Aaaand...there was the first Nerd vs Critic battle, when they fought with light sabers, but the Critic accidentally had his upside-down...
And during his series, video game confessions, where he's a bartender at a restaurant that sees many video game characters, Samus is apparently the giver of 2 crotch shots, and I mean "with the lazer gun" shot, to both Justin Bailey, and Leisure Suit Larry, because she hates it when people ogle her.
In Kickassia, the Critic finally got to do this to someone else, showing Lee one of the few downsides of being three-dimensional.
In Nostalgia Critic's review of Casper, an animated Casper infuriated by the Critic's panning of his film states that if he can't have a lasting impact on popular culture, he can still have one on his balls. Cue Groin Attack.
In the "You're A Dirty Rotten Bastard" Christmas Special, before meeting his guardian angel, the Critic clamps the poor guy's balls.
Happened a few times in Suburban Knights, especially with Malachite using magic to force Nostalgia Critic to hit himself in the balls, 15 times.
After viewing Zardoz, Oancitizen takes the "Gun is Good, Penis is Evil" message too literally and goes to the bathroom to shoot off his groin. He somehow misses.
At the end of the Nostalgia Critic's review of Man of Steel, Superman opts to defeat Zod by Heat Vision castration instead of killing him.
In the NC's review of Nicolas Cage's The Wicker Man (2006), in order to get Critic to reference the infamous NOT THE BEES scene, Tamara holds onto him while Malcolm hits his groin with a baseball bat. Every time the Critic is hit, his voice gets higher.
The Angry Joe Show: At the end of his review of the Deadpool game, Joe is confronted by Deadpool, angry at dissing his game. Joe manages to convince him the game was lacking... in kicking guys in the balls. Deadpool agrees, and thanks Joe... with a punch to the nuts. After that, Joe swears to not review any Deadpool game again.
A series of videos on YouTube entitled "Kicked in the Nuts" involves a man in an orange afro wig kicking (supposedly) unsuspecting people in the nuts. They invariably react at first with pain and/or anger, until he points at the camera and informs them that "You've been kicked in the nuts!", at which point they're just happy to be on camera.
Dr. Horrible's Sing-Along Blog: As part of a montage of Captain Hammer's jerkass humiliations against a helpless Dr. Horrible, he gives the doctor both a knee to the groin and an extremely painful-looking wedgie.
Game Grumps: While the action itself wasn't shown on camera, Danny recounted one time where he met Matt Thompson (One of the executive producers for Archer) at a convention, where Matt was so drunk he punched Danny right in the dick. Though he later sent him an apology e-mail after he sobered up, and they met in much better circumstances a few times after that.
Played for Drama:
The second installation of Awkward. contains two in quick succession. First Alex against Lester in a staged fight to make Kevin look like a hero (which later leads to some unforgettable mental imagery when Lester remarks, "It's like a cat tried to suck my dick."), then Henry against Kevin. He takes a run at it.
In the KateModern episode "Back on Brick Lane", Gavin, who is having a mental breakdown, chases after and pounces on Jennifer - a complete stranger - mistaking her for Kate. Jennifer knees him in the groin and runs off, yelling hysterically. Gavin lies on the ground, coughing and sobbing.
Bruiser defeats Peaches in the first video by kicking her square between the legs.
Peaches suffers it again in the second video, this time from Shizuka except the second time, she's faking the pain — she learned from the first encounter and trained specifically to counter that particular tactic.
In the third video, one of the Slugger Squad members gets in a cheap shot on Knuckles by kicking her in the crotch, from behind, defeating her in the process.