From his team-up with Daredevil. "Thank God for Stilt-Man!"
Issue 16 of Avenging Spider-Man sees him unwillingly team up with the X-Men, where he deals with a human/spider hybrid pretty brutally (but non-fatally). When Wolverine challenges him, Otto simply puts him down HARD - leading to his trying to talk his way out of getting mind-scanned by Rachel Grey to explain his out-of-character behaviour. We get multiple gems here:
Otto identifying Wolverine's super-senses as "badger senses, or whatever you've got."
His pointing out that at least when he acts out of character, he doesn't set the world on fire, unlike every Phoenix comic ever.
His final sally, where he explains beating up Wolverine as... well, can they honestly say they haven't thought about doing it at some point?
Issue 4 was funny from start to finish, from Otto learning with displeasure that Peter is not a doctor to Ghost Peter snarking about Otto's age.
Also in Issue 4 when Modell and Jackson check up on Otto's mad science experiment, his Robot Buddy — a reprogrammed Living Brain — asks if they want tasty beverages which they decline, a few pages later:
At one point during a team-up in A+X #14 Otto responds badly to Magneto's pop-psychoanalysis by screaming that perhaps Magneto hasn't experienced tragedy on the scale that Spider-Man has... revealing himself as completely unaware that Magneto is a Holocaust survivor. When Magneto simply deadpans his reply, a wide-eyed Otto only stares before hanging his head in shame.
After Peter regains control of his own body, the Green Goblin notes that he is going to leave him (thinking he's talking to Otto) with nothing. The way the real Spider-Man reintroduces himself is hysterical.
Peter running into Miguel O'Hara after regaining control of his body - Peter's confused at Miguel's presence in 2014, whilst Miguel starts tearing into Peter over leaving the battle so he could change his costume. Peter promptly sums up the whole "Freaky Friday" Flip and Miguel's response is to note that the situation sounds "just stupid enough to be right."
In #9 of the Team-Up series, Otto misremembers what Anna Maria told him about "the bullet that killed someone named "Two Packs" that apparently kicked off some bi-coastal wrapping war."
While he was chastising the then-new second Nova (Sam), Otto attempts to channel Peter's usual quips and pop culture references. He ends up sounding like... well an old man struggling to grasp "youth culture", with a failed attempt to reference Justin Beiber and then a brief rant on teen pop stars. Sam's reaction to SpOck's behavior in general.
Anna Marias realization that Otto Octavius is the only hero around to save San Francisco from a rampaging cosmic terror in #2, after being reminded that the avengers are off-world at the moment:
Anna Maria: Okay, call Peter Parker. Even if he cant get here in time, he knows who can. Captain Marvel. The X-Men. Hell, even Deadpool
living Brain: I am unable to reach Mr. Parker or any of the enhanced first responders.
Anna Maria: Oh God. Please dont tell me that the only thing standing between San Francisco and agonizing death is Otto freakin Octavius?
Everything about Otto as Eliot Tollivers date in #5
Since Anna Maria told him to be honest in his relationships, and Ock has no idea how to people, he tells her that he is secretly Otto Octavius in the middle of their first date. It goes about as well as youd think.
After his date gets possessed by a demon forcing him to fight and restrain her.
Superior Spider-Man: I am beginning to reconsider dating.
San Francisco is being invaded by frost giants. Seeing as Superior Spider-Man cannot make contact with any legitimate heroes, he has chosen the West Coast Avengers to aid him (his words). So he proposes a plan to take the fight to the giants' realm, and defeat them there.
Gwenpool: Whoa. Hold on. That's not how this works. It's a crossover event. And we're an ancillary tie-in. We do our little part of the story, score a small victory and then go back to our regular narratives.
Superior Spider-Man: This woman is quite mad.
Fuse: Ignore her. She thinks this world is fictional and she's the only "real" person here.