Some of these works have characters coming out or being shown to be LGBTQIA+ as reveals, beware of spoilers.
See LGBT Representation in Media for a list of works in other mediums with prominent LGBTQIA+ representation.
See also Queer Media for works with a primary focus on LGBT people, queer subjects and themes and Queer Romance for works that focus on romantic relationships between queer characters as the main plot. See also LGBT+ Creators for a list of artists/media creators who are LGBTQIA+.
- Adventure Time: BMO is agender, and Marceline and Princess Bubblegum's sapphic relationship is a major subplot of the series.
- Adventure Time: Distant Lands: Princess Bubblegum and Marceline are queer women, and their relationship is a major part of "Obsidian".
- Adventure Time: Fionna and Cake: In the alternate Mundane Universe, the male and mundane versions of Bubblegum and Marceline, Gary Prince and Marshall Lee, also hit it off. Their budding romance is a recurring subplot of the series and serves as a bit of a relief from the darker main plot.
- Allen Gregory: Richard, Allen's dad, is a gay man whose abuse towards his partner is Played for Laughs.
- Amphibia: One of the main characters, Sasha Waybright, is bisexual, which is revealed in a Freeze-Frame Bonus in the series finale. The next two pairings aren’t given a specific orientation, but Marcy's companions in Newtopia, Yunan and Olivia, have settled down together after the Time Skip. And then in Wartwood, Mayor Toadstool and his assistant Toadie are also shown to be dating in the epilogue. Among the Earth allies of the Plantars are the I.T. Gals Ally and Jess, a sapphic couple (specifically, Ally is pan and Jess is bi). One of the Plantars' enemies on Earth is Mr. X, an Agent Peacock voiced by Ru Paul who mentions having a husband. And then amongst the civvies are two men who are neighbors in a romantic relationship.
- Archer: Out of the major cast, Pam is a bisexual woman while Ray is a gay man.
- Archibald's Next Big Thing: Jean-Pierre, a swim instructor and log roller, is gay and later gets married to another man.
- Arthur: Arthur's teacher Mr. Ratburn gets married to a man named Patrick late in the series.
- The Avatar Universe has become synonymous with introducing LGBTQIA+ characters to its world. Various works released since have retroactively introduced numerous gay characters to the franchise's parent series.
- The Legend of Korra revealed that the titular main character and her best friend/lover Asami Sato were bisexual, having dated the same man Mako in Books 1 and 2, before becoming a couple themselves at the end of Book 4, an unprecedented move for a major western cartoon aimed at children at the time and arguably led to later creators taking greater risks in introducing LGBTQIA+ themes in their work.
- Baymax!: The third episode has Baymax go on a Tampon Run with one of the people he meets being a trans man. Another episode has Baymax help Mbita work up the nerve to talk to his crush, Yukio.
- Big Mouth: Sexual and gender identity during puberty is one of the issues explored in the show from various persectives. Part of Jay's character development is realizing and accepting that he's bisexual, Matthew has several stories relating to his experience as a gay kid, and other recurring characters, such as Ali (pansexual), Natalie (transgender), and Elijah (asexual) introduce various other orientations. Maury and Connie's child Montel is also raised without gender and basically treated as nonbinary, and sings a song about defying gender roles.
- Human Resources (2022): Not only do the monsters live in a Non-Heteronormative Society, but casual LGBTQIA+ representation is even more common than in the parent series Big Mouth. For example, trans girl Natalie returns as a recurring character, and one episode focuses on her college-aged lesbian sister choosing between pursuing her dream college or following her girlfriend. Sonya also ends up in a star-crossed romance with a human woman, though the main conflict there is the Interspecies Romance angle. Season 2 also has a minor subplot about a lesbian couple raising a son together.
- Big Nate: Slice of Life animated series based on the comic strip and novels on the misadventures of mischevious sixth grader Nate Wright. Nate's friend Dee Dee is a lesbian (a change from the comics), which she realizes when she's captivated by a substitute drama teacher and then sees her with her wife.
- Bob's Burgers: A recurring character is Marshmallow, who is transgender.
- BoJack Horseman: Todd Chavez, one of the five major characters, is asexual, and part of his character arc involves coming to terms with it and navigating his relationships after coming out. Also, Hollyhock is raised by her eight adoptive gay dads, all of whom care for her well. Meanwhile, BoJack had a falling out with his friend Herb for not supporting him when Herb was fired after it was revealed that he was gay.
- Braceface: Sharon's high school mentor/fashion designer Mark "Dion" Jones is gay. One episode even sees Sharon helping him find a boyfriend.
- The Bravest Knight: Sir Cedric and Prince Andrew are gay dads raising their adopted daughter, Nia. Cedric tells her stories about how he became a knight.
- Captain Laserhawk: A Blood Dragon Remix: The title character, Dolph Laserhawk, is in a relationship with another man as the series begins.
- Castlevania (2017): Alucard is bisexual.
- Centaurworld: Zulius is gay, and has a minor arc involving a rivalry-turned-romance with another man. A few jokes also reveal Ched is attracted to men, including Zulius.
- Central Park: It's brief, but in the episode "A Decent Proposal", Shampagne, a male dog, falls in love with another male dog.
- Chibi Tiny Tales: Multiple characters have their canon selves' sexualities, such as Luz being bi and Amity being a lesbian.
- Chozen: The main character Phil Cullins/Chozen is a gay man.
- City of Ghosts: Tom, a main character, is non-binary and Jasper, another main character, has two mothers.
- Clarence: Jeff, a main character, has two mothers.
- Cleopatra in Space: Akila has two mothers in the series, Theoda and Pothina.
- Clone High: Cleopatra, one of the main cast of students, is bisexual; she had two male love interests in the original series, JFK and Abe Lincoln, and despite her initial disgust upon learning that her latest interest "John D'Arc" was actually Joan of Arc dressed as a guy, even admits with a smile that doing her would've been hot. In the 2023 revival, she starts a relationship with Frida Kahlo, who is also (presumably, based on her real life counterpart) bisexual.
- Craig of the Creek: Kelsey is confirmed to be a lesbian, which is shown through her Ship Tease with Stacks, with the two of them becoming an Official Couple in season 4, but there are several other LGBTQIA+ creek kids/supporting characters, such as Angel, who is agender, and Raj and Shawn of the Honeysuckle Rangers, who are gay. Craig has a cousin, Jasmine, who mentions having a girlfriend. JP's older sister, Laura, also has a girlfriend, and recurring characters Tabitha and Courtney are girlfriends and even kiss onscreen once.
- The Crumpets: Grownboy is bisexual and Steve is his partner and eventual husband.
- Danger & Eggs: There are numerous LGBTQIA+ supporting characters, including Milo (a nonbinary kid that the protagonists form a band with) and Zadie (a young trans girl). There is also a whole episode about a Pride Parade, and an overarching theme of chosen families.
- Dead End: Paranormal Park has a Cast Full of Gay: Barney is a gay transgender boy, Norma is bisexual, and Courtney is a non-binary demon.
- Disenchantment: The main character Bean is a bisexual woman in love with a mermaid. Also, supporting characters Odval and Sorcerio are a gay couple.
- Dodo: There are a few same-sex couples among the students and Joe's friend Frisbo has lesbian parents.
- The Dragon Prince: There are three prominent same sex couples featured in the show, which are depicted being as commonplace as hetero romances. The Moonshadow elves Runaan and his husband Ethari, who were entrusted to raise the main heroine Rayla by her parents, the Queens of Duren, who gave their lives to save their kingdom and daughter, and as of season 4, the deaf, Human General, Amaya and her enemy-to-lover, the Sunfire elf Queen, Janai, who were able to communicate with the help of non-binary Sunfire elf interpreter, Kazi. Season 4 introduces Terry, a transmasculine Earthblood elf, as the boyfriend of the dark mage Claudia.
- Dragons: The Nine Realms: Alex, one of the main leads, has two mothers.
- Drawn Together: Xandir P. Wifflebottom, is gay though all the main characters have shown queer tendencies throughout the series.
- DuckTales (2017): Webby's friend Violet Sabrewing has two dads.
- Elliott from Earth: Elliott has two mothers but only Frankie, who is also a main character, is present in his life.
- Freedom Fighters: The Ray: Ray, the main character, is a gay superhero.
- The Ghost and Molly McGee: One of Molly's teachers, Mrs. Roop, is shown to have a wife. Scratch's friend Geoff is revealed in Season 2 to have a boyfriend named Jeff. Season 2 also reveals that one of the main supporting characters, Andrea, is sapphic, as it is revealed in "Davenport's in Demise" that she has a crush on a girl named Alina.
- The Great North: One of the main characters Ham is a gay teenager who would later start a relationship with another gay teenager, Crispin.
- Gravity Falls: Recurring characters Sheriff Blubs and Deputy Durland are confirmed to be gay in the series finale.
- Hailey's On It!: Scott's sister Becker is a lesbian who previously had feelings for Hailey before dating her former rival, Kennedy. Hailey's pet flamingo Frank is also in a relationship with Petey, a male peacock.
- Harley Quinn (2019): Harley and Poison Ivy are both bisexual, while Clayface identifies as sexually fluid.
- Hazbin Hotel: Much of the main cast is queer: Charlie and Vaggie are a sapphic couple, Angel Dust is gay, and Alastor is implied to be asexual.
- High Guardian Spice: One of the main characters, Thyme, is bisexual, while one of the major recurring characters, Professor Caraway, is transgender.
- The Hollow: Adam, one of the main three characters, is gay. Another main character Mira, has two fathers.
- I, Elvis Riboldi: Emma Foster, one of the three main characters of the show, has two moms. One of whom is also the doctor of their school.
- Invincible (2021): Like in the original comic, Mark's best friend William is gay.
- Inside Job (2021): Andre is pansexual, and most of the main cast is Ambiguously Bi.
- Jurassic World: Camp Cretaceous: Yasmina and Sammy, two main cast members, are a sapphic couple.
- Kipo and the Age of Wonderbeasts: Key character Benson is gay and starts a romance with Troy.
- The Legend of Vox Machina: Half of the main protagonists are bisexual or Ambiguously Bi (according to the voice-actors who created these characters, originally mainly as self-insert fantasies in a private D&D game): Scanlan will hit on anything that moves, while having a romantic crush on Pike. Vax'ildan will flirts with men and reacts favorably to being hit on by another man, while at the same time being in love with Keyleth. Keyleth returns Vax's romantic feelings (Word of Gay states she is demisexual which may explain her hesitance to start a relationship with him), but she also has a secret crush on Vex'ahlia. Vex'ahlia is slowly falling in love with Percy, but she also has some Belligerent Sexual Tension with the supporting character Zahra. There also are several major supporting characters who are explicitly queer: Gilmore (Camp Gay), Allura (femme bisexual), and Kima (Butch Lesbian) - the latter two are a couple. Season 1 villain Sylas Briarwood may be bisexual, based on his interactions with Vax, as well as his devotion to his wife. There are even a few very minor characters who are supposed to be non-binary or genderfuid, such as the shapeshifting barkeeper in Emon and the blue-haired elven rebel in Whitestone.
- Little Demon: Chrissy's human mother Laura is bisexual; she was in a relationship with Satan (which ended up producing their Anti-Christ daughter), was flirting with (and trying to kill) a male Serial Killer in "Everybody's Dying for the Weekend" and eventually had a fling with a female sea-monster in "Wet Bodies".
- The Loud House: Lincoln's best friend Clyde has two dads. And one of Lincoln's sisters, Luna, has a girlfriend named Sam. Dana (previously Donnie) Dufrense, a judge for Lola's Miss Prim and Perfect Pageant, is a trans female and transitioned after her actor also came out as trans.
- Magical Girl Friendship Squad: One of the protagonists is a Butch Lesbian.
- Middlemost Post: "To Narwal With Love" sees Russel, a female walrus, fall in love with Winfred, a female narwal.
- Miraculous Ladybug: Zoé Lee, one of Marinette's friends, is sapphic and admitted to have romantic feelings for her. Likewise, Marinette's teacher Miss Bustier is in a relationship with a woman named Gisele.
- Monster High (2022): Animated series based on the Monster High toys; the character Frankie Stein had an Adaptational Gender Identity change from cisgender girl to a femme non-binary teen, played by non-binary actor iris menas. Later in the series, Frankie starts dating Cleo De Nile.
- Moon Girl and Devil Dinosaur (2023): Casey, one of the main leads, has two dads, Antonio and Isaac, and Brooklyn, a volleyball player at her school, is a transgender girl.
- My Little Pony: Friendship Is Magic: Two of the Ponyville citizens, Lyra Heartstrings and Sweetie Drops, are a lesbian couple who get married later on. Scootaloo also has two aunts, Aunt Holiday and Auntie Lofty, who are a lesbian couple.
- Nate Is Late: Malika, one of the two main characters, has two moms.
- OK K.O.! Let's Be Heroes has a sheer amount of queer characters. One of the main characters, Enid, is bisexual and falls in love with Red Action, a lesbian. The primary villain, Lord Boxman, is pansexual and gets together with another major villain, Professor Venomous, who is bisexual. Supporting characters Nick Army and Joff the Monk are clearly gay, and are even shown getting married in the series finale. Minor character Gregg is non-binary.
- Onyx Equinox: The main character, Izel, was confirmed to be gay and does have Homoerotic Subtext with one of his party members Yun. Meanwhile Yaotl is bisexual, the festivities as his sacrifice to Tezcatlipoca abundantly showing him having intercourse with other men. Originally Zyanya was designed as a Muxe, a Zapotec third gender, but this unfortunately did not come to pass.
- Oswaldo: Oswaldo's friend Leia has two moms, one of whom is also the principal of their school.
- The Owl House: The main character, Luz Noceda, is bisexual. While romance isn't the main focus of the show, several episodes are driven by her growing relationship with the young lesbian witch Amity Blight, which eventually becomes canon. In addition, major character Eda Clawthorne has a non-binary love interest called Raine Whispers. Supporting character Willow has two fathers, and Eda's older sister Lilith Clawthorne is aroace (aromantic and asexual).
- Peepoodo & the Super Fuck Friends: Peepoodo falls in love with male musician Tonio in one episode. Kevin the polar bear struggles with his identity in another, soon after finding herself and coming out as a trans woman named Evelyn.
- The Proud Family: Louder and Prouder: 2020s Revival of the original 2000s The Proud Family series; one of Penny's friends has two fathers (and they're the first characters in Disney Television Animation to kiss onscreen), and her other friend Michael is gay (which was only alluded to in the original series) and gender non-conforming.
- Ren & Stimpy "Adult Party Cartoon": Ren and Stimpy are depicted as a couple in this series.
- Rick and Morty: Most of the main characters are explicitly pan/bisexual or have at the least shown attraction to both men and women. In particular: Rick Sanchez identifies as pansexual and used to have feelings for his best friend Bird Person, and in the past had a relationship with a Hive Mind that took control of bodies of all genders; Jerry Smith had a threesome with his wife and another man and even had a fantasized male partner in one episode; and Beth Smith spends an episode having an affair with a clone of herself, who is still female.
- Ridley Jones: Fred is non-binary and Ismat has two dads, Aten and Kosy.
- Rugrats (2021): Betty is a lesbian in this version.
- She-Ra and the Princesses of Power: Almost every character has been shown to be queer to a degree; For example, Glimmer and Bow are both bi and the latter is raised by gay dads, and arc antagonist Double Trouble is an androgynous non-binary shapeshifter. The core of the show is Adora's Foe Romantic Subtext with Catra which eventually became canonical romantic subtext when Catra had a Heel–Face Turn in Season 5, eventually ending with the two declaring their love for each other and kissing in the show's final episode.
- The Simpsons: Smithers, Mr. Burns' right-hand man, is openly gay in newer seasons and closeted in earlier seasons. Patty, one of Marge's older sisters, is later revealed to be a lesbian. A few other supporting characters like Mr. Largo and Julio are also gay.
- Solar Opposites: The Shlorpians are genderless Plant Aliens who nonetheless are able to pick the gender they want to be identified as. The two main characters Korvo and Terry are a bisexual Shlorpian couple who both identify themselves as male and are in an open relationship, which is taken more seriously in later seasons.
- South Park: Mr. Garrison comes out as gay in the early seasons after being an Armoured Closet Gay, spends a few seasons identifying as a transgender woman (though the show doesn't depict this flatteringly) before identifying as a gay man again. Other recurring gay characters, who frequently show up to satirize issues of sexuality, are Big Gay Al and Mr. Slave. In later seasons, Craig and Tweek are gay and dating each other, with their reputation as a gay couple being a talk of the school despite them being casual about it.
- Star Trek: Lower Decks: Sexuality is pretty fluid on the U.S.S. Cerritos. Ensign Mariner is openly pansexual, and many of the Mauve Shirts, such as Barnes and Stevens, are Ambiguously Gay or Bi to varying degrees.
- Steven Universe: The Gems are a non-binary alien race who all present feminine and use she/her pronouns, meaning that any attraction between them is homosexual by default. In particular, Ruby and Sapphire, Garnet's component gems, are permanently fused out of love for each other. They even broke ground by having an on-screen wedding and kissing. Steven can fuse with his girlfriend Connie to form a non-binary, intersex fusion, Stevonnie. Most of Steven's other fusions are non-binary and use they/them pronouns.
- Steven Universe: Future: Sadie's love interest, Shep, is a non-binary human who exclusively goes by they/them pronouns.
- Strawberry Shortcake: Berry in the Big City: Lime Chiffon has two dads, Strawberry Shortcake’s aunt, Aunt Praline is sapphic and in a relationship with Kiki Keylime. Mr. Mangosteen, a fruit stand vendor, is gay and engaged to Bobby Bananabee. Banoffee, a fashion model, is a transgender woman. Raisin Cane, a band member of the “Sherry Bobbleberry and the Berry Fairies” had an Adaptational Gender Identity change from cisgender girl to a non-binary kid, played by non-binary actor Justin Saint.
- ThunderCats Roar: Panthro's love interest, the Bat Queen, is bisexual, and exes with the Rat Queen.
- Total Drama: The 2023 reboot introduces the first openly gay contestant, Bowie. He ends up in a relationship with Raj, which functions as Raj's Coming-Out Story.
- Transformers: EarthSpark: The Terran transformer Nightshade is a Non-Human Non-Binary (voiced by non-binary actor Z Infante) that uses they/them pronouns, in contrast to other transformers that assign themselves male or female. Their gender identity is affirmed by a non-binary human character named Sam (she/they).
- Tuca & Bertie: The lead characters are both bisexual.
- Twelve Forever: The lead, Reggie, is revealed to have a crush on a girl at her school. Also two of the residents of Endless are a gay male couple.
- Velma: Velma, the protagonist, and Daphne are bisexual, while the latter was adopted by two lesbian cops.
- Vida the Vet: The title character has two fathers.
- Voltron: Legendary Defender: One of the main characters, Shiro, is revealed to be gay towards the end of the series; he had previously been shown to have had a past male partner, their relationship is ambiguous within the show itself with Word of God specifying him as gay. The series finale rectifies this as the episode briefly shows him getting married to another man, complete with a kiss.
- Young Justice (2010): Animated series about a group of teenage/young adult superhero sidekicks that run covert missions for the Justice League; the series has several LGBTQIA+ characters both main and supporting, specifically Aquaman/Aqualad who is queer and has romances with female and male characters. Lagoon Boy is also queer and is in a polyamourous relationship with a woman and a man. Halo (an alien Motherbox in the body of a human girl) is a Non-Human Non-Binary of sorts and starts to use they/them pronouns in the fourth season. Kid Flash and El Dorado are Word of Gay examples, as their sexuality/relationship was never outright stated in the series.
