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  • The four protagonists of The Anthem of the Heart, which is even lampshaded within the film. Natsuki is sanguine. She's the friendly, hardworking cheer captain of the team. She's accommodating, but can give in to peer pressure. Daiki is choleric. He's a passionate and hardworking leader but can be demanding and rude. Takumi is melancholic. He's introverted, avoids talking about his feelings, and is seemingly apathetic, but is considerate, decisive, and sensitive. Jun is phlegmatic. She's self-deprecating and passive to the point of having debilitating anxiety, but is compassionate and kind.
  • Bakugan:
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    • The Team in Season 1:
      • Dan and Runo (Choleric) are hot-blooded, driven and always up for a fight. They also end up together.
      • Marucho and Julie (Sanguine) are cheerful and optimistic.
      • Shun (Melancholic) is withdrawn, quiet and isolated.
      • Alice (Phlegmatic) is mature, compassionate, and soothing.
  • Baka and Test: Summon the Beasts:
    • Boys: Akihisa Yoshii (sanguine; a friendly ditz), Yuuji Sakamoto (choleric; the leader of the boys and a plan-maker who's determined to climb the school ladder), Hideyoshi Kinoshita (phlegmatic; usually keeps his cool and is the most sensible of his classmates), and Kouta Tsuchiya (melancholic; taciturn and untalkative, only cares about taking perverted pictures).
    • Girls: Minami Shimada (choleric; she's an easily provoked Tsundere), Mizuki Himeji (phlegmatic; gentle and friendly), Shouko Kirishima (melancholic; a quiet Yandere completely focused on the object of her affections), and Aiko Kudou (sanguine; friendly, easy-going and mischievous).
  • Although everyone in Big Windup! gets his/her turn to shine, the story tends to focus on four core members of the baseball team:
  • Black Lagoon:
    • Sociopathic Hero Revy (very choleric), Wide-Eyed Idealist Rock (melancholic), Bald, Black Leader Guy Dutch (phlegmatic), and Mission Control Benny (sanguine).
    • The four most feared women within the series:
      • Revy (Choleric): Easily the most vile and foul-mouthed of the four, disregarding anybody (and anything) that dares to piss her off.
      • Balalaika (Melancholic): Analytical and resourceful when getting information, rather its through her men or destructive means.
      • Roberta (Phlegmatic): A seemingly humble and gentle maid, who disguises her unstoppable killer intent with conviction and modesty.
      • Shenhua (Sanguine): Chatty and openly sociable to everyone, deadly in a glamourous way with a hint of Oriental charm.
  • The original Bubblegum Crisis:
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    • Choleric: Hot-Blooded badass Priss.
    • Melancholic: Stoic leader Sylia.
    • Phlegmatic: Linna the average Jane.
    • Sanguine: Perky and ditzy Nene.
  • Chrono Crusade:
    • Sanguine: Rosette (Hot-Blooded, cares a lot about others but has a violent temper).
    • Choleric: Satella (prideful, determined, and focused on her revenge).
    • Melancholic: Azmaria (a prodigy singer, very caring about others, but insecure and hard on herself).
    • Phlegmatic: Chrono (compassionate and nurturing, but easily discouraged)
  • Death Note:
    • The main cast:
      • Light, who is concerned with saving the world from criminals by killing them and develops a god complex (choleric)
      • L, who always tries to solve cases and rarely sleeps (melancholic)
      • Misa, an energetic girl who is obsessed with Light (sanguine)
      • Ryuk, who doesn't care for anyone and has no real goal (eclectic)
      • Rem, who is like a mother for Misa and would do anything for her (phlegmatic).
    • Also, the police officers:
      • Soichiro, who always works and is the most concerned with the case (melancholic)
      • Matsuda, the youngest and most childish member (sanguine)
      • Aizawa, who can get angry pretty easily (choleric)
      • Mogi, who talks the least and is the most calm (phlegmatic).
  • The Digimon series
  • Dragon Ball Z:
    • Goku (sanguine), Vegeta (choleric), Piccolo (melancholic), Gohan (phlegmatic)
  • In Endride, the central four Ignauts demonstrate these traits:
  • Fist of the North Star has the four Hokuto brothers: Raoh (Sanguine), Jagi (Choleric), Kenshiro (Melancholic), and Toki (Phlegmatic).
  • Hellsing has three:
    • The Hellsing Organization: Alucard (choleric), Seras (phlegmatic), Walter (melancholic), Pip (sanguine) and Integra (leukine).
    • The Iscariot Organization: Alexander Anderson (sanguine), Heinkel Wolfe (choleric), Yumie Takagi (phlegmatic), and Enrico Maxwell (melancholic).
    • Millennium: The Major (sanguine), The Captain (melancholic), Doctor (phlegmatic) and Schrodinger (choleric).
  • Jewelpet:
    • Jewelpet Happiness has the boys quartet: Kousuke (phlegmatic; the gentle and low-key guy who's a little spacey but always ready to give advice), Takumi (melancholic; the academically accomplished cold perfectionist), Mouri (sanguine; the most openly friendly and sociable of the boys), and Taira (choleric; the aggressive but insecure guy who always feels he has something to prove).
    • Lady Jewelpet has two sets:
      • The Petit Ladies are Momona (sanguine; open, friendly and cheerful, if an easily distracted klutz), Lillian (melancholic; cold, initially friendless and focused on her goal of becoming the best Lady), Mizuki (choleric; practical, blunt and easily exasperated) and Charon (phlegmatic; a shy bookworm and a Nice Girl); the Sixth Ranger Elena is leukine (for embodying everything considered essential in a Lady).
      • The Princes are Cayenne (leukine; well-balanced personality among the rest of the boys), Romeo (phlegmatic; a real gentleman, open-minded and considerate), Soarer (melancholic; intelligent and methodical, notably dislikes it when things don't go by the book) and Levin (sanguine; impulsive Large Ham and a Nice Guy); the Sixth Ranger Miura is choleric (as the cynical and mocking resident bad boy).
  • JoJo's Bizarre Adventure:
    • The main characters of Stardust Crusaders:
      • Joseph Joestar: The eclectic. Has mellowed out considerably since Battle Tendency, making him more balanced in comparison to his grandson and the rest of the group.
      • Jotaro Kujo: The choleric. The unemotional, violent delinquent.
      • Noriaki Kakyoin: The phlegmatic. The moral, polite heart of the five.
      • Mohammad Avdol: The melancholic. Outwardly stoic, but is actually capable of displaying emotion and even has a bit of a temper.
      • Jean Pierre Polnareff: The sanguine. The chivalrous, arrogant Emotional Bruiser.
    • The Jojos of the first five parts:
      • Joseph Joestar: The sanguine. Impulsive, prideful, and teasing.
      • Jotaro Kujo: The choleric. Aggressive, intelligent, and practical.
      • Giorno Giovanna: The melancholic. Reserved, strong-willed, and compassionate.
      • Josuke Higashikata: The phlegmatic. Laid-back, Adorkable, and kindhearted.
      • Jonathan Joestar: The eclectic. Is both gentle and confident. As such, he's much more well-rounded compared to his descendants.
  • K-On!'s original quartet of protagonists:
    • Sanguine: Yui, the ditzy Nice Girl who wants nothing more than to have fun.
    • Choleric: Ritsu, the brash Genki Girl and nominal leader of the group, is a bit of a bully but ultimately wants to make her friends happy.
    • Melancholic: Mio, The Reliable One who tries to be mature and act as the voice of reason, but is extremely sensitive and introverted.
    • Phlegmatic: Mugi, The Quiet One but also a Nice Girl like Yui, who provides as much as she can for her friends.
  • Kara no Kyoukai
    • Melancholic: Shiki; emotionally detached, cynical, and standoffish.
    • Phlegmatic: Mikiya; friendly, passive, and loyal.
    • Choleric: Touko; very morbid, cold, and suffers from an extreme case of apathy.
    • Sanguine: Azaka; cheerful, passionate, and energetic.
  • Kill la Kill has three groups:
    • Main Characters:
    • The Elite Four:
      • Sanguine: Sanageyama — Fight-happy swordsman interested only in finding opponents that can challenge him.
      • Melancholic: Gamagoori — Firm disciplinary officer and enforcer of Satsuki's rules, and the most overt in loyalty towards her.
      • Phlegmatic: Inumuta — Preferring to gather and study as much data as possible rather than take an active field role like the others.
      • Choleric: Jakuzure — A driest sense of humor. She also takes great pride in the fact that she's known Satsuki the longest.
    • The Kiryuin Conglomerate:
      • Melancholic: Ragyo — The Big Bad of the series and an all-around pompous character whenever she gets her moments. She showcases the negatives more than the positive features, had abnormally high wants when she was testing her own daughters, but when they do fulfill them, she is filled with glee.
      • Choleric: Nui — Arguably Co-Dragons with Rei to Ragyo, and the one who gets the most work done initially while Ragyo sits around and controls REVOCS. Showcases the downsides by being outwardly nice, extremely crazy inside, and even more insane after normal Ryuko cuts off her arms.
      • Phlegmatic: Rei — The person able to serve at a moment's notice, and without question. However, she showcases the downsides by being an outwardly cold person. Exemplifies this part of her personality in the OVA by being a Defrosting Ice Queen when she heard of Ragyo's death, and was able to be swayed by Satsuki.
      • Sanguine: Mind-Controlled Ryuko — Instead of unable to express her emotions, she says them bluntly, taking them (sometimes literally) to the person she speaks to. Ryuko even kisses Nui, showcasing their love for each other. She balances out the other's negative emotions by being compassionate with them and follows after Ragyo by being way over-the-top in battles, but she showcases the negatives by being mind-controlled into finally finding her wants of being with an actual family and comes off as harsh to others.
  • The Legend of Zelda: The flash animations by Hadouken Dude took this to absolutely hilarious levels.
    • Choleric: Red Link being the self-centered, temperamental and trouble-making Jerkass.
    • Melancholic: Green Link being the fearless, determined and uptight leader.
    • Phlegmatic: Blue Link being the analytical, cautious, and mildly snarky intellectual.
    • Sanguine: Orchid Link being the childish, mischievous and clumsy noob.
  • The main characters of Little Witch Academia:
    • Sanguine: Atsuko "Akko" Kagari. The optimistic, temperamental Genki Girl.
    • Phlegmatic: Lotte Jansson. The levelheaded, albeit shy, Nice Girl.
    • Melancholic: Sucy Manbavaran. The aloof, apathetic Deadpan Snarker.
    • Choleric: Diana Cavendish. The blunt, confident Jerk with a Heart of Gold.
    • Eclectic: Ursula Callistis/Chariot du Nord. The calm, yet charismatic, Badass Teacher.
  • The Hinata House Girls in Love Hina:
    • Naru Narusegawa & Motoko Aoyama (choleric), Mitsune Konno (melancholic), Kaolla Su (sanguine), Shinobu Maehara (phlegmatic), and Mutsumi Otohime (eclectic).
  • Macross Delta
    • Delta Squadron:
      • Eclectic: Hayate Immelman. Brash, carefree rookie.
      • Sanguine: Chuck Mustang. Sociable, the "mood maker" of Delta.
      • Choleric: Mirage Farina Jenius. Mission focused, has a volatile attitude.
      • Melancholic: Messer Ihlefeld. The ace, has a serious demeanor.
      • Phlegmatic: Arad Mölders. The oldest member of the team as well as the leader of Delta.
    • Walküre:
      • Eclectic: Freyja Wion. New member of the team, has a great love for singing
      • Sanguine: Makina Nakajima. Bubbly, carries an air of happiness around her.
      • Choleric: Mikumo Guynemer. The most action-oriented member of the team.
      • Melancholic: Reina Prowler. Stoic, quiet, close-minded.
      • Phlegmatic: Kaname Buccaneer. The leader, acts as the manages schedules and mission briefings.
  • Magical Girl Lyrical Nanoha Strikers. natural leader (former) Tsundere Teana (choleric), knight-in-training Erio (melancholic), Caro (phlegmatic), and Genki Girl Pollyanna Subaru (sanguine). The last two's personalities actually caused a bit of family drama for their also melancholic adopted mother in the second StrikerS Sound Stage, since their combined introvertedness caused some communication problems.
  • Miss Kobayashi's Dragon Maid: Kobayashi and the three main dragons.
  • The main male characters from My Hero Academia fit this perfectly:
    • Phlegmatic: Izuku Midoriya. Shy, altruistic, and cripplingly insecure.
    • Choleric: Katsuki Bakugo. Vicious, prideful, and extremely antagonistic.
    • Melancholic: Tenya Iida. Disciplined, modest, and has a strong sense of justice.
    • Sanguine: Eijiro Kirishima. Kind, confident, and Hot-Blooded.
    • Eclectic: Shoto Todoroki. Acts both unemotional and emotional, making him the most balanced of the five.
  • Puella Magi Madoka Magica: The Magical Girls.
    • Sanguine: Sayaka Miki. Idealistic, courageous, and compassionate.
    • Choleric: Kyoko Sakura. Callous, passionate, and stubborn.
    • Melancholic: Homura Akemi. Intelligent, aloof, and very goal-oriented.
    • Phlegmatic: Madoka Kaname. Kind, shy, and insecure.
    • Eclectic: Mami Tomoe. A balanced mentor to the other girls.
  • The Quintessential Quintuplets: Yotsuba (sanguine), Nino (choleric), Itsuki (eclectic), Miku (melancholic), and Ichika (phlegmatic).
  • The current lineup of the Taishiro in Samurai Deeper Kyo fits almost flawlessly.
    • Sanguine: Yuan is extroverted, fond of wisecracks, and very protective of the people he cares about. He uses fire techniques and wears red.
    • Choleric: Tokito is quick to anger, perfectionistic, and impulsive. Wears shades of yellow, and fights with a very aggressive energy.
    • Melancholic: Hishigi is exceedingly introverted and the epitome of The Stoic, analytical and extremely task-focused. He shows textbook signs of severe depression. His color scheme is almost entirely black.
    • Phlegmatic: Fubuki leads the Taishiro. He is level-headed, cultured and elegant. His fighting style revolves around water and his color scheme is mainly blue.
  • Superbook (second season): Christopher Peepers (choleric), Joy (melancholic), Uriah (sanguine), and Gizmo (phlegmatic).
  • The four main characters from Texhnolyze: Doc (sanguine), Onishi (choleric), Ichise (phlegmatic), and Ran (melancholic).
  • The Royal Tutor:
    • The four main princes: Kai (phlegmatic), Bruno (melancholic), Leonhard (choleric), and Licht (sanguine).
    • Heine and the other members of the Granzreich royal family:
      • Phlegmatic: Heine. Stoic, intelligent, modest, and patient... as long as no one comments on his size.
      • Melancholic: Viktor. Stern, rational, and cares strongly for his children.
      • Sanguine: Adele. Happy-go-lucky, childish, and playful.
      • Choleric: Eins. Unemotional, confident, and condescending.
      • Eclectic: Beatrix. The well-rounded Cool Big Sis to the four brothers.
  • Five-temperament ensemble in Tokyo Mew Mew: At first not happy about leading, but accepting her responsibility later on, Ichigo (choleric); Minto (melancholic), Retasu (phlegmatic), the Genki Girl Bu-ling (sanguine), and Zakuro (eclectic).
  • The Tower of Druaga:
    • Jil's Group: Jil (melancholic), Ahmey (phlegmatic), Melt (choleric), Coopa (sanguine), and Kaaya (eclectic).
    • Neeba's Group: Neeba (melancholic), Fatina (choleric), Kally (phlegmatic), and Utu (sanguine).
  • Trigun more often averts this trope in favor of the Lone Hero or the Power Trio. Adding Wolfwood to the cast has a subtractive effect instead, generating a duo dynamic that leaves Meryl and Millie on the sidelines. When they participate equally they fit the four temperaments: Meryl (choleric), Vash (melancholic, though pretending to be sanguine), Wolfwood (phlegmatic), and Millie (sanguine).
  • In the original Weiß Kreuz Aya (choleric), Omi (melancholic), Youji (phlegmatic), and Ken (sanguine).
  • In Wolf's Rain: Tsume (choleric), Kiba (melancholic), Toboe (phlegmatic), and Hige (sanguine).
  • Your Lie in April: Arima Kousei (melancholic), Miyazono Kaori (choleric), Watari Ryota (sanguine), Sawabe Tsubaki (phlegmatic).
  • Yu-Gi-Oh! 5D's: Jack (choleric), Yusei (melancholic), Akiza (phlegmatic), and Crow (sanguine).
  • Out of the four "Yu's" from Yugioh Arc V:
    • Sanguine: Yuya aspires to make people smile and peacefully solve conflicts but can be emotionally unstable.
    • Choleric: Yugo is hot-tempered and occasionally impulsive but is ambitious and protective of loved ones.
    • Melancholic: Yuto is determined, pragmatic, and stoic, but has a self-blaming and cynical streak.
    • Phlegmatic: Yuri is always level-headed and calm, even when relentlessly hunting people under orders of his leader, or being a comedic plaything.

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