When Spider-Man discovers a vial of jackal serum at Horizon High, he and Ghost-Spider track down Jackals secret lab in an attempt to destroy the villains evil plans.
- Big Damn Heroes: Just as the Dark Goblin has Spider-Man pinned down, Harry shows up as the Hobgoblin to take him down.
- Bluff the Impostor: Anya gets Chameleon to reveal that he is not her stepsister by tricking him; Maria had gifted her a laptop, not the microscope that Anya said earlier in the fight.
- The Bus Came Back:
- Gwen's uncle, the Jackal, returns in this episode after a long absence since Season 1.
- The Chameleon also returns in this episode, impersonating Anya's stepsister Maria.
- The Chessmaster: Norman has been orchestrating everything behind the scenes, and Dr. Connors has been working for him.
- The Psycho Rangers: Deliberately invoked by Norman, who counters Spidey's friends with an Evil Counterpart team of their own relatives (although in Anya's case it's just the Chameleon pretending to be her sister). He doesn't count on Spider-Man continuing the theme by bringing in Norman's own Good Counterpart in his son Harry.
- The Reveal:
- Norman Osborn is alive, having survived the explosion in the Season 1 finale.
- Swarm is Miles's father, Jefferson Morales.
- Seldom-Seen Species: Jackal's test subjects are goblin sharks, which are a real species of shark.
- Shoo Out the Clowns: Grady disappears from the first half of the episode after he almost destroys the classroom.
- Trailers Always Spoil: The previews revealed Norman's presence in the episode before it aired.
- Well-Intentioned Extremist: Swarm says that he hates working for Norman, but he'll do what he has to do to stop Spy-D, whom he sees as bringing superhero battles to his neighborhood, and thereby threatening Miles's safety. When Miles unmasks, he has a Heel Realization and disappears.