Anne using the Internet to research BDSM and getting freaked out by what she finds.
Although the end of chapter 4 is very serious Ally recounting how the doctor that treated her fractured wrist got a lot of mileage out of her Naughty Nurse Outfit is a much needed bit of levity.
When Lisa starts up a new erotica series to get over the break-up with Ally, she eagerly awaits the comments that would follow her new story. Instead she gets post after post of clueless fans wondering when she'll write more Lisbeth stories. The look on Lisa's face: priceless.
Well, that and the usual trolls. But the "Where's Lisbeth?" posts overwhelm the trolling by 10-to-1.
The completely atrocious singer that Harper hires for the party at the Crimson on New Years Eve.
Ally having two left feet:
Ally: I'm a horrible dancer!
Lisa: That's okay! It's a horrible song!
At the end of the fifth arc, showing future scenes from Lisa and Ally's relationship, we see a scene of Ally looking like a sulky teenager while Lisa orders her (for as yet unknown reasons) to talk to her mother.
Lisa: You will talk to your mother like a grown up or I will! And trust me, you don't want that!
In the prequel series Mercy, flashbacks to Ally and Alan's start of their dom/sub relationship shows that they flipped a coin to see who would be the dom(me). It goes to Alan... who suddenly gets overwhelmed by the situation and panics.
They also bicker over what the Safe Word will be, and Alan goes with "Mercy" for his. When Ally argues how cliched that one is, Alan turns on his Inner Dom and says "You will say Mercy or I will show you none," it so excites Ally that she turns on her Inner Domme and ordershim to keep going.
It also works as a bit of Foreshadowing how their relationship ends and how Lisa ends up with Ally: Ally is a Dominatrix at heart.
The April Fools one-shots. Especially the 2016 one, where Sejic goes full troll and draws Furstone, a Furry cat-themed version of the comic.
The 2019 April Fools, a direct sequel's from 2018, has Hamster reviving Sejic via the use of a Pet Cemetery in order to draw furry fetish comics to Hamster's specific commands. Unfortunately, Sejic suffers flashbacks to his death, ruining Hamster's dream of millions and forcing him to reclaim Sejic's thumbs. And then it gets seriously weird...