Sebas Tian and Solution Epsilon have started to masquerade as a noble's spoiled daughter and her elderly butler in order to attract the attention of influential people. At the same time, it was also to bait criminals into attacking them during their journey to Re-Estize. The criminals are then be left to Shalltear who has been given the mission to capture people who know Martial Arts, and new magic knowledge undiscovered in the New World. Moreover, to gather more human bodies to create undead in order to strengthen Nazarick. Upon successfully baiting a mercenary group, Shalltear proceeds to attack their hideout. Leaving some minions outside as lookouts, she enters and slaughters all who stands in her way. Eventually, their best warrior, Brain Unglaus, comes forth to fight her Vampire Brides temporarily before facing Shalltear. However, he is no match for Shalltear and is struck by pure terror mentally upon realizing their difference in strength and he flees from her, further into the hideout. Shalltear, thinking there is no exit to escape through, lazily continues to slaughter her way through the hideout only to discover there was a hidden exit after all. She is then stopped by one of her minions, reporting that there was an unknown group approaching.
Heading out, Shalltear finds an adventurer group who has been patrolling the area and decides to slaughter them as well. The slaughter is abruptly stopped when a certain female adventurer throws a potion at Shalltear, a potion which she is all too familiar with. After hypnotizing the captured Brita, Shalltear finds out about the origin of the potion and fears that she may have unknowingly disrupted Ainz's plans, putting a huge burden on her mind. Then, she finds out that the team has left a ranger to keep watch over the place and that he should now be on his way to report about Shalltear. Agonizing over her failure to keep hidden as instructed by Ainz, she summons vampiric wolves to kill the ranger as she has no tracking skills herself. After a short while, one of her minions is killed and she quickly moves to the location where it was slain. When Shalltear arrives, she encounters a new group that she quickly notices are much stronger than the previous one. Believing that they are no match for her, she decides to simply capture them as well. This turns out to be a mistake, as one of their group's members uses a World Item that quickly starts to take over Shalltear's mind. Unable to stop it, she lashes out with a powerful attack that hits the one trying to subdue her, along with another member of the group, before everything goes dark.
Gazef Stronoff returned safely to the Royal Capital and he is welcomed back by King Ramposa III. He reports to the King and great nobles about a mysterious magic caster named Ainz Ooal Gown, who saved Carne Village, his warriors, and himself, from certain death. However, the nobles started to bear contempt towards this suspicious magic caster. After the meeting, the King apologizes to Gazef for sending him on a dangerous mission without the proper equipment for the job and wants to thank Ainz Ooal Gown for saving his most loyal and trusted aide. He assures the King that Ainz is a virtuous man and he will likely be satisfied with just those words. Gazef and the King meet Princess Renner and Climb. Renner tells her father that she wanted to discuss with him about her new idea for the benefit of the Kingdom. Gazef tells Climb that he needs to improve his swordsmanship and to be able to protect Princess Renner under any circumstances. After the two left, the King hopes that one of his daughters be free, but her other daughters will scold him for this.
Ainz returns to Nazarick after being informed that Shalltear has rebelled against him. Looking through the NPC status panel, Ainz realizes that Shalltear has been mind-controlled. Since he still doesn't know where she is, he decides to find her. He seeks the help of Albedo's sister Nigredo, who specializes in information magic, to find the whereabouts of Shalltear. After discovering the exact location, Ainz is informed by Narberal Gamma that he has been called to the Adventurer's Guild in E-Rantel. Taking the guise of Momon once again, Ainz proceeds to the meeting that consists of mythril adventurers in E-Rantel, where they discuss about the appearance of a powerful vampire. Ainz insists that he will fight against "Honyopenyoko," as he refers to Shalltear, alone, and even argues that involving others in the fight would simply increase the number of victims. Kralgra insists on accompanying him.
After the meeting, Ainz heads to the forest and eventually disposes of Kralgra. Ainz then travels to where Shalltear is, realizing that she has returned to her previous NPC behavior, that is to be on standby until someone comes too close or attacks her to which she will respond by attacking. Trying to free her from the mind control, he uses Shooting Star, which enables the use of the magic Wish Upon a Star three times without consuming experience. The attempt ended in failure and Ainz immediately understands that a World Item was used on Shalltear.
Ainz returns to Nazarick with the intent to visit the Treasury to obtain the World Items stored there. Taking along Albedo, Yuri Alpha, and CZ 2 I 28 Delta, the group meet up with Pandora's Actor, a personal creation of Ainz who is in charge of the treasury. Having hoped that Pandora would have the knowledge of the 200 World Items, he is quickly disappointed to find out that Pandora only has knowledge of the 11 in Nazarick's control. At first, Ainz is not sure about using any of the available World Items in the Treasury, but he eventually decides not to since he might need them in the future. Ainz is then informed that all the Floor Guardians have decided to attack in waves to eventually defeat Shalltear. While he knows this will certainly work, Ainz thinks that it is his responsibility to kill Shalltear and that he will do it alone. The other NP Cs are strongly against this since Ainz doesn't have much of a winning chance against Shalltear. Still, he has made his decision, and he will go on to face Shalltear alone.
After a long and earthshaking battle, Ainz eventually comes out victorious and slays Shalltear. Going back to Nazarick, Ainz brings Shalltear back to life in hopes that her death will release the control the enemy has on her mind. Thankfully, the resurrection does exactly that. However, there's a downside to it: Shalltear doesn't remember who used the World Item on her. Thinking that the enemies might target Sebas, who has been seen with Shalltear, Ainz decides to use this chance to bait the enemy to reveal themselves and orders to have Sebas' surroundings to be put under constant surveillance. When Ainz is asked about what to do with the damage inflicted to the place where Ainz and Shalltear fought, he tells Mare to leave it be as he plans to use the lie that a broken Sealing Crystal caused the destruction. He then lays out his plan to use the corpses to strengthen Nazarick's military force. Because of his plan, Albedo suggests using the corpses of the newly located Lizardmen, as there is a better chance they might create more powerful undead than using human corpses, who are fairly weak.
Back in E-Rantel, Momon and Nabe are both promoted to adamantite class adventurers as a show of gratitude for taking care of the vampire. Their daily exploits continue to amaze other adventurers, and some even consider them the best of all adamantite adventurers. When they return to the guild to report the result of one of their quests, Ainz receives a message from Nazarick. Upon hearing it, he orders the deployment of Nazarick's troops in preparation for the annihilation of the Lizardmen.