- The Merry Go Round Broke Down: It's mentioned that Jem and the Holograms used to be huge years ago, but now they work small gigs to get by. They're currently playing at a coffee shop in Toontown trying to raise money to get to Japan.
- A 2005 segment in Saturday Night Live called Barbie's Playhouse has Ken mention being friends with Jem.
- On "Guys and Dolls" from All My Children, Marcy loses her Barbie doll. The doll is actually a Jem doll.
- Nicki Minaj's verse in "Kissing Strangers" by DNCE contains the lyrics "Me? I'm Jem, and these bitches is the Holograms".
- In the music video to Madonna's "Sorry" one of the singers is wearing a Jem t-shirt.
- A 1987 issue of MAD involves "Bubbie", her band "Bubbie and the Rockheads", and their rivalry with "Jerm and the Hollowgrams".
- Splatoon 2: Octo Expansion contains a Jem reference in the name of one of its stations:
- Episode 2 of Big Hero 6: The Series stars super-villains "High Voltage". They're a mother-daughter duo with a 1980s aesthetic. They have a Villain Song and dance. Both resemble toy-line Jem most, with their blonde hair, pastel highlights, and pink star-shaped earrings.
- Rainbow Quartz, the 1980s-era fusion of Pearl and Rose Quartz, in Steven Universe is based on Danse.
- One gag in Family Guy has the mayor watching Jem.
- An off-colour Jem appears in the crowd scenes of the South Park "Imaginationland" episodes.
- Jerrica and Kimber cameo in "B.O.T." from The Transformers. They're on-model except for their color schemes.
- "The Ice Goose Cometh" episode of Mighty Mouse The New Adventures features a Jem parody called "Jim" on a poster, alongside other 1980s parodies.