When Ram finds out that Flynn is a user just before he derezzes, finally knowing that the users have not abandoned their followers and that there is hope for the system after all.
When Tron discovers Yori, a shell of her former self and unable to recognize him. He grabs her, transfers some power into her, and she snaps out of it, hugging him with relief.
The cut scene where Yori brings Tron back to her home. She shows him that she still has a playful and rebellious streak (like her User counterpart, who essentially splits the difference between the overly-serious Alan and the less-than-serious Flynn), turning the barren apartment into a beautiful paradise for him and then taking the time to... interface.
The I/O Tower. You hear of Programs speaking of the Users like we speak of Gods, but when Tron finally steps into the I/O beam and connects with Alan, the sheer joy on Tron's face truly delivers how important and powerful the need for Programs to contact their users is.
Dumont:(happy, amazed and proud) The city is lighting up!
Gibbs is the stand-in for every single computer pioneer who ever lived (a late middle-aged man in 1982) who still has the streak of rebellion and vision. His actor Barnard Hughes went from complete confusion over the arcade game craze to a hero for young programmers, something he was proud of up until his death in 2006.
When Flynn explains to Tron and Yori that Users are just as fallible as Programs. While disbelieving at first, Tron seems to take the revelation in stride, perhaps because he now knows that both groups have something in common.
Tron: If you are a User, then everything you've done has been according to a plan.
Flynn: You wish. You guys know what it's like... you just keep doin' what it looks like you're supposed to be doin', no matter how crazy it seems.
Tron: That's the way it is for Programs, yes.
Flynn: I hate to disappoint ya, pal, but most of the time, that's the way it is for Users, too.
Tron: (more amazed than disappointed) Stranger and stranger.
The ending scene. Alan and Lora are waiting on the rooftop helicopter pad for "the boss." Copter lands, and Flynn rushes out, gives his pals a hug, and off they go. The last shot is the Los Angeles skyline fading from daylight to nighttime. The dark sky, bright lights, and neon make the human world look almost indistinguishable from cyberspace, silently saying that Users and Programs are not so different.
Ram warmly expressing what he used to be "before": a Program who served an insurance company. It's a crowning moment of funny as well, but it's also touching to know a computer program is just as enthusiastic about genuinely helping people as the people who created him.
It's even better with the Expanded Universe as Roy Kleinburg turns out to be just as much of a idealistic, friendly, and caring guy as the software he coded.
Barnard Hughes noted in the "Making of..." documentary that he became a Cool Old Guy to pre-teens and teens thanks to TRON, which pleased him to no end. It made him investigate the new technology closer.
Dan Shor stated in the documentary that whenever he tells someone that he's an actor, he always gets asked, "Oh? And what have you done?" When he tells them, "I did TRON", the reaction always turns to amazement and jealousy.