Substitute teachers in fiction have it bad. On top of having to deal with a class you are unfamiliar with, they also seem to be the butt of their jokes. Kids feel free to goof around, ignore, annoy, or even talk back to substitutes in ways they never would their main teachers. After all, it's unlikely the students'll ever see them again, so they feel just fine acting laid back around them.
This has nothing to do with that kind of teasing.
- A Porn with Plot example is the Hentai OVA Panty Flash Teacher. Machiko Natsukawa came back to her home town to become a teacher first as a substitute, but soon she finds herself being blackmailed by a student and later by his classmates, good part of them are Japanese Delinquents. After that, all goes downhill for Machiko.
- In an issue of The Amazing Spider-Man, Peter Parker, who now works as a high school teacher, substitutes for a class. One of the troublemakers immediately tries to prank him by throwing a paper ball at his head, but Peter's Spider-Sense kicks in and he catches the ball without turning his head. Peter then approaches the student and proceeds to show he knows what he did, indirectly slipping in a taunt about him being "a big fat wind", while letting him off without punishment.
- The entire plot of children's book Thirteen Ways To Sink A Sub is essentially a class of students trying to harass, gaslight, and otherwise cause a Wide-Eyed Idealist young substitute to cry. The class is divided into two teams, boys versus girls and the side that makes her cry first wins The Bet.
- The Berenstain Bears: In the Big Chapter Book The Berenstain Bears Accept No Substitutes, Too-Tall and his gang have a habit of bugging substitute teachers. When they find that Ms. Barr is going to be around for a full week (and is supposed to be a real pushover), they decide to go a step further and break her, and even rope Brother, Cousin Fred and Barry Bruin (the former two of whom try to keep things from going too far) into helping. It backfires when they find out they've been harassing a skilled martial artist who could, if she wanted, break them.
- The Winnie Years: In Twelve, Winnie's classmates give wrong names when a substitute teacher takes attendance, and consider playing other pranks on her, including taping her to her desk.
- Ned's Declassified School Survival Guide: In one episode, a student teacher begins working at the school and gets ganged upon because he's technically a student. Moze ends up teaching him to stand up for himself.
- In the first season of Roswell, a new substitute teacher (played by Julie Benz) is actually a government agent investigating the town's strange goings-on. Liz first gets suspicious when she claims a triangle adds up to 360 degrees, not 180, and corrects the error.
- One episode of the Weird Science sitcom has Gary wish that the classes at his high school were geared more towards his likes. After Lisa grants the wish, one of the classes is about how to torment a substitute teacher.
- Seven Periods With Mr Gormsby: The entire reason why Mr. Gormsby is employed is that all the Relief Teachers (what the position is called in New Zealand) were terrified of the class. Gormsby's immediate predecessor was so terrified she informed the principal she was immediately going home... to India! Gormsby was subjected to some of the same tactics... and immediately commanded the respect of the worst actors in the class room. It helped that he and his class had a mutual dislike of Steve, the guidance counselor.
- Inverted and satirised in the Key & Peele sketch "Substitute Teacher." Mr. Garvey is a black, confrontational substitute teacher who has taught over 20 years in intercity schools. Because of this, when the students correct him when he mispronounces their names (all of them dime-a-dozen stereotypical white names) while taking attendance, he acts as though they are giving him lip and flips out, ending with him sending Aaron (pronouncing it "Ay-Ay-Ron") to the principal's office for correcting him in the pronunciation of the principal's name.
- In the Small Wonder episode "My Mom, the Teacher", Jamie does all he can to humiliate his own mother when she's a substitute teacher for his class.
- Kickin' It - Jerry is revealed to be a self-proclaimed "Sub Sinker" in one episode. After he scares them all off, the school resorts to having Milton, one of his classmates, fill the role. As Milton already knows all of Jerry's tactics, he copes quite handily.
- Discussed: When Jeff Goldblum served as the Guest Host of The Late Late Show with James Corden for a night in 2019, his monologue opened with him presenting himself as a substitute teacher who can't be fooled by anyone in the audience into just ordering pizza for everybody instead of following the show's "lesson plan".
- In the Glee episode The Substitute, the New Directions attempt to prank substitute teacher Holly Holiday by buttering the club room floor and switching names. This isnt enough to fool Holly, as she slides across the buttered floor with grace. As for the name switching, it was pretty obvious in and of itself anyway.
- In the Hark! A Vagrant strip "Substitute Teacher", the teacher reacts mockingly when one of her students is introduced as "Tits Palmer", assuming the students are trying to get one over on her — but it turns out that really is Tits' name.
- The first Yamiko that Himei ever encountered in Sailor Nothing used a substitute teacher as its host. The woman was used to being casually disrespected by her student but she pretendd not to notice, even though it secretly bothered her. The Yamiko used the teacher's pent-up anger against her and made her want to attack children.
- Hey Arnold!: Though he was meant to be a permanent teacher, Mr. Simmons was subject to the usual tricks played on a substitute (such as switching names and dropping pencils) when he started teaching Arnold's class. They end up driving him away, and plan to do the same to every new/substitute teacher sent after. Then the next teacher sent is Lt. Major Goose, a Drill Sergeant Nasty that makes them want Mr. Simmons back—which they get by pulling the same tricks again.
- In one episode of King of the Hill, Peggy becomes a Spanish class substitute in the middle school where Bobby, her son, attends. The two bullies in the class immediately go out of their way to make her miserable just for the hell of it.
- Recess has this trope double subverted when the class has the substitute teacher Mr. E, whose nonchalant dodge of the first prank of the day convinces the kids not to try anything further, setting up the Sadist Teacher angle. Subverted when he turns out to assign independent study and helps the kids with their own independent interests, leaving only TJ to be unimpressed, though TJ's entire project was reminding the class that their regular teacher was still sick and that he hadn't forgotten about that over Mr. E's impressive credentials as a teacher.
- The Simpsons: On the episode "The PTA Disbands", Springfield Elementary has become such a Sucky School that the teachers go on strike and the rest of the community is forced to try to teach. Bart manages to annoy or terrorize several substitute teachers away from his class until his mother Marge arrives, annoying him to no end with her coddling and turning him into a bully magnet.
- Another Disney cartoon show, similar to Recess above, Teacher's Pet episode "No Substitutions, Please", where Scott decides to play nasty pranks on the substitute teacher Ms Dewygood, due to his canine super-sense and the fact that he is not used to different teachers.