- When Harry, in his hospital bed, is told he has good friends, and he answers, "My best friends." He says it with all the earnestness of a contented 8-year-old, and it's just so cute.
- Harry's arrival just in time to help Peter take on the Sandman and Venom. Peter's no longer fighting alone and is now part of a superhero duo. And then afterwards, his and Peter's conversation just before his death, which also counts as a Tear Jerker.
- Peter: I should never have hurt you... said those things...
Harry: (gentle and earnest) None of that matters, Pete. You're my friend.
Peter: (smiling tearfully) Best friend.
- Spider-Man 3 may be a fairly polarizing film, but damn if everyone didn't well up a bit at the "Birth Of Sandman" scene, watching Sandman at one moment barely able to stand up to forming a full body with steely determination after just one look at his daughter. The beautiful musical score and jaw-dropping visuals (When you can get a pile of sand to be on both the verge of tears and have a look of absolute determination, you're doing something right) only adds to it.
- After Peter has shed himself of the alien symbiote and sits alone depressed in his apartment, Aunt May visits Peter to see how things have gone between her nephew and his fiance and asks if he's proposed to her yet. Peter tells his aunt that he's not ready for marriage because he had hurt Mary Jane badly and returns the proposal ring Aunt May gave him earlier in the movie, much to Aunt May's shock. In response, Aunt May replies by saying that Peter should forgive himself and that Aunt May believes Peter will do the right thing because she knows he's a good person and she knows he will find a way to make things right again and to give it time. This scene also serves as a Tear Jerker moment.Aunt May: Well, you start by doing the hardest thing: You forgive yourself. I believe in you, Peter. You're a good person. And I know you'll find a way to put it right... in time.
- The ending, where Peter and Mary Jane get back together again. Ever since his childhood, Peter wanted to be with Mary Jane, and being Spider-Man meant that it was all but impossible. Yet, Peter has finally managed to balance his personal life and his life as a superhero, conquered his dark side, made peace with his past by forgiving Sandman, and finally, after all he's gone through, he can spend his life with the woman he loves. The series began with him wanting to be with Mary Jane, and it ends with the two of them together.
- Even more than that: they've both just lost their mutual best friend and put each other through hell, but he comes back to her, and there's the implicit recognition when she takes his hand to dance with her that he won't let her suffer alone, and they'll work through their issues together. Bottom line: when all is said and done, they love each other.
- The scene is done without much fanfare, notwithstanding MJ's singing (which stops when she notices Peter). The two lock eyes, reach for each other's hands, and hug. No declaration of lovenote , no apologynote , no Big Damn Kissnote . There is something beautiful in its simplicity.
- It's even better meta-wise: in the planned fourth film, Peter and MJ would have been married for several years, before separating at the end, as per the demands of studio executives. With the cancellation of Spider-Man 4, Raimi (who hated the planned draft for the fourth film) just saved the couple a lot of trouble, closing out the trilogy with their reconciliation.
- The novelization features the original ending for the film. Harry and Peter are trying to defeat Sandman and Venom and even with the added help are having trouble. Then Sandman's daughter and ex-wife show up and beg Flint to stop, with his daughter telling him that he isn't a bad person. Flint returns to his human form but Venom begins to go on a rampage. Peter tells Flint to run and get his family out of there and the three leave.
- Mr. Ditkovich, Peter's apartment landlord, brushing off the angry outburst Peter (who was under the symbiote's influence) just dealt on him, assuring his daughter that Peter is a good boy, and then quickly forgiving Peter later on when the latter comes to apologize. Furthermore, Mr. Ditkovich lends Peter some romantic advice upon learning that Peter plans to propose to Mary Jane. Heartwarming in retrospect, when one considers his portrayal from the previous films as a bullying asshole.
- While it's a bit sad considering how Peter is stringing her along and she's lapping up the attention she's getting from him, it's sweet that Ursula is catering to Peter's every whim and stuffing him with an endless buffet of her homemade cookies.
- Also, despite her own crush on Peter, Ursula still supports Peter and Mary Jane together full-stop, urging him to call her earlier in the movie, and later being giddy when Mary Jane calls him because she thinks it means that the two of them are about to get engaged to each other. In the novelization, it's Ursula who actually helps get Mary Jane the singing waitress job after her acting career falls through.
- Gwen Stacy, who is shown throughout the film to be nothing more than a beautiful person on the outside and inside. She is smarter, richer, and generally portrayed as a better partner for Peter than MJ, but she respects their relationship and makes a point to ask whether Peter wants to avoid MJ after their messy breakup during their date (he intentionally refuses). And when she realizes the real reason why Peter asked her out, she apologizes to MJ in person and leaves Peter in disgust.
- Bruce Campbell's cameo is both funny and heartwarming, in contrast to his other characters who either mock or impede Peter he is instead thrilled at the idea of Peter proposing to MJ at his restaurant and deeply honored to be asked for his help in the matter.
- In perhaps the best of Stan Lee's cameos (and Stan the Man's personal favourite), he muses on the impact of Spider-Man in fictional New York to Peter Parker. It's easy to interpret it as Stan talking about the greatest lesson comic book superheroes (particularly Spider-Man) try to teach their readers:Stan Lee: You know, I guess one person can make a difference. 'Nuff said.
- Peter finally forgiving Flint after learning that his shooting of Uncle Ben was an accident (caused by Carradine, so Peter still shares blame for not stopping him when he had the chance), and that Flint actually didn't flee with Carradine in Uncle Ben's car because he stayed behind at Uncle Ben's side and he was the one who called for help, explaining the crowd of people around the dying Uncle Ben when Peter arrived on the scene.
- The scene added to the Editor's Cut where after reforming, Sandman forms into a sand castle near Penny is a touching scene that helps to humanize Flint.
- Aunt May's reaction to Peter telling her that he plans to marry MJ counts.Aunt May: Peter, what is it?
Peter: It's MJ. ...I'm gonna ask her to marry me.
Aunt May: (gasps in joy as she hugs him) Peter...!
Aunt May: It was a beautiful day and he said, 'Let's swim to the island.' And at the island, we found a perfect spot by an old tree. And we laid down, and looked up at the sky. And then he said, 'Close your eyes, May,' and I did, and then he said 'Open them!' and I did. And he was holding this ring (shows her ring) dazzling in front of me. I thought it was the sun.
- This leads to a whole scene about the subject, in which May tells Peter the very sweet story of Ben's proposal to her, then gives Peter the same ring Ben used.
- In the game, there is a part where Jameson almost thanks Spiderman for saving him. Even though he stops himself from saying it half-way through, Spiderman still tells him "You're welcome".
Heartwarming / Spider-Man 3