- "Sassy the Wonder Dog" is a series of radio ads from The Shelter Pet Project, where Timmy and Sassy often pause from helping one of Timmy's luckless friends out of the well in order to tell the listeners about adopting a dog from local animal shelters.
- Parodied with Laddie the Wonder Dog in the Discworld novel Moving Pictures.
- It's Garry Shandling's Show did a parody, involving a dog named Laffie.
- In Living Color! did a parody, involving a pit bull.
- On The Jimmy Dean Show in the 1960s, Dean's co-star Rowlf the Dog several times mentioned how much he admired Lassie, and Lassie made two guest appearances on the show. In the episode with Lassie's second guest appearance, Rowlf did a skit in which he acted out a Lassie-like scenario narrated by Dean, heroically fighting through hostile terrain, swimming across a raging river, and racing up the other bank to stop the criminals about to dynamite the bridge! ...at which point, Rowlf stopped the skit and complained that it would have been nice if somebody had mentioned the bridge before he swam across the raging river.
- Rowlf's admiration for the famous collie resurfaced in the soundtrack of The Muppet Movie when he and Kermit sang the duet "I Hope That Something Better Comes Along." Rowlf sings, "Or a collie that's classy ... a lad needs a Lassie ..." note
- Two in Whose Line Is It Anyway?: one suggestion for the everpopular Scenes From A Hat game was 'what Lassie is really trying to say', and later on an actual Lassie became the only animal guest on the show.
- Spoofed in the "Mindy and Buttons" segments of Animaniacs. Its Title Sequence was a direct parody of the Lassie opening.
- The Grim Adventures of Billy & Mandy has Billy addicted to a heroic Labrador also named Laddie; Billy often wonders aloud, "How many times can that kid fall down that well?" PS, he keeps watching the same video over and over.
- Martha Speaks features the in-show TV program "Courageous Collie Carlos", of whom Martha is a major fan.
- In Phineas and Ferb:
- "Oh, There You Are Perry": The Flynn-Fletcher kids find themselves in the position of Lassie's family in Lassie Come Home.
- "Belly of the Beast": According to the Captain, the shark has, among other things, tossed poor Timmy down a well.
- Tiny Toon Adventures:
- In "Hare Raising Night", Buster tricks Babs, Plucky, and Hamton into helping him rescue captive animals from Dr. Gene Splicer by telling them they're going to the Emmy awards. Plucky continues to believe this for the rest of the episode, and as he wonders what award he'll win, he gives us this line;Plucky: Gee, I wonder which award I'll get first! Comedy? (Hits himself in the face with a pie) Mmmm! Banana cream! Yuk yuk yuk yuk yuk yuk! Or drama? (Begs on his knees and cries) Please! Don't take Lassie away from the children! Or, a combination of the two; Dramedy! (Begs on his knees and cries) Please, don't make Lassie into... a pie! (Hits himself in the face with a pie again)
- In "Rock N' Roar", when Buster reunites with his pet dinosaur, Rover, he briefly pauses from his Meadow Run and asks the viewers, "Does this feel like an old Lassie episode to you?".
- In "ACME Cable TV", there is a sketch called "Flassie", wherein a talking Flassie tries to warn Elmyra that Montana Max has fallen off a cliff and needs assistance. Elmyra merely barks at Flassie.
- In "Hare Raising Night", Buster tricks Babs, Plucky, and Hamton into helping him rescue captive animals from Dr. Gene Splicer by telling them they're going to the Emmy awards. Plucky continues to believe this for the rest of the episode, and as he wonders what award he'll win, he gives us this line;
- According to the director's commentary, Where the Dead Go to Die was originally conceptualised as a Lassie parody, and the first segment still, is, while the others have other stories. Suffice to say, this is a very dark Black Comedy example, since the Lassie analogue, the black, red eyed labrador named Labby, is a horrific Hellhound that convinces the child protagonists to murder his parents, rips his unborn sibling out of the mother's womb, and has sex with him and the mother's corpse.