Split Personalities are weird things. They can have completely opposing personality traits to the main personality, different skills sometimes... But perhaps one of the oddest from a logical standpoint is when a split personality is actually a different gender from the main personality and from the body they control.
How exactly they rationalize this gender incongruity can vary. Some may simply ignore it. Some may claim to be transgender. Some may actually be shocked to find themselves in the alternate body. Every mental coping mechanism will be a brain-twister, however. Sometimes may overlap with Gender Bender, especially if The Mind Is a Plaything of the Body.
Compare Super Gender-Bender for when your other gender split personality has superpowers.
Truth in Television for those who suffer from Dissociative Identity Disorder, for whom this is a very normal, routine occurrence along with selves of different races, ages and even species coexisting.
- Shiki Ryougi from Kara no Kyoukai—at least, until a car accident kills off her male personality "SHIKI". This impacted her a lot to the point that she's taken to adopting many of his behaviors and mannerisms as a way to remember him; given that SHIKI acted like any normal teenage boy despite being having a female body, this led to Shiki's shift into harder Tomboy territory.
- Also, her entire bloodline is capable of developing this if a specific ritual is performed when they're infants, and she's the heir of the family rather than her older brother because he was unable to manifest his second personality.
- Assassin from Fate/Zero is primarily male, but two of the personalities that can manifest alongside the other personas are a woman named "Asako" and a young girl with no name.
- Hato, the Wholesome Crossdresser of Genshiken is male but has a female inside voice/spirit/personality/whatever, teasing him each time he is with Madarame, much to his annoyance.
- The former spirit detective Shinobu Sensui from Yu Yu Hakusho has developed no less than 7 identities, one of which is a girl called Naru, who represents the last remnants of his innocence.
- Kotonoha Nanase from Gun×Clover has seven different personalities which protect her primary personality, who has been targeted since childhood because of a unique ability to read dead languages. Her fifth personality is a Hot-Blooded male martial artist named Itsuba.
- Invoked in Love Stage!!. The protagonist Izumi, an otaku from a prominent family of actors and music talents, is cast in a TV Drama as the main character, a rich young master with three personalities, one of which is a girl. This difficult role shows off Izumi's prodigious but heretofore unseen acting abilities, causing feelings of inadequacy in his senior co-star and boyfriend, Ryouma.
- Mari from Inside Mari has three personalities: The female "Mari", the female "Fumiko", and the male "Isao". Fumiko is her natural personality however Mari developed due to a combination of childhood traumas and her mother forcibly changing her name. Isao is the newest personality and developed due to her interest in the real Isao. The Isao personality is the main character of the manga and believes he is the 'real' Isao stuck inside Mari's body.
- Invoked in the The Legend of Zelda: Ocarina of Time manga, which explains Sheik as this. Seven years ago, Zelda allowed Impa to put her consciousness to sleep and replace it with a boy named Sheik.
- Linnéa in We Are All Pokémon Trainers shares a body with her male split personality, Ray, who was formed out of her desire for a protector from her abusive mother.
- A sub-plot in Session 9 revolves around a psychiatric patient named Mary Hobbes, who suffered from dissociative identity disorder and had three "alters" named The Princess, Billy and Simon. Billy and Simon sound very masculine and have masculine names while The Princess sounds more feminine. The film leaves it deliberately ambiguous whether this is actually a case of dissociative identity disorder or Demonic Possession.
- The main character from Doctor Jekyll and Sister Hyde is a man whose Hyde is a woman.
- In the famous twist ending from Psycho it turns out that Norman Bates' mother is long since dead, but Norman has a split personality who he thinks of as his mother. Even more creepily, we actually hear Norman talk in her voice near the end of the film.
- In Identity, the ending reveals that all of the ten main characters, consisting of seven males and three women, are alternate personalities of Malcolm Rivers.
- An in-universe example in Adaptation., in which Donald's inane psychological thriller screenplay The Thr3e ends with the reveal that the cop protagonist, the killer he is chasing after and the female victim the cop falls in love with are all the same person. Donald chooses to cheerfully ignore all of the Fridge Logic and Plot Holes created by this plot twist.
- Carter in Raising Cain has several split personalities, who are leftovers from the research he went through as a child. One of them is a quiet, intimidating woman named Margo, who takes over to make him escape the police, and go after the real villain of the story.
- In Split, at least two of Kevin's 23 personalities are women. One of them, Jade, gets very little screentime but is diabetic and needs insulin injections (which none of the others do,), while another, Patricia, is one of the main antagonists of the film and acts as an Evil Matriarch to several of the other dangerous alters. It's hinted that Patricia not only considers herself female, but may actually perceive Kevin's (physically male) body as female when she is in control; at one point, when she takes over from a male personality who had no problem with Kevin being a Walking Shirtless Scene, she immediately covers her chest.
- Glass adds a third, Felida, the personality of a Latino woman.
- The Truth introduces Altogether Andrews, a beggar with no less than 8 different personalities, one of which - named Lady Hermione - is female. They activate at random, and can apparently communicate with one another. And none of them respond to "Andrews".
- Carpe Jugulum has Nanny Ogg remember dealing with a man who had seven personalities, three of which were female. The "primary" (male) personality claimed the other six just paired off in the back of his head and left him to get on with things.
- When Rabbit Howls, the autobiography of Truddi Chase and her Troops, matter-of-factly describes both male and female selves, different ages and races. One of the powerful protective selves was Mean Joe Greene, a huge black man. Chase was physically a white woman.
- Bates Motel, as a TV prequel to Psycho, has Vera Farmiga playing both the real Norma Bates and Norman's manipulative secondary personality "Mother".
- In one episode of Criminal Minds, the Serial Killer they're looking for isn't quite their prime suspect, a man named Adam; it's actually his alter, a woman named Amanda. Despite her personality existing in Adam's male body, s/he is enough of an Attractive Bent-Gender for her to successfully seduce her (male) targets before killing them. Once she's found out as the killer, Amanda performs a Split-Personality Takeover.
- In a House episode, a girl develops an alternate personality whom she considers her "boyfriend". Oddly enough, she tests positive for pregnancy and the doctors think he got her pregnant, which doesn't turn out to be true.
- One Law & Order episode revolved around a male-bodied system with a seemingly all-male crew, but there was a single female member who, unbeknownst to the guys, protected them from the person abusing them. In another story, a female had a male self to protect her from her abuser. Again, she would black out when he showed up, so had no knowledge of what he did; both episodes hinged on how much proof of the diagnosis there was and whether they could make an Insanity Defense.
- One Life to Live. One of heroine Viki's alternate personalities was an angry teenage boy. And after she finally remembered that her father had sexually abused her from age 7 to young adulthood, he became one of her personalities too. This isn't unheard of for real multiples.
- When her younger brother Todd faked DID to get off on criminal charges, one of the personalities he created was a woman.
- In a first season episode of Shawn and Gus are hired by a client who turns out to have multiple personalities. One of them is a woman who has been trying to arrange a sex change behind the (male) primary's back, another is a murderous psychopath who kills the doctors the female alter has been going to.
- In another episode, Gus falls for an extremely attractive woman... at a mental hospital. Eventually, he learns that she is sharing a body with another personality named "Frank".
- United States of Tara is about a woman with DID named Tara Gregson and her multiple personalities, one of whom is a man named Buck who claims to have lost his "member" in 'Nam.
- Another male alter, Bryce, manifests in season three.
- In an episode of The X-Files there was a woman who claimed to have a Split Personality; one of the personalities was male.
- In killer7, KAEDE is one of the many split personalities of Harman Smith and the only female one. The appearance and physical characteristics change to match that of the personality in charge though, and it's never made totally clear how these personalities came about in the first place as the game is one big Mind Screw.
- Umineko: When They Cry has a variant of this: Kanon and Shannon are actually alter egos of Sayo Yasuda, and while they're played as a single person leading a double life rather than DID per se, the way in which the personas are used to dramatize their inner conflict borrows a lot from classic split personality tropes. While the backstory as revealed in Episode 7 leaves the precise circumstances of their upbringing with respect to gender ambiguous, some unreliable narration would suggest they were male at birth.
- Sequel series Ciconia: When They Cry has a more straightforward example when an early game twist reveals that protagonist Mitake Miyao has a little sister alter by the name of Meow. So far, this has largely been in service of a lighthearted love triangle in which a male squadmate finds himself taken with the both of them, but it's one factor in Miyao's mounting discomfort with existence as a physical being as a whole. While they're both private about the details of their sex, it can be inferred through flashbacks that they were raised as a boy.
- Uendo Toneido from Phoenix Wright: Ace Attorney Spirit of Justice suffers from multiple personality disorder. Most of his personalities are male, but one of them, Kisegawa, is a female courtesan (the game even switches to the higher-pitched text blips used for female characters when said personality takes over).
- When we get a look inside Elliot's brain in this El Goonish Shive strip, a couple of his personality facets (judging by the dialogue, his shyness and his curiosity) are female, while the rest (confusion, ego, libido, and in the last panel conscience) are male. The oddness of this is explicitly pointed out in an alternate version of the relevant panel.