Ace Attorney: The four main protagonists of the series can be considered this, particularly in the final episode of Spirit of Justice, where they all team up. Phoenix Wright is phlegmatic (relatively laid-back and easygoing, often takes abuse from annoying witnesses without retaliating, but is also very selfless and kindhearted); Miles Edgeworth is melancholic (reserved, rational, values finding the truth above all else); Apollo Justice is choleric (Hot-Blooded, passionate, never gives up); Athena Cykes is sanguine (enthusiastic and optimistic).
Sanguine: the cheerful, light-hearted, impulsive Imoen.
Choleric: the hot-headed, highly motivated, rage-harnessing Large Ham Minsc.
Melancholic: the cautious, sarcastic, grieving widow Jaheira.
Phlegmatic: the easy-going yet reserved Yoshimo.
In Blaze Union, the first five party members: Garlot (choleric), Jenon (melancholic), Medoute (phlegmatic), Siskier (sanguine), and Aegina (eclectic). And after the party dynamics suffer an upheaval, things shake down like so: Gulcasa and Leon (choleric), Nessiah (melancholic), Baldus (phlegmatic), Emilia (sanguine), and Aegina is... still eclectic.
Choleric: Gehrman, The First Hunter. Subsequently The Mentor of the Hunter, but is also a broken-hearted pervert, who guides you to uncover the mystery of the story. Before offering you a release from the dream.
Phlegmatic: The Doll. Beautiful, elegant, caring yet mysterious. She aids The Hunter in levelling up, developing feelings for them overtime. She is Gehrman's creation, who serves him in more ways than one.
Camp Buddy: Keitaro's potential love interests; Hiro (Sanguine), Yoichi (Choleric), Hunter (Melancholic), and Natsumi (Phlegmatic).
Catherine, in terms of Vincent and his three bros that he hangs out with at the Stray Sheep. Orlando (choleric), Jonny (melancholic), Vincent (phlegmatic), and Toby (sanguine).
The Colour Tuesday: Alex is the hotheaded driver of the story (choleric). Kyle is the determined and selfless (melancholic). Bella is a slightly airy Cloudcuckoolander (sanguine). Chadd, the last permanent cast member, is a born follower (phlegmatic).
The Love Interests in Dragon Age II: Isabela (sanguine), Merrill (choleric), Anders (melancholic), Fenris (phlegmatic with a dash of melancholic).
The party in Dragon Quest VIII: Hero (phlegmatic), Yangus (sanguine), Angelo (melancholic), and Jessica (choleric).
The four playable characters of Drakengard, though at times they muddle about with their humors, tend to fall into distinct ones: Arioch (sanguine), Caim (choleric), Leonard (melancholic), and Seere (phlegmatic).
In Eroge! Sex And Games Make Sexy Games, we have Nene (Sanguine), Iori (Plegmatic), Momoka (Melancholic), and Kisara (Choleric).
In ClockUp's Euphoria, we have Kanae (Melancholic), Rinne (Plegmatic), Rika (Sanguine), and Nemu (Choleric).
Fallout: New Vegas: NCR (Choleric), Caesar's Legion (Sanguine), Mr. House (Melancholic), Yes Man (Phlegmatic).
Fallout 4: Minutemen (Sanguine), Brotherhood of Steel (Choleric), Institute (Melancholic), Railroad (Phlegmatic)
Applies to the faction leaders too. Maxson is the driven hard-charging choleric, Preston is the sociable sanguine, talking to everyone, Desdemona is the phlegmatic who acts as the Railroad's Team Mom, while Father is the cold logical melancholic.
Choleric: Lilac (the brave leader, loyal to her friends, and stubborn to a fault at times)
Sanguine: Carol (the cheerful joker, always full of energy, naive about a lot of things)
Phlegmatic: Milla (the shy but friendly supporter, always willing to lend a hand, but not as brave as her friends)
Melancholic: Torque (in the first game - the genius of the group, ready with what's necessary for the challenges ahead) and Neera (in the second - the reserved soldier, normally tolerates nothing less than order)
The unprincipled accomplice, plus Big Bad quartet (counting a Big Bad from USN scenario): repentant accomplice Reiji Sakata (phlegmatic), Driscoll (sanguine), mole genius Olson (choleric), Patrick Wingar (melancholic), and Koichi Sakata (eclectic).
Gears of War's Delta Squad are like that: Marcus Fenix (choleric), Damon Baird (melancholic), Dominic Santiago (phlegmatic), and Augustus Cole (sanguine).
Grand Theft Auto IV: The four main characters fit: Brucie (choleric), Niko (melancholic), Little Jacob (phlegmatic), and Roman (sanguine).
Grand Theft Auto V: The Main Characters Epitomise all the Temperaments each. Trevor (choleric), Michael (melancholic), Franklin (phlegmatic), Lamar (sanguine) and Lester (eclectic).
The Legend of Zelda: Breath of the Wild has the four Champions chosen to pilot the Divine Beasts. Gentle and compassionate Mipha is phlegmatic, jovial and friendly Daruk is sanguine, brash and arrogant Revali is choleric, and calm and wise Urbosa is melancholic.
Due to the author being interested with the subject, MARDEK RPG does this. The author believes everyone has a primary and a secondary temperament, though. Perhaps the clearest ingame example is Legion: a construct created by an inventor. It was animated with dark magic, with the inventor saying the incantation - due to his stuttering, though, four souls entered the body instead of one - one for each temperament.
Mass Effect and Mass Effect 3 squads are often comprised this way: Shepard and Javik (choleric), Garrus and Liara (melancholic) and Tali and EDI (phlegmatic). Likewise, James and Ashley (sanguine) and Kaiden (neutral phlegmatic).
Mass Effect 2 has the starting four. Shepard (choleric), Miranda (melancholic), Jacob (phlegmatic), and Joker (sanguine).
The four council races also fall into these types. Humans are the impatient git'er'done cholerics, Turians are the rigid, structured melancholics, Asari are the diplomatic consensus seeking phlegmatics, while the Salarians are the always inventive, always curious sanguines.
Mega Man (Classic) series: Rock/Mega Man (phlegmatic), Proto Man/Blues (melancholic), Forte/Bass (choleric), Roll (sanguine), and Duo (eclectic).
The Robot Masters from Mega Man Powered Up: Cut Man and Fire Man (choleric), Ice Man and Time Man (melancholic), Guts Man and Elec Man (phlegmatic), and Bomb Man and Oil Man (sanguine).
The Robot Masters from Mega Man 11: Block Man and Blast Man (choleric), Fuse Man and Acid Man (melancholic), Tundra Man and Torch Man (phlegmatic), and Impact Man and Bounce Man (sanguine).
Mega Man Battle Network: Dex and his Navi GutsMan.EXE (choleric), Chaud and Glyde.EXE (melancholic), Mayl and MegaMan.EXE (phlegmatic), Yai and her Navi Roll.EXE (sanguine), and Lan (eclectic).
Mega Man X Maverick Hunters of X5 onward: The fast-action Anti-Hero Zero (choleric), the conscientious defender X (melancholic), the father-figure Signas (phlegmatic), the flamboyant Axl (sanguine), and Douglas (eclectic).
Lady reploids of X8: Alia (choleric), Palette (phlegmatic), and Layer (eclectic).
The lone X-hunter (in his own right), X2's X-Hunters, and the Big Bad Sigma: Vile (super choleric), Agile (melancholic), Serges (phlegmatic), Violen (sanguine), and Sigma (eclectic).
Combo Ensemble counting the Four Guardians and the data-storing boss: The leaderly and loyal Phantom (eclectic), the indeterrable and Trigger Happy Fefnir (chol-sang), the virtuous and serious Harpuia (mel-chol), the calm-but-playful Leviathan (phleg-mel), and the Gadfly Maha Ganeshariff (sang-phleg).
Other bosses in 1st game: Copy-X (chol-mel) note a copy of X, but not quite the same temperament, and a lot less mature and Herculious Anchortus (phleg-sang).
Bosses in 2nd game: fiery Phoenix Magnion (choleric), Hyleg Ourobockle and Poler Kamrous (melancholic), Burble Hekelot (phlegmatic), electric Panter Flauclaws and Elpizo (sanguine), and Kuwagust Anchus (eclectic).
X-Series enemies holographed by Phoenix Magnion in Zero 2: Vile (choleric), Agile (melancholic), Bit (phlegmatic), and Colonel (sanguine).
MetroidPrime 3: Corruption: Samus (eclectic), Rundas (choleric), Ghor (melancholic), Gandrayda (sanguine), and most other allies (phlegmatic).
The main members of Diamond Dogs in Metal Gear Solid V: Big Boss (aka Venom Snake) & Quiet (phlegmatic), Revolver Ocelot (melancholic), Kazuhira Miller (choleric), Paz (sanguine), Huey Emmerich (eclectic).
The CO's from Yellow Comet certainly follow the temperaments, with the honorable and reckless Kanbei as the choleric, the strategist and serious Sonja as the melancholic, the eccentric yet calm and good-natured Sensei as the Phlegmatic and the boisterous and fun Grimm as the sanguine.
In No, Thank You!!!, we have Haru (Sanguine), Hiroshi (Leukine), Ryu (Choleric), Kouichi (Melancholic), and Maki (Phlegmatic).
Octopath Traveler: Each of the eight travelers are divided into two groups of four; The Noble characters and the Rogue characters:
The Noble Characters:
Sanguine: Tressa Colzoine (The bubbly merchant seeking adventure)
Choleric: Alfyn Greengrass (The passionate and extroverted apothecary)
Melancholic: Olberic Eisenburg (The warrior haunted by his past and looking for a purpose in life)
Phlegmatic: Ophelia Clement (The calm and kind-hearted cleric)
The Rogue Characters:
Sanguine: Cyrus Albright (The optimistic scholar who is passionate about sharing his knowledge)
Choleric: Primrose Azelhart (The dancer bent on revenge for the murder of her father)
Melancholic: Therion (The snarky antisocial thief)
Phlegmatic: H'aanit (The emotionally reserved hunter who respects nature)
Choleric: Ryuji Sakamoto. Proud, aggressive, and blunt.
Melancholic: Yusuke Kitagawa. Artistic, sensitive, and critical.
Phlegmatic: Ren Amamiya. Indifferent, teasing, and stubborn.
Females Party Members:
Sanguine: Ann Takamaki. Friendly, happy-go-lucky, and sympathetic.
Choleric: Makoto Niijima. Judgmental, irritable, and overbearing.
Melancholic: Futaba Sakura. Anxious, straightforward, and reclusive.
Phlegmatic: Haru Okumura. Gentle, benevolent, and reserved.
Intentionally invoked in Planescape: Torment. In the end game, you meet up with three copies of yourself, who are the good incarnation (melancholic), crazy incarnation (sanguine) and pragmatic incarnation (choleric). The character of the protagonist depends on how you've been playing him, but he does start out as phlegmatic.
The Major Circuit in the same game have; Piston Hondo (choleric), Bear Hugger (sanguine), Great Tiger (phlegmatic), and Don Flamenco (melancholic).
And the World Circuit have; Bald Bull and Aran Ryan (choleric), Soda Popinski (phlegmatic), Super Macho Man (sanguine), Mr. Sandman (melancholic), and Donkey Kong (eclectic).
Also, in "Super Punch Out", the Special Circuit follows this trope, albeit a bit less straight; Narcis Prince (choleric), Hoy Quarlow (phlegmatic), Rick Bruiser (sanguine), and Nick Bruiser (melancholic).
In Puyo Puyo, the Fever era's ARS trionote the three major eras that are defined in the series each have a trio consisting of a female protagonist whose name starts with "A", a rival to them whose name starts with "R", and a male friend whose name starts with "S" + Klug (who frequently hangs out with them) are a spot on example of this.
Amitie - Sanguine: Friendly, cheerful and optimistic, but also scatterbrained and disorganized with her life.
Raffina - Choleric: Short-tempered, rude and very haughty, but also hardworking and determined to prove her worth.
Sig - Phlegmatic: Gentle, quiet and compassionate, but his lack of energy and interest on most things make him come off as lazy and yielding to the will of others.
Klug - Melancholic: A rational and perfectionist genius student who's not afraid of harshly criticizing others, yet becomes easily embarrassed when he's proven wrong or made a fool of, which happens very frequently.
Les Quatre Alices: Alice L'Orange (Melancholic), Alice D (Choleric), Alice Brown (Sanguine), and Alice Kurosawa (Phlegmatic).
The main character and her friends & family fit these categories well:
Sanguine: Rottytops — bright and cheerful, silly and naive at times
Choleric: Sky — loyal and brave, yet not as tolerant as her friends
Melancholic: Bolo — unlucky most of the time, but more capable than he lets on
Phlegmatic: Mimic — a more reserved and calmer Big Fun; kind and supportive, but usually plays a passive role
Eclectic: Shantae herself — more reserved than Rotty, but more cheerful and outgoing compared to Sky or Bolo
In addition, the fellow Half Genies from Shantae and the Seven Sirens have their own take on the temperaments:
Sanguine: Vera — a kindhearted soul who specializes in healing and refreshing organic life
Choleric: Zapple — strong willed, takes trust and loyalty very seriously, and specializes in electricity
Melancholic: Harmony — the oldest Half Genie and the Cool Big Sis of the group, intelligent, resourceful, and specializes in pyrokinetics and earthquakes
Phlegmatic: Plink — the most quiet and a bit shy compared to everyone else and specializes in finding hidden and invisible objects
Eclectic: Shantae, once again — when grouped with the other four, she shows the most balanced temperament, being more open than Zapple and more outgoing than Plink, but willing to learn from Veras wholesome nature and Harmonys life experience
Sonic (Choleric): The leader of the gang. Driven by his own strong sense of justice, he can be very impulsive and most likely to take action should a problem present itself. He's quick-witted, easy-going and loyal to his friends. To his rivals and enemies, he can be an extremely condescending smart aleck. He has his moments of being arrogant and narcissistic, but is nonetheless a cool individual whos always there to save the day.
Tails (Phlegmatic): The technician of the gang, his intelligence rivals that of Dr. Eggman's. He's very friendly, upbeat and humble, but can be very touchy and prone to self-doubt at times. He can also be naive and a blabber-mouth, which can greatly irritate his friends. Nonetheless, he's driven to chart the course and guide his friends.
Knuckles (Melancholic): The muscle of the gang and Sonic's former rival. He's stubborn, very short-tempered, and terribly single-minded. He's a driven individual who takes his duties as protector of Angel Island, along with the Master Emerald, very seriously. Only problem is that he can be very gullible, naive and easily misled to the point that cunning individuals (like Dr. Eggman and Rouge) can easily get one over on him.
Amy (Sanguine): The leader of her own group (Team Rose) but sometimes resorts to going along with Sonic and his crew. Shes a cheerful and optimistic individual who can be very compassionate and overly concerned with other people's problems. However, she can be very aggressive, stubborn and temperamental. Because of those traits, she can come across as very overbearing and a nuisance sometimes (especially to Sonic).
Sheila: An easy-going, phlegmatic Action Girl from the alps.
Sgt. Byrd: The choleric Rebel Leader of the Hummingbird Resistance Movement against the Sorceress.
Bentley: The melancholic older brother to Bartholomew, with a love of serenity and the fine arts.
Agent 9: An unhinged, sanguine Blood Knight who loves battling Rhynocs and cracking jokes.
Star Wars: Republic Commando: The four Clone Commandos of Delta Squad are this. Delta-38, "Boss", is choleric, showing good leadership instincts. Delta-07, "Sev", is melancholic, being the Cold Sniper of the group and showing great care to place each round for maximum effenciency. Delta-40, "Fixer", is phlegmatic, being, as Taun We put it, "A pure and uncomplicated soldier." Delta-62, "Scorch", is sanguine, showing much enjoyment in blowing the CIS forces apart.
Mario: Sanguine - the cheerful and humble, but overly confident average joe.
Luigi: Phlegmatic - the patient and brainy brother.
Wario: Choleric - the loud and overly emotional hothead.
Waluigi: Melancholic - the neurotic and snarky prankster.
The four prominent women also fit the bill:
Peach: Phlegmatic - the refined and dainty princess.
Daisy: Sanguine - the joyful, excitable tomboy.
Rosalina: Melancholic - the most prominent mother figure.
Pauline: Choleric - the adept, business-minded mayor.
Some of Marios friends and acquaintances qualify as well:
Yoshi: Sanguine - Marios cheery, loyal friend; often described as an embodiment of Kindness.
Toad: Phlegmatic - Peachs friendly and amicable steward.
Donkey Kong: Melancholic - Marios former rival turned ally to the heroes.
Birdo: Choleric - the passionate wild card whose loyalty lies with whoever captures her heart.
The ATX Team in Super Robot Wars. At first the lineup is Sanger (choleric), Bullet (melancholic), Kyosuke (phlegmatic), and Excellen (sanguine). Later, Kusuha was drafted into the team as the eclectic. Later on, Sanger leaves, and Kyosuke takes on the choleric mantle; and when Lamia enters, she takes on the phlegmatic mantle.
The royal family can count as well: the King Asgore Dreemurr and the former Queen, Toriel are both on the line between melancholic and phlegmatic, the Fallen Prince Asriel was choleric, and the First Human appears to be sanguine.
The main characters of Valkyria Chronicles 4 fit this dynamic. Claude is melancholic, Riley is choleric, Raz is sanguine, and Kai is phlegmatic.
Johnathan Reid (Melancholic): The Doctor-turned-Vampire, determined to solve the mystery of the Vampire Epidemic that subsequently ruined his life. He's very much inquisitive and blunt in asking questions.
Edgar Swansea (Sanguine): Leader of Pembroke Hospital, who despite his great appreciation for Johnathan's work. Made many serious mistakes, in an attempt to cure the Spanish Flu. He's naive and reckless in his medical procedures, but does so only for the greater good.
The Red Queen (Choleric): Big Bad and main instigator of the Vampire Epidemic. A vengeful, wicked spirit, who would exterminate all mortals in England, and replace them with vampires. She was responsible, for many real historical tragedies that befallen the world such as the Black Death. Through blood she is awaken, but also is defeated by it.
The Four Horsemen, both versions, in World of Warcraft are this as well. Though each version plays the tropes a little differently.
Sanguine: Thane Korth'azz. He has a penchant for colorful insults and dark humor, while the other characters are much more classically brooding. Also, he has fire powers.
Phlegmatic: Alexandros Mograine. The most level-headed of the group.
Melancholic: Sir Zeliek. Actually he's straight up suicidal, but that's understandable enough under the circumstances. The others don't feel the same way only because they're pretty much insane.
Choleric: Lady Blaumeux. She more eagerly embraces her role as a champion of the Scourge than the others. Even in life it seemed she had a bit of a sadistic streak.
Sanguine: Nazgrim. Retains his honorable and generally easy-going personality, also enjoys his new abilities more than the others. Like most orcs, he has a penchant for crude humor.
Phlegmatic: Darion Mograine. He shares his father's level head though he's a bit more of a .
Melancholic: Sally Whitemane. When she is raised she realizes that she and the Scarlet Crusade screwed up really really bad. She sees serving the Ebon Blade as a kind of weird and twisted redemption.
Choleric: Thoras Trollbane. In life, this mainly manifested as extreme racism (he left the Alliance because they refused to execute their orc POWs). In death, he's understandably pretty mad about what happened to his Kingdom and about being killed by his son Galen. His sword Trol'kalar is an infamous artifact of a genocidal campaign, from whence the family got their name "Trollbane".
Their respective creators, Kel'thuzad and the Lich King, both qualify as Eclectic. They're both a bit all over the place: though Kel'thuzad is more Phlegmatic while the Lich King is more Sanguine. Incidentally, this trope is further evidence that the Lich King never intended Tirion Fordring to be in the group. Tirion's personality was Choleric: idealistic, brash and vengeful.
Xenogears has the Neo-Elements: Seraphita (sanguine), Tolone (choleric), Dominia (melancholic), Kelvena (phlegmatic). The characters are also associated with the classical elements, but not quite the same ones traditionally associated with the temperaments; Dominia and Kelvena match up (earth and water, respectively), but Seraphita and Tolone are switched (the sanguine temperament is associated with air and the choleric with fire, but Seraphita is associated with fire and Tolone with air).
In Yandere Simulator, the members of the Student Council currently implemented in the game fit this: Kuroko (Melancholic - the most reponsible one and formal at all times), Akane (Sanguine - friendly, gentle and ditzy... or not), Aoi (Choleric - prefers to use intimidation and violence to achieve her goals) and Shiromi (Phlegmatic - eccentric, perpetually calm and always smiling).