- In the 2019 volume of Dial H for Hero, the second time main character Summer turns into Lo Lo Kick You the heroine now resembles Jem. "Truly outrageous" even gets turned into "Truly audacious!"
- The Merry Go Round Broke Down: It's mentioned that Jem and the Holograms used to be huge years ago, but now they work small gigs to get by. They're currently playing at a coffee shop in Toontown trying to raise money to get to Japan.
- A 2005 segment in Saturday Night Live called Barbie's Playhouse has Ken mention being friends with Jem.
- In the "Guys and Dolls" episode from Married... with Children, Marcy loses her prized Barbie doll. When Steve and Al locate a Barbie doll they believe is hers, the doll is wearing a Rock N' Curl Jem doll outfit.
- Nicki Minaj's verse in "Kissing Strangers" by DNCE contains the lyrics "Me? I'm Jem, and these bitches is the Holograms".
- In the music video to Madonna's "Sorry" one of the singers is wearing a Jem t-shirt.
- A 1987 issue of MAD involves "Bubbie", her band "Bubbie and the Rockheads", and their rivalry with "Jerm and the Hollowgrams".
- Linkara of Atop the Fourth Wall has made at least one "truly, truly outrageous" reference.
- Episode 2 of Big Hero 6: The Series stars super-villains "High Voltage". They're a mother-daughter duo with a 1980s aesthetic. They have a Villain Song and dance. Both resemble toy-line Jem most, with their blonde hair, pastel highlights, and pink star-shaped earrings.
- Rainbow Quartz, the 1980s-era fusion of Pearl and Rose Quartz, in Steven Universe is based on Danse.
- One gag in Family Guy has the mayor watching Jem.
- An off-colour Jem appears in the crowd scenes of the South Park "Imaginationland" episodes.
- Jerrica and Kimber cameo in "B.O.T." from The Transformers. They're on-model except for their color schemes.
- "The Ice Goose Cometh" episode of Mighty Mouse The New Adventures features a Jem parody called "Jim" on a poster, alongside other 1980s parodies.
- Since the three cartoons were produced by Hasbro, it was believed that Jem, The Transformers and G.I. Joe: A Real American Hero all existed in the same universe and as such, characters that resembled Jerrica, Kimber and BaNee (albeit with different colored hair) were seen in the background of some episodes.
- Robot Chicken:
- A sketch showed Jem as an overweight has-been reduced to performing at a girl's birthday party. She sings a very poor rendition of "Wheels on the Bus" and flirts with the birthday girl's father after he confesses that he used to masturbate to her as a boy.
- Another sketch had an expose of 80s stars that briefly showed Jem being caught urinating against a wall.
- One sketch spoofing Girl Code has Jem, She-Ra, the Baroness, Smurfette, Teela, Cheetara and Strawberry Shortcake discussing various topics regarding life.