- David Letterman was a guest on a 1994 episode. Conan credits Letterman agreeing to appear on his show as the moment when Late Night began to turn it around, and is still grateful to this day.
- Conan bringing out a duck was a genuine "awwww" moment. He didn't even have a sketch for the duck (whom he dubbed "Quackers"); he just thought America could use cheering up and it personally made him happy to see it wandering around the set. Of course, we all know what happened next... Note
- Conan's first show after 9/11 in which he revealed that he was so shaken by the tragedy that he did something he hadn't done in 8 years—went to church and prayed. As he sat in St. Patrick's cathedral (just a block or two from Rockefeller Center, where Late Night was taped). He realized that this beautiful building was still standing and that despite the horrible event, there was still a lot of beauty in the world. This was the first time he urged his viewers not to be cynical.
- In the episode where the alarm noise kept interrupting the show, Conan apologized to his guest Paget Brewster for the inconvenience. Her response is both funny and makes you go "awwww":Paget: I got to look at you in person, Conan, I'm fine.
Conan: You're my favorite guest ever.
- His interview with Gene Wilder is very touching. The two discuss Gene's (at the time) new book and towards the end of the interview we get this heartfelt exchange between the two.Conan: You've been through so much. You've had incredible career success, you've had high highs, you've had tragedy in your life, and when you get to the end of your book, you seem like you're in a really good place now and you're happy. And that made me feel good because I want you to be happy. And that's true, right? You feel good.
Gene: (taking Conan's hand) I'm happier than I've ever been in my life.
Conan: That's fantastic.
Heartwarming / Late Night with Conan O'Brien