Utena: I don't remember saying I was a boy!
Kira: "You're... a girl."
Cagalli: "YOU ALREADY KNEW THAT!"
Kira: "Well... yes, but... seeing you like this, just... reminded me."
Cagalli: "Same thing, jackass!"
Lana: Well... Samus is a veteran of many impossible missions! Samus is a super-powered cyborg! Samus is the greatest space hunter in the Galactic Federation! (Samus removes her helmet) Samus is a... woman?!
Kevin: Whew! You sure are!
Link: Hooktail WAS a girl!
Flurrie: Wait, you're Hooktail's brother?
Kirby: But what if "he" is a girl too...
Flurrie: No, I'm sure this guy's a... guy...
Samus: Samus is a woman's name.
Ramirez: I'm an airman.
Tony Stark: Well, you have actually excellent bone structure there. I'm kinda having a hard time not looking at you now. Is that weird?
Officer Sanchez: Who were you looking for?
Hondo: Chris Sanchez.
Sanchez: I'm Chris Sanchez.
Hondo: You're Chris Sanchez?
"Hello Ciaphas," it said, though a vox unit on its chest. The voice sounded familiar, although I couldn't be entirely sure until a golden gauntlet rose to push back its visor. It opened, with a hiss of breaking atmospheric seals, and a well-remembered face, framed with golden hair, grinned at me, devilment dancing as always in the depthless blue eyes. "We really must stop meeting like this."
Clearly enjoying my stupedfied expression, which by this point I'm bound to admit would have done credit to Jurgen, Amberley's smile widened still further.
With all due modesty, I have to say I recovered remarkably fast under the circumstances.note
Tavi: The first night I met you, you gave me that [scar] with one of those stone knives. And I thought you were a boy.
Kitai: You are slow and stupid. We both know this. But have I ever deceived you?
- And Bradamante, taking off her shield,
Had next removed her helmet from her head.
In doing so, the golden coif, which held
Her tresses coiled and flat, she likewise shed.
They fell about her shoulders and revealed
Her unmistakeably as a young maid,
Who was as beautiful in countenance—Orlando Furioso written in 1516
Moriarty: You talked to one of my Lieutenants. He has over the years played the role repeatedly and with great conviction. More often than not he has done so to protect my identity. Other times it was because I suspected some potential clients might... struggle with my gender. As if men has a monopoly on murder.
You're gonna die, motherfucker, I take up five screens!
I'm gonna swallow you whole, and then you'll go down easy!
Then you'll be digested and converted to feces!
I'll take off your helmet, see the fear in your eyes
And your beautiful hair, and your well-toned thighs
And your pillowy lips and... wait, you're not a guy?
Samus: Is that a problem, you FUCK?!
Kraid: Uh, no, Prepare to Die!
Kraid was hesitating but Samus was set to fight
She got into a battle stance that made her butt look super-tight
Then Kraid said
Kraid: No wait, I think there has been a gigantic mistake
I actually just wanted to give you desserts I have baked!
But Samus said
Samus: What pisses me off most in this world
Is when enemies get nice when they all find out I'm a girl!
So do me a favor, and take your cakes and your pies,
And shove them so far up your ass that they end up behind your eyes!
Stop treating me like I'm a sex object,
Mother Brain's a woman and she gets respect!
Mother Brain: Yeah, Kraid, you never talk to me that way!
Kraid: Oh, gosh, I wonder if it's because you're a huge disgusting brain?
"Marth": And quite the actress, too. Honestly, I'm surprised you didn't figure it out until just now.
Yosuke: Wait, what...? Did he... Did I hear that right!?
Kanji: H-He's not a guy!?
Daigo: I'm sorry, Akira. It must have been tough for a lady like yourself...
Edge: What? His voice... it sounds like...!
Gan: A lady like yourself?!
Akira: I'm sorry I've been deceiving you. I'm not the younger brother of the big boss... I'm his younger sister...
Valbarossa: You'll abide by the proper etiquette if you're going to invite a lady.
Eshiria: Wha? You're a lady, Valbarossa?
Rintaro Okabe: ...I thought he was a guy.
Suzuha Amane: I fooled you? Guess my camouflage worked, then.
Link: Listen, if this is about that incident in '93, it was a really awkward time for me and I didn't—
Luigi: No. This bounty hunter guy tried to pick me up at the bar last night. Said he was only planetside for the evening and wanted to show me the time of my life. Couldn't even see his face under all that orange armor...
Link: Wait... Did this "guy" give you his name?
Luigi: Yeah. Began with a 'S'. Sam... or something.
Luigi: That's it! I told him I didn't swing that way...
Link: (facepalming) Oh, by Ganon's pork fetish, Luigi...
Luigi: What? What is it?
Link: Nothing. Not a gosh-darn thing. Say! You wanna come over and cry yourself to sleep on my couch for no reason whatsoever!?
"That's 'No, Ma'am'"
"No, M... Wait, you're a woman?"
Church: I never said I hated Tex, just that she was the reason we never got married.
Grif: Aha, I knew it! Only a chick could give me a headache this big!
Tex: What's the matter, never seen a girl before? How long have you guys been out here?
(Samus stares for a while)
Samus: Female. If that even matters.
Chuck: Thanks, sorry. Just wanna make sure I don't misgender anyone by accident.
Red Claw: Is that a problem?
Batman: Not at all. I'm an equal-opportunity crime-fighter.