- Mrs. Tweedy. In addition to being this movie's version of The Big H (the farm is designed like a Concentration Camp, and the pie machine is an Expy of Hitler's "shower rooms"), she's been favorable compared to Maleficent as a cold, calculating villain, carrying her plans out with chilling precision. Her presence alone commands fear in everyone on the farm, from the chickens to her own husband/accomplice, because they know just how cruel she can be if and when she feels like it.
- Case in point: her very first scene has her standing in the doorway of the farmhouse scowling, fists on her hips... while wearing a plush pink dressing gown, slippers and a shower cap. The fact that Ginger, Mr. Tweedy and the guard dogs all still cower in fear from her when she looks like this immediately establishes just how much of a threat she is.
- She quietly grins when taking Edwina to "the chop." Subtle, no, but it establishes that this isn't just a job for her. She enjoys it.
- Her coldness throughout the film makes her Villainous Breakdown into a menacing, Axe-Crazy animal all the more horrifying. By this point, she's acting purely out of vengeance and Blood Lust, swinging her hand axe around and making incoherent screeching noises in murderous rage with her hair in a nasty mess. Her look of sadistic glee when she (supposedly) cuts off Ginger's head shows just how much she's succumbed to primal anger at this point.
- Even after her defeat at the hands of the exploding pie machine, her face covered in gravy and sweat and her hair messed up, she's trembling with rage and probably would have continued her rampage had she not been crushed by the barn door.
- Mr. Tweedy, despite being a bit slower than his wife, proves he can be just as brutal when he first throws Ginger in the coal bin and barks to the other chickens "NOW LET THAT BE A LESSON TO THAT LOT 'A 'YA!"
(To Ginger, after capturing her) I've got a score to settle with you.
- When Mrs. Tweedy tells him to get a chicken to demonstrate the pie machine on, he decides It's Personal and tells her he knows "just the one."
- The Tweedy's vicious guard dogs pull no punches. They're very intimidating.
- The entire scene in the pie machine. While exciting to watch, it's clear that one false move means Ginger and Rocky are going to die. There's a reason Nick Park described it as "the Death Star of chicendom."
- When Rocky is sent down the chute with the vegetables, the passageway switches from "Veg," with the letters painted bright green, to "Meat," with letters painted blood red.
- The offscreen death of Edwina by axe in the beginning of the movie. You don't see anything but you hear the sound.
- And for good measure, we're treated to a shot of Edwina's skeleton on a plate after the Tweedys have her for dinner.
- The gut-wrenching fear of the similar scene later when a smiling Mrs. Tweedy kneels down to measure the praying-for-mercy Babs. Even when you know she's not getting taken to the chop, it's enough to see just how terrified she is that she thinks she will. We also know that there's a much worse reason she's not being taken to the chop.
- The way the movie mostly averts the Double Standard: Abuse, Female on Male between Mr. and Mrs. Tweedy. Poor Mr. Tweedy only tries to please his abusive wife.
- The "Game Over" screen from the tie-in video game. If you get caught, you are treated to a black screen with whoever caught you looking fierce. It's relatively tame by itself, but the sheer size of those Game Over Men, coupled with the loud music that plays on this screen, make it serve as a surprisingly effective Jump Scare.
Nightmare Fuel / Chicken Run