many cheerful facts about the square of the hypote-potenuse!
Martin (attempting to join in): With many awful facts about the scary hippopotamus!
- Babylon 5: Marcus Cole attempts to cheer up Dr. Franklin by singing The Major-General's song, after the episode's adventures leaves the two of them stuck in the cargo hold of a long-haul freighter. The doctor is less than amused.
- Every Major General Song ever.
- Tom Lehrer's song The Elements uses the tune from The Major-General's song.
- Thoroughly Modern Millie sets "The Speed Test" to music based on "My Eyes Are Fully Open"note . The letter being dictated actually begins "My eyes are fully open to my awful situation", and the song gets progressively faster as in the Penzance and Ruddigore songs do.
- From Hamilton, the song "Right Hand Man" has the lyric "Now I'm the model of a modern major-general..."
- Runescape has a song titled "The Pirates of Penance"
- Mass Effect 2: Dr. Mordin Solus was at some point part of the cast of a multi-species production. Mordin was apparently the designated patter singer.
- He even changed some of the Major General's lyrics to fit his current career.
- Animaniacs: Though the play itself isn't directly referenced, a few of the music numbers from The Pirates of Penzance get some Weird Al-esque treatment from the Warner Brothers (and Warner Sister) in the pirate-themed Musical Episode "H.M.S. Yakko". For instance, the melody of "With Catlike Tread" gets used for "We Surf The Seas", and the melody of "I Am the Very Model of a Modern Major General" gets used for "I Am the Very Model of a Cartoon Individual".