Chariots of Fire: Harold becomes attracted to Sybil after seeing her as one of the performers of "Three Little Maids From School are We".
The Producers: One of the actor/singers auditioning for the Show Within a Show "Springtime for Hitler" performs "A Wandering Minstrel, I" until he's cut off.
Topsy-Turvy is all about the creation of the show, as well as how it gets rehearsed and performed.
In Whoever Slew Auntie Roo?, Aunt Roo sings and dances to "Willow, tit-willow" for the party guests.
The 13 Clocks: Xingu, a traveling minstrel, describes himself as Nanki-Poo does: "a thing of threads and patches". Like Nanki-Poo, he's also royalty in disguise.
In one episode of Blackadder Goes Forth Blackadder tries to listen to a recording of The Mikado (specifically, the song "A Wandering Minstrel, I"). It isn't going well because his location is being bombed.
Frasier: During a radio prank from Bulldog, Frasier Crane admits that he acted in an all-made production of the show when he was at Oxford, noting that "people still ask to see my Yum-Yum". He then sings "Three Little Maids", much to his embarrassment when he finds out he was being broadcast on the radio, which gives his dad Martin a good laugh.
Law & Order: In the fourth season episode "Apocrypha", Profaci refers to Hendricks (leader of a religious group) as "the Grand Poobah".
The Muppet Show: On a first season episode (hosted by Valerie Harper), Rowlf and (a reluctant) Sam perform "Tit-Willow".
The Eastern Kingdom of Mikado is an important location in Shin Megami Tensei IV. In a reversal of The Mikado being superficially set in Japan to satirize British culture, the Eastern Kingdom of Mikado is superficially Medieval European, but is located in the far future ruins of Japan, is populated with the descendants of Japanese citizens, and anglicizes Japanese names (i.e. the founder King Aquila was named Akira, Kiccigiorgi Forest is named after Tokyo suburb Kichijoji).
Alvin and the Chipmunks: The titular trio perform "Three Little Maids" in a Japanese kabuki theater.
Animaniacs: The Warner Brothers and their sister Dot perform "Three Little Maids" as an audition to Mr. Director in "Hello, Nice Warners".
Family Guy: The extended DVD version of the episode "Lois Kills Stewie" features its own version of "I've Got a Little List", where Stewie takes jabs at everyone from Shakira's lyricist to the cast of Entourage.
Private Snafu: In The Goldbrick, the goldbrick fairy's song is sung to the tune of "Tit Willow".
The Simpsons: "Three Little Maids" is sung by the Simpson family in "Cape Feare" (which serves as a Foreshadowing of Sideshow Bob's performance of Pinafore later in the episode) and by Milhouse and Bart in "The Bob Next Door" as an attempt to prove the Simpsons' next door neighbor is Sideshow Bob.
