Traditionally the highest valued card in many card games, the Ace of Spades has become symbolic of death or danger. Should a character find themselves drawing or otherwise recieving this card, they best start looking over their shoulders.
- One Piece: The character Portgas D. Ace used to be captain of his own pirate group, the Spade Pirates, before it was assimilated into Whitebeard Pirates. And he later becomes the first major character to die in the present.
- The anime adaptation of Kaguya-sama: Love Is War assigned all the starting members of the Student Council a Playing Card Motif. Naturally, the perpetually depressed Ishigami -who spent most of the earlier chapters afraid that Kaguya was trying to kill him- was assigned the Ace of Spades.
- The Spades are derived ultimately from the Swords of the Tarot, and the associations carry over of tarot cards to the more "mundane" playing deck. The Ace of Swords is to do with cutting loose from old ways and habits, dramatic change, et c; badly aspected it can mean violent death or serious illness or an act of violence. (Interestingly, the Nine of Swords/Spades has a worse Tarot meaning: this is the one that can mean Death, or at the least, something unlucky or unfortunate...)
- The initially cordial man-pig dinner party at the end of Animal Farm ends in chaos when during a poker game, Napoleon and Mr. Pilkington simultaneously play an ace of spades. Most critics view this as Orwell's interpretation of the Tehran Conference, ostensibly meant to establish a unified front among the allies but in actuality setting up future conflict between the Soviet Union and the West.
- Whateley Universe: Inn Silver Ghost, Golden Angel (Part 3), used by Cardsharp:
The card was the Ace of Spades, the Death Card. Cardsharp held before him, like a priest presenting a crucifix to a vampire. FEAR, mortal terror, primordial panic radiated out from the card, filling Kirk's mind with dread.
- Baccano!: The Dreaded Professional Killer Claire Stanfield is sometimes associated with the Ace of Spades in its capacity as the "death card".
- Motörhead's song "Ace of Spades":
Pushing up the ante, I know you gotta see me,Read 'em and weep, the dead man's hand again,I see it in your eyes, take one look and die,The only thing you see, you know it's gonna be,The Ace of Spades
- In act 2 of Carmen, the titular character draws spades during a fortune-telling game which she believes foretells her death. Sure enough, her former lover Don Jose kills her in a jealous rage.
- In Pinocchio, Honest John tricks Pinocchio into going to the dangerous Pleasure Island by giving him his "ticket", an Ace of Spades card.
- During the Vietnam War, US soldiers would use the ace of spades as a form of psychological warfare, since the North Vietnamese allegedly feared the card because of the superstitions surrounding it as a harbinger of death. Scattering these death cards in real or suspected hostile areas reportedly caused the Viet Cong to flee. In addition, some American troops would leave a single ace of spades card on the body of an enemy kill to indicate "We were here" or "We're coming for you."
- During the Second Gulf War, the United States distributed a deck with the 52 "most wanted" Iraqis with Saddam Hussein being the Ace of Spades.