- After the tragic 2016 Pulse nightclub shooting, which claimed the lives of several Universal Team Members (Jonathan Camuy, Anthony Laureano, Xavier Serrano, Shane Tomlinson, Luis Vielma, Jerald Wright), Universal Orlando held a employees-only memorial event the following Thursday that paid to tribute all those who had fallen; and broadcast the event to the employees at the Universal parks in Hollywood, Japan, and Singapore. Along with that, they also began to use the slogan, "Love is Universal", around the company. Later on in September of that year, Universal further paid tribute to the victims by placing their names on a window (pictured) that can be seen near Universal's Horror Make-Up Show.
- Any little kid that's overwhelmed with joy when they first meet the likes of Spider-Man, SpongeBob SquarePants, The Simpsons, Optimus Prime, or a Minion. Or going through "The Wizarding World of Harry Potter" or the Despicable Me: Minion Mayhem ride.
- The ending of E.T. Adventure, with the titular character wishing you farewell (and actually saying your name!)
- If you're walking through the exit hallways at the right time you'll hear "I'll be right here..."
- There's also the ending of Despicable Me: Minion Mayhem, where Gru saves the girls, reveals to the trio his 1st Anniversary gift to them and happily declares how much he loves them.
- The ending to Back to the Future: The Ride, while quick, somewhat qualifies too, just because of Doc's final words to the riders:Doc Brown (gleefully): You did it! Not only did you catch Biff and save the universe, but you proved that my latest invention is a success! Go forth time travelers, and remember the future is what you make it!
- The Universal 100th show, which is essentially a giant and lovingly-crafted homage to the company's legacy of films. It's especially moving to any big film fan or historian.
- The fact that the Studio Tour queue and its starting area contains tribute posters to some of their oldest films, like The Phantom of the Opera (1925) and All Quiet on the Western Front, plus featuring the latter in a homage video to all their Best Picture winners, shows that Universal still loves them very much, and hasn't forgotten about them in favor of more popular films like Despicable Me and Jurassic Park.
- At the end of Transformers: The Ride, Optimus Prime thanks the riders for their bravery during the mission. For any Transformers fan, it's just beautiful to see their childhood hero thank them.
Optimus Prime: Evac believes you can help him protect the AllSpark. I believe you can, too. So the fate of the earth is in your hands.
- Heck, just Prime having unconditional faith in the riders:
- The Simpsons Ride's Springfield area has Moe's Tavern, with a 2nd level that shows drawings of the many guest characters from the show, but the heartwarming part comes with two pictures: Homer embracing his late mother, Mona, and Krusty with his daughter.
- As on the television series, A Day in the Park with Barney ends with "I Love You," an already heartwarming song to fans of the purple dinosaur. As an added touch, hearts come down from the ceiling during the song's second stanza. In other words, the show ends with love literally in the air.
- Following the death of Stan Lee in November 2018, fans created a makeshift memorial in the entrance of the Spider-Man ride at Islands of Adventure.
Heartwarming / Universal Studios