- The squirrel giving Dukey a nut with a birthday candle in it in "Bath Time For Johnny" because Johnny "forgot" Dukey's birthday and Dukey was in tears.
Dukey: You didn't bathe for five weeks and rubbed fish on yourself for me?
- From the same episode:
Johnny: There isn't anything I wouldn't do for you!
- When Johnny and the johnny-Stopping-Force-Five are about to perform a Heroic Sacrifice near the end of 'JX 5 The Final Ending', Johnny wants Dukey to take Jillian in the escape pod off of Dark Vegan's ship. Dukey would rather go out with Johnny;Dukey: Thanks for picking me up from the pound that day.Johnny: And thank you for being the best dog a boy could ask for!
- From the episode "Johnny Test in Black & White":Susan and Mary: And we love you! (Hugs Johnny) Don't get used to this.
- Gil's positive attitude towards Johnny, always referring to him as "Broham"
Heartwarming / Johnny Test