- The very wholesome interactions with the Mane 6 (and Spike) with the Autobots.
Spike: He called me... me... inspiring...
- As Bumblebee and Optimus fall from the sky, Optimus states he'll cushion Bumblebee's fall. Twilight replies that it won't be necessary as she uses her telekinesis to help their descent.
- Rarity and Arcee bonding as they ask each other questions of each other's worlds.
- Spike gushing over Grimlock for being amazing and Grimlock returning the favor.
- Pinkie Pie and Gauge cooking foods for each other to share... even if they aren't compatible with each other.
- Fluttershy befriends the Mini-Cassettes by giving healing ointment on their wounds. She was able to get Soundwave to make peace — well, Discord threatening to turn him into a pony also helped — to the point that when Megatron messaged Soundwave rendezvous, he decides to stay a little bit longer.
Heartwarming / My Little Pony/Transformers: Friendship in Disguise!