Bi the Way: Lois, who teaches Meg how to kiss by practice kissing with her girlfriend Sarah.
Butch Lesbian: Meg disguises herself as one, prompting this hilarious reaction from Chris: "Mom, why is the Cable Guy here?".
Four other members of Sarah's club who identify as "Super Huge Mega Lesbians" are this.
Driven to Suicide: Meg's Sunshine Bear would rather commit suicide by throwing herself in front of a truck than be Meg's friend.
Ear Ache: When Stewies ear is severed from a hungry deer and no one notices, he replaces it with a Mr. Potato Head ear.
Girl-on-Girl Is Hot: When Lois practices kissing on Meg's "girlfriend" Sarah, Chris falls out of the closet trying to videotape what's going on. Later, Quagmire comes out of the closet and asks Meg and Sarah to sign some release forms.
Again towards the end, Quagmire is disappointed that there was no "girl-on-girl action" going on between Meg and Sarah and he showed up from hiding with a full video recording crew.
Lipstick Lesbian: Sarah, due to her long hair and more feminine looking appearance than Meg.
Mistaken for Gay: Meg's lab partner Sarah believes her to be gay and invites her to her special club. Meg just goes along with it for the attention until the end.
Noodle Incident: Megs teacher shows an episode of the educational science show Nova but he taped over it with a homemade sex tape.
Shout-Out: Meg watches as Chris hair and hat is placed on his head, spoofing the scene in The Empire Strikes Back in which Admiral Piett watches Darth Vaders helmet being lowered over his scarred head, as a sample of The Imperial March is played.
The flashback in which Peter swam with the bulls is a combination of the running of the bulls and synchronized swimming. They perform to Waltz of the Flowers from the ballet The Nutcracker by Tchaikovsky, which is a reference to the film Caddyshack.
The imagery in the scene where Brian sits at the bar depressed mimics the cover of Frank Sinatras album, No One Cares; Frank Sinatra Jr. happens to be performing at that bar.
The part where Mayor Adam West is singing is a parody of the song Edelweiss from the musical The Sound of Music.
The Sunshine Bear doll is based on Funshine Bear, the Care Bear.
Brian says that he has to live life when you can and live it hard, which Stewie calls the Chris Farley method. The comedian and Saturday Night Live alumnus had extensive drug abuse problems and died of an overdose, not unlike his idol, John Belushi, who was also a SNL castmember.
Stewie, Brian and Frank Sinatra Jr. make up the New Rat Pack. Frank Sinatra Sr. was in an all-star group called the Rat Pack.
When Quagmire attempts to videotape Meg and Sarah for a second time, his production crew is Mark Borchardt and Mike Schank, whose efforts to make an independent film were documented in the 1999 film American Movie.
When Stewie is left alone in the makeup room he decides to play 52 pickup, which is a card practical joke.
A recurring joke refers to Frank Sinatra Jr.s mother as actress Mia Farrow who was briefly married to Sinatra Sr. in the 1960s. His mother is actually Nancy Barbato.
Take That!: One cutaway showing John Goodman eating Thanksgiving dinner while his family starves.
Stewie decides not to buy a button-up jacket because it will make him look like actor/comedian Steve Harvey.
Brian reads a newspaper headline Thanks to treatment Magic Johnson down to one AID.