A Barbershop Quartet is a four-person (traditionally all-male) band which sing A Cappella in four part harmony. Like most music they can and often get used for something other than their musical merits. Oftentimes, Barbershop quartets are used for comedic effect or as a way to deliver insults that may be too crude or outrageous to be said normally but somehow becomes much more acceptable when sung by a Barbershop Quartet.
Compare to Gratuitous Mariachi Band, another musical group that is often used for comedy.
- A popular Icelandic ad for prostate cancer awareness involves an (imaginary?) barbershop quartet singing to an old man while encouraging him to get checked for cancer symptoms. It does, indeed, make this serious subject considerably more funny.
- A DirecTV commercial features NFL star Peyton Manning (who has DirecTV) and his "really high-voiced" clone (who has cable). While the real Peyton is watching NFL games on his phone app in the comfort of the team bus, his high-voiced counterpart is singing "Camptown Races" in a school music room with his barbershop quartet, the Four Tunesmen.
Real Peyton: Don't be like this me.
- Animal Crackers (2017): At one point in Horatio's Villain Song, Horatio's henchmen come out of nowhere and repeat one of his lines in barbershop quartet style, complete with appropriate hats. Horatio angrily shoos them out, saying "No harmonization, thank you." The creators hired a real barbershop quartet just for this gag.
- The Haunted Mansion: The singing busts are a barbershop quartet of Living Statues who repeat the words other people say as a song or go off on tangents from there, making any attempt at a sensible conversation with them impossible. They were portrayed by Disneyland's own barbershop quartet, the Dapper Dans.
- The Muppets: One of the skits the Muppets do for their telethon is a barbershop quartet rendition of "Smells Like Teen Spirit", including Beaker who hums his "meeps". Inexplicably it ends with them shrinking Jack Black's head with a hot towel.
- This is Spın̈al Tap: Defied when David said that he didn't like how their singing at Elvis Presley's grave sounds like "fucking barbershop, barbershop raga." They just can't sing "Heartbreak Hotel" that well.
- Friends: In one episode Ross hires a barbershop quartet to visit Rachel's office on her first day at work and sing about how much he loves her. Rachel finds it embarrassing as it's clearly meant to remind her co-worker that she's taken. At the end of the episode Monica uses the same quartet to call out Julio, a waiter she'd just broken up with, for being a sexist, pretentious jerk.
- Last Week Tonight with John Oliver: After a two-year legal battle with coal magnate Bob Murray, John Oliver launches into a 1930's song and dance number including a barbershop quartet of seven-foot squirrels. Accusing a man of being a furry who has sex with squirrels? Strange. Having seven-foot squirrels do it in a barbershop quartet complete with period accurate costumes? Hilarious!
- Red Dwarf: In "Officer Rimmer", Rimmer gets four of his bioprinter created clones to form a barbershop quartet, complete with a Rimmer-ified version of "Mister Sandman".
- The Tonight Show Starring Jimmy Fallon: The Ragtime Gals skits, which include covers for several Intercourse with You songs. Sometimes, they even have more than four people singing.
- The Two Ronnies: One of their musical finales is a barbershop quartet set in a barber's shop, with the four barbers/musicians inflicting slapstick gags on their customers.
- The Music Man: Harold Hill organizes the four members of the River City School Board into a barbershop quartet that bursts into four-part harmony at the slightest excuse.
- The Action 52 Owns version of Fuzz Power features a quartet of literal barbers as major characters. They even sing the game's intro and ending!
- The Curse of Monkey Island: The three pirates at the Barbery Coast in Puerto Pollo (Edward van Helgen, Haggis Macmutton, and Cutthroat Bill) are looking for a fourth so they can form a barbershop quartet. Guybrush can offer to audition for Edward, driving him up the wall with some truly awful singing.
- Cyanide & Happiness has done this a couple of times, once to hit on a girl from a taxi and another time as a barbershop quartet of surgeons.
- A barbershop quartet is one of the many people to get blown up by stepping on the Mine Turtle on ASDF Movie.
- A barbershop quartet consisting of Ed, Ted, Ned and Fred were one of the characters to audition in CartoonMania.
- In Faux Pas, at the dual wedding for Randy and Cindy and Jon and Penny, a group of cats in this strip appear to offer their musical services in place of the originally planned choir. The foursome strike a classic corny barbershop quartet pose, with one of the cats even holding a straw hat! They get passed up.
- One of the The Fairly OddParents shorts on Oh Yeah! Cartoons, Timmy wishes to copy himself several times. At one point, four of his copies sing like a barbershop quartet, seemingly for no other reason than to mess with Vicky.
- One Family Guy Cutaway Gag had Peter break Bad News in a Good Way to a terminal AIDS patient by having a barbershop quartet sing about his disease.
- Another episode had them show up to sing about a vasectomy.
- When Harvey Birdman, Attorney at Law's Deadly Duplicator accidentally makes four copies of Mentok, they immediately form a barbershop quartet. Phil shoots three of them, and Mentok starts looking for replacements, starting with a baritone.
- In the Lilo & Stitch: The Series half-length episode "Mrs. Hasagawa's Cats", it's shown that Jumba made a four-headed barbershop quartet experiment, Experiment 044 (a.k.a. Forehead), who is designed to annoy people by... well...
Forehead's first three heads (singing): We're evil 'cause we sing!
Forehead's fourth head (singing badly): Annoyingly off-key! (joined by the other three heads on the last syllable)
Stitch (pulling his ears down): Yeesh!
- The Simpsons: An entire episode was dedicated to Homer's old barbershop quartet, whose story is a clear send up to The Beatles, complete with a Sgt. Pepper's Shout-Out and naming one of their albums Bigger Than Jesus.
- SpongeBob SquarePants: One episode has the prehistoric counterparts of SpongeBob, Patrick, and Squidward encounter fire for the first time. In a parody of the "Dawn of Man" sequence from 2001: A Space Odyssey, the prehistoric SpongeBob very slowly realizes the potential usefulness of the fire as the Ominous Latin Chanting of "Lux Aeterna" by Gyorgy Ligeti plays - but the ominousness turns comedic when we see that the choral piece is being sung by a live-action barbershop quartet.
- In the episode "DeeDee be Deep" of Dexter's Laboratory, DeeDee annoys Dexter by practicing her singing for a singing competition extremely loudly and very badly. Dexter gets her to let him use a machine on her to make her voice less annoying, but she messes with the controls causing her voice to become extremely deep, so deep in fact that she destroys things when she sings. At first she's sad because she doesn't win the singing competition but then she ends up joining a barbershop quartet.