Darths & Droids: Pete views killing NPCs as a chance to grab bonus XP, but even he finds Annie's sheer glee in killing them during the "Revenge of the Sith" intro to be unnerving, and views the plan to use their half-dreadnought's fuel and all its missiles as a braking method to be too insane even for them.
Porn star Bimbo of Exploitation Now has slightly more morals than Ralph when it comes to a sexual lifestyle. She finds his excessive nightly masturbation sickening and feels there's "something wrong" with watching porn while eating breakfast.
One of the tentacle monsters in Ghastly's Ghastly Comic thinks it's wrong to sexually assault human women, although this is at least in part due to a personal distaste for them.
A meta example for Homestuck. Hussie intentionally put the webcomic on hiatus before Act 6 Act 6 Intermission 3 due to the events that transpired during it. He admits in the newspost after he put the update up that even he isn't that cruel.
Ménage à 3 draws most of its humor from the fact that most of the cast are self-absorbed and sex-mad. However, they almost all retain some sympathetic features, and it's clear that they have lines they won't cross. For example:
Zii objects to Gary attempting to "pimp her out" to Amber (it's a long story); she may be very sexually active and broad-minded, but it's a question of consent. Some readers have suggested that her standards there are a little ambiguous, given her own past behavior, but they do exist.
DiDi, whose capacity for empathy is questionable and whose quest for an orgasm is dangerously obsessive, becomes wildly sentimental about any kind of sob story, and makes some effort to avoid hooking up with people who are already in serious relationships.
Amber may trick Gary into giving her oral sex, and use her sexuality to scam herself a free apartment, but she's a nice person really, just very uninhibited; the idea of someone manipulatively withholding sex disgusts her.
Matt may be a hopelessly vain jerkassCasanova, but he's reliably decent to his friends and treats Ruby (from the spinoff Sticky Dilly Buns) notably kindly during their one brief meeting.
And even total jerkass Nathan has some lines he won't cross — for example, he can see that actually realising his twincest fantasy would probably be traumatic for the twins.
In Motherly Scootaloo, Jet Set and Upper Crust may be jerks, but the only reason they accused Scootaloo of murder in the Trial was because their attorney had assured then Scootaloo was 100% guilty.
In The Order of the Stick, Roy is surprised when Hayley is on-board with a plan to stop and help some poor villagers who couldn't realistically pay them anything in return. She actually gets annoyed by this, noting that just because she loves treasure doesn't mean that she's opposed to doing anything for free.
Comes up in the Penny Arcade comic "We Do In Fact Have Limits", where Gabe suggests to Tycho that they could do a strip about spawn camping where a terrorist waits for a woman to give birth just so he could kill the baby. Tycho is less than thrilled with this suggestion and grabs Gabe by his shirt while giving his honest opinion on the idea.
Tycho: No, we can't ever do that. No-one can even know that you said it, or that I heard it. It will be our secret, forever.
Tagon of Schlock Mercenary has few moral standards beyond getting paid, but while he may not be a particularly good person, he refuses to be a bad commander and is A Father to His Men. In the original charter for the Toughs, there's a note that death benefits for both enlisted troops and officers come out of the captain's dividends, to ensure that lives are spent responsibly.
In Sonic the Comic Online!, even Sonic is pretty disgusted when he gets a chance to see just how much of an ass he was in the past.
Stand Still, Stay Silent: In the prologue, Michael Madsen's boss threatens to fire him if he doesn't come back from his personal ferry trip to Bornholm island by the next day. Because of this, Michael suggests various solutions, including taking a night ferry. His boss, despite his insistence on having him back as soon as possible, hesitates about that option as it sounds like "something smelly" to him, and suggests seeing if he can hire a helicopter instead.
Parodied in this page of xkcd, where the Black Hat Guy's standards are rather arbitrary.
Black Hat Guy: I plead the third. Congressman: You mean the fifth? Black Hat Guy: No, the third. Congressman: You refuse to quarter troops in your house? Black Hat Guy: I have few principles, but I stick to them.