Hild can cow even first-class goddesses with one meaningful look. Seemingly hereditary as her half-goddess/half-demon daughter Urd seems to be able to do it as well.
Yamato Nadeshiko postergirl Belldandy has a chilling one ready in the rare case someone is seriously abusing one of the people close to her. You need to try really hard to get her to make one though. When she thinks her Father is toying with Keichi about him passing or failing the final judgement about their ability to be together, she glares and the avatar of his form sweats and hides near Keichi.
And Yet the Town Moves. Hotori insists that Kon add ridiculous ingredients to her cooking. Kon snaps, prompting a terrified Hotori to stand down.
Assassination Classroom: Nagisa can pull off some truly chilling ones when he gets pushed, which is even more surprising considering how kind a boy he usually is. What makes it worse is that he's often smiling serenely as he does so. His death stares are so effective that one actually drives a would-be murderer clinically insane from sheer terror, desperately screaming for Nagisa to never look at him like that again.
Eren has an especially creepy one◊ as a young child. He pulls this face before killing two grown men with a knife to save a girl from being kidnapped.
Hurt or threaten Eren in any way, and Mikasa will give you a terrifying one. Levi must really be Humanity's Strongest soldier, if he survived this◊, and Bertholt in turn earns his status as the Colossal Titan when he somehow survives this, though he did flinch from it - but who could blame him?
This appears to be Reiner's default reaction to an attack by a human. Right before transforming into the Armored Titan, Mikasa attempted to assassinate him along with Berholdt, and both Mikasa and Reiner exchanged glares when this occured. He also reacted like this against Levi, who of all people was disturbed.
Baki from Baki the Grappler is a master of intimidating Death Glares. He can throw one even while kissing (and without letting his girlfriend notice that something is not right).
Gennosuke and Hyoma from Basilisk take this trope a little too literally, as they have to power to look at people and turn their killing intent against them, causing them to commit suicide on the spot. Naturally, using this power causes them to glare angrily at their victims while they're at it.
Theresia, a girl of about 12 years old, gives one of the scariest death glares◊ of all time to Guts. He is unaffected since he was deliberately trying to invoke this trope to keep Theresia from committing suicide. And given what she's just gone through prior to this, one cannot blame the girl at all.
Played for Laughs with Captain Unohana, whose Yamato Nadeshiko personality means her disapproving expression doesn't even look scary— and yet it instantly subdues even those who aren't normally scared of anything, such as members of the 11th division or Ichigo. Justified because in her past, she was the most dreaded Blood Knight in the Soul Society's history.
Yamamoto semi-adopts this as his default expression after his lieutenant's death. In his fight against the fake Juha Bach, he whips out one that is about everything a Death Glare should be. He's clad in his Bankai's reiatsu which radiate a 15 million degree heat, his blade obliterates anything that touches the blade's edge, can summon the corpses of those he's killed, and can discharge the full power of his Bankai into one compressed blast. And then he puts on this expression. At this point, normal people would be beyond relieving their bowels and would be shrieking for any shred of conceivable mercy from Yamamoto.
In The Circumstances Leading to Waltraute's Marriage, the Valkyrie Waltraute has a glare that frightens anybody who sees it, even Odin. When she meets her future husband Jack Elvan, the first thing that impresses her is that he is completely unaffected by it.
In Digimon Data Squad, ShineGreymon gives Kurata one after assuming Burst Mode. It's filled with so much hate that you can practically hear ShineGreymon saying "I'm going to kill you". The Unflinching Walk as he delivers the Death Glare combined with Kurata's Oh, Crap! reaction to this only adds to it.
A Super Saiyan is prone to give quite the death glare when pushed too far. Especially Goku and his eldest son Gohan. Actually, their expression seems locked into this when they use it during a fight. The only time when a Super Saiyan is seen using other expressions while in its mode is while Goku and Gohan are living in their Super Saiyan forms to increase their power.
Gohan's ultimate form in his fight with Buu is made of death glare, as part of his anger fueled power-up he always looks pissed in this form. Piccolo even commented about it.
The Super Saiyan Three Death Glare is worse, because Goku looks truly murderous when transformed, and there's no hint of humanity behind it, as he looks like a primitive, wild hedgehog/human hybrid. Gohan's Super Saiyan II transformation has a similar effect, same as Goku's when he fought Majin Vegeta. Not having eyebrows makes it all the more effective.
Vegeta's eyes are permanently locked into this expression. No matter how wide he opens his eyes, his eyebrows don't lift and the furrow above his nose never relaxes.
And then there's Mastered Ultra Instinct Goku's murderous glare given to Jiren in the penultimate episode of Dragon Ball Super.
Better not threaten to hurt Winry in front of Ed. You try and harm his automail mechanic, and you'll need an automail mechanic!
Also Ed and Al's teacher. Just her normal gaze is enough to make them Blue with Shock, and her actual death gaze, well...
And don't get on the bad side of Alex Louis Armstrong. If he looks at you that way, it's already too late. Enjoy the beating.
Though he is nothing compared to his sister, GeneralOliviaArmstrong. Her normal look has shades of this. When she gets angry, well... let's just say that she doesn't need her sword to kill you.
If you harm one of Roy Mustang's subordinates, or worse, friends (though they are often the same), prepare for the death glare to end all death glares... if you can even see it while burning alive. Envy learned this the hard way.
Aside from Rose, any character showing up for more than two episodes provides various examples.
Shiina of Gourmet Girl Graffiti does this towards a random couple leaving the train station at the end of Episode 5 after having eaten too much of Ryou's food and feeling a little sick from it. As they talk about what to eat that night, she doesn't seem too comfortable listening to it and glances their way with this look.
In Haganai, Kodaka has the Face of a Thug, so sometimes he inadvertently does this to people, such as when he first arrives to class late and exhausted, and in Episode 2, when he asks 2 classmates who are playing a game he's playing if they'd be willing to trade items with him. He does occasionally utilize this trope intentionally too, such as in Episode 3 when Sena is getting hit on by 3 guys and is clearly uncomfortable around them.
In Haikyuu!! manga, Karasuno's captain, Sawamura, has a death glare that's really scary. It might be a sports-focused manga, but that doesn't mean you can't have a nice character with a frightening trait.
An episode in the first season of Hell Girl, Villain of the Week Gil de l'Enfer on the receiving end of one from the normally extremelystoicAi Enma herself. It's the most emotion she ever shows until the last three episodes. Gil had been bragging about how he took bloody revenge from beyond the grave after he was killed by his family for having supernatural powers. A later episode revealed that Ai did the exact same thing to her village when the villagers used her and her family for Human Sacrifice and the second season reveals the her being the Hell Girl is actually an Ironic Hell for having done so, so she had a pretty good reason to be angry.
Also by Yoshihiro Togashi, Gon from Hunter × Hunter can give one of the scariest death glares in anime history if you manage to piss him off. To elaborate, between Gon and Killua, the latter is more than likely to give a murderous Kubrick Stare. All the same, you'd expect that from an assassin. However, Gon is usually a very cheerful kid. But then, he'll get pissed off and his Death Glare ends up looking more intimidating then Killua's! It's very jarring seeing his bright eyes become completely glintless and darken as he practically stares through his enemies.
Inuyasha: Sesshomaru is good at this. He can scare away a ravening pack of youkai-wolves with a glare and once used it to terrify Kagome and Shippou into silence when he overheard them discussing healing his wounds.
Legend of Galactic Heroes has a serious clash between two military troops stopped by a single Death Glare from Admiral Wahlen. Reinhard von Lohengramm is a master of this, too.
In The Love And Creed Of Sae Maki, Sae gives Ran an increasingly deranged series of stares during their meeting at the karaoke club; getting more intense as each excuse Sae offers is easily swatted away and Ran worms her way deeper into her Destructive Romance with Misao . . . until Sae goes to the bathroom for a moment and returns with a welcoming smile and accepts Ran into their group, very clearly Not Herself.
Vita of Magical Girl Lyrical Nanoha A's is usually glaring angrily during combat. Those are all trumped by the genuinely murderous look she gives Nanoha (who's frozen in terror) during a surprise hospital visit to Hayate...
Judau Ashta of Mobile Suit Gundam ZZ throws one of these at Haman Kahn after Haman shoots his little sister. This is notably the first time in the series we actually see real hatred in Judau's face, and this, backed up by his raw Newtype powers, causes the normally Iron Lady Haman to break down and flee in sheer terror, while every Newtype around for miles suddenly feels Judau's intense fury.
In Monthly Girls' Nozaki-kun, Wakamatsu unintentionally gives a frightening glare to Ryousuke when he's approached by him, thinking Ryousuke is going to threaten him. Ryousuke in return is terrified by these "eyes of hatred" and wonders what on earth his sister Yuzuki did to him.
Shota Aizawa in My Hero Academia has this as his Quirk. Nullifying or "Erasing" the quirk of someone else by keeping a constant unbroken gaze on them. With his eyes glowing red, and his hair raising up ominously.
Captain Yamato often gives one of these whenever Naruto and Sai are arguing.
During his earlier appearances, it was usually mentioned that Gaara seemed to have this expression all the time. Complete with a horrific Slasher Smile. The slasher smile is under his mask of sand that he wears during his fight with Rock Lee.
If they are not frowning, Sasuke and Neji are giving someone the death glare.
Sasuke, in fact, has been known to hold entire conversations simply by holding a steady death glare and letting the other guy talk. This is amazingly effective, and he has driven opponents to the point of recklessness simply by doing this. It doesn't hurt that he has a literaldeath glare.
In Chapter 502 Minato gives one to Tobi after saving Kushina from the released Kyuubi.
Naruto gives one on Chapter 538, one so damn potent and downright terrifying that even the GODDAMN KYUUBI was frightened.
Chapter 599 reveals that Tobi is in fact Obito Uchiha. Not only is there not a trace of Kakashi's teammate's former personality left, he also glares maniacally throughout Chapter 560 as he rebukes Naruto and Kakashi's comments.
Tobirama and Hashirama give one each in rapid succession in Chapter 620. The former's Berserk Button was pressed when Sasuke threatened Konoha and the latter gave the former a Death Glare to calm him down. These two are the First and Second Hokages. It's no wonder that Orochimaru, Sasuke, Suigetsu and the Third Hokage freaked out.
In The Sunny-Side Battle! OVA as a Played for Laughs moment Itachi gives a death glare to Sasuke when he attempts to pick up one of the plates containing a failed egg Itachi made after he asked Sasuke if he wanted some eggs not realising that Itachi is only going to settle with giving him a flawlessly made egg. Adds frosting on how Itachi had his Sharingan activated.
Deidara gave Tobi one of these on several occasions while partners in the Akatsuki, usually triggered by Tobi doing/saying something stupid or offensive to Deidara's art.
Neon Genesis Evangelion. This trope + Scary Shiny Glasses = Gendou Ikari. Since his wife's death, he appears to be stuck into a permanent death glare he only lets up when he's looking at his virtual daughter Rei.
In One Piece, this actually counts as a superpower that only the strongest pirates can use, called Conqueror's Haki.
Luffy began developing the ability unconsciously. During the timeskip he has fully mastered it, along with the other two types...to the point where he can now use it to knock out 50,000 enemies in three seconds.
Luffy adopted a non-haki version of this in his Tranquil Fury after Charloss shot Hachi. The World Noble was very understandably frightened when he saw Luffy's face and opened fire on him in panic.
Shanks was the first shown user, right in the beginning episodes. He used it to make a giant sea snake (that just bit off his freaking arm) shit itself and run away.
Silvers Rayleigh uses it to make an entire room of attacking guards collapse. He then went on to train Luffy in it's use.
Aside from Haki users, some people use non-superpowered versions:
Roronoa Zoro has been accused on several occasions of having a "scary face". As we can see from this one example◊.
Nami has also made some very scary faces. You do NOT want to make her angry. More often than not, Nami falls under the category of Demon Head.
Boa Hancock gave Jimbei one when he started looking hungrily at a buffet Hancock made for Luffy. He was only allowed a few bites. Though she has not yet been seen using it, she is also said to have the Conqueror's Haki.
Dracule "Hawk eyes" Mihawk. It has been said that the one thing that all his victims remember are his eyes.
Robin gave Sanji a frightening one in Thriller Bark complete with Glowing Eyes of Doom when he started daydreaming about Robin's docking which she had refused to do.
Justified in Parasyte where all animals (including humans) have an "instinct" that warns them when they're facing a stronger enemy. When Shinichi, who is host to a deadly parasite in his arm, glares at you, he can convey "You want me to eat you?" clearly with a single glance.
Grings Kodai from Pokémon: Zoroark: Master of Illusions is such an evil, horrible person that he awakens to find himself on the receiving end of a mass Death Glare from the cast, including two characters who are just now discovering what an evil person he is. The only sounds in the whole scene are his Evil Gloating playing in the background over the TV network.
Misty also tended to be a master of these. Especially when meeting Melody in Pokemon the Movie 2000.
Pikachu gave Meowth a particularly hate-filled one when Meowth revealed himself to be a HeelFace Mole in "Best Wishes!"
Before that, "Dues and Don'ts" had him give one to Team Rocket after he was unintentionally healed by the Delibird kicking its butt minutes before.
James is normally submissive and timid, but when Jessie and Meowth refused to help the heroes save some endangered baby Pokemon, he responded by glaring a hole through them until they agreed to help.
Pokémon the Movie: Black/White has Iris giving Emolga one after the latter used Volt Switch.note A Gen V move where the user electrifies an opponent, using the flash to self-eject and switch places with an allied Pokémon As this is a single-elimination tournament, this got Iris disqualified.
Mewtwo from Pokémon: The First Movie has a bowel-voidingly terrifying one as a default expression, and general rule of thumb is that whoever it's aimed at is screwed.
From the same movie, Ash gives one to Mewtwo as he emerges from a cloud of smoke with the original Pokémon in tow.
In "Journeys", Chloe gives a terrifying one to two women in "The Cuteness Quotient" for insulting Jinny's Feebas and calling it ugly. Cure Mimikru eyes and flaming aura surrounding her.
Oleana gives a menacing one to Ash in "Sword And Shield Slumbering Weald" after he declines Rose's offer.
Corporal Randel Oland from Pumpkin Scissors gets this whenever he goes into his equivalent of "berserk mode".
The Rising of the Shield Hero: Considering everything he went through since his third day, Naofumi was handing these out in spades, especially in the manga. But the crowning one ironically comes in the Lighter and Softer anime when King Aultcray, the one whose partly responsible for everything that went wrong for him had thrown a heavily implied death threat towards Raphtalia and Firo, simply because the former was utterly done with dealing with the unjustified crap constantly being handed to him. Having already been pushed past his breaking point, Naofumi gives the mother of all death glares to the now scared-shitless king, along with swearing to painfully end him if he so much as touches his companions.
Kenshin of Rurouni Kenshin is the absolute master of this. And at one point he faces Kurogasa, who has a literal death stare — by glaring at an opponent with his ki, he paralyses them. It takes a strong-willed person to resist it.
Oda Nobunaga in the anime. He paralyses Masamune to the spot just by staring at him.
This is Ishida Mitsunari's default expression.
Played for Laughs in Slam Dunk. At one point, Sakuragi tries to ask Akagi how to avoid fouling out in every match. Akagi, to get him off his back for the time being, suggests something like "Kill your opponents' moves with your glare". Sakuragi takes it that he means this trope so he decides to try it. It works against his teammates during practice, but in a real match, his opponents aren't so easily cowed.
Lawrence, from Spice and Wolf, in Episode 4 of the second season. While he probably didn't intend to kill the stranger he rudely bumped into, he gave him a glare that made the much taller and tougher-looking guy back off instantly.
In Season 2 of Strike Witches, Eila gives a particularly angry close-up one to Yoshika due to the latter being chosen to protect her partner Sanya in the rocket launch operation instead of her.
In Episode 4, after Ryoko and Ayeka each trade an Armor-Piercing Slap with each other (Ryoko giving two to Ayeka) in the Hot Springs Episode, Tenchi tries to break it up, only to met with a Death Glare from both.
Tenchi: Excuse me, ladies... (Ryoko and Ayeka GLARE) Tenchi: (retreating back behind the rock) Never mind.
This is also what Airi Masaki uses to break the will of RYOKO. Washu states that it is part of the Neo-Academy fighting style, a martial art often employed during discussions and negotiation.
Toradora!: Ryuuji, due to having the Face of a Thug, tends to deliver these by accident. At one point he looked at Taiga while trying on some makeup for the role of a villain in a play and frightened her so badly she hit him. It gets even worse on the few occasions that Ryuuji is actually trying to look scary. The school festival race stands out in this regard.
Anak from Tower of God who sports this when she realizes she is facing her parent's murderer. Who just took her keepsake out of his mouth.
Tsubasa -RESERVoir CHRoNiCLE- has Kurogane and his death glare which he normally directs against Fai and Mokona. Where enemies shake with fear, well... while Mokona at least seems slightly affected, Fai shows absolutely no indication of fear or uneasiness at all. Could be because Kurogane would never really hurt him but he would definitely hurt Mokona.
In Umineko: When They Cry, you certainly don't want to be a servant of someone who can deliver a glare like this,at the end of the page.
Vampire Knight: A lot of characters will give you this, though Kaname and Zero especially.
In Yona of the Dawn, the titular princess has been known to terrify grown men with a single look. When the corrupt Lord Kumiji begins to abuse his prisoners and followers alike, Yona's furious glare causes him to drop her and recoil in shock. He's surprised to find his hands trembling, with just a look from a girl.
Seto Kaiba of Yu-Gi-Oh!, according to Fanon, uses this all the time. In reality, he doesn't use it so much... unless your name is something other than Mokuba. Unsurprisingly, Kaiser Ryo has a very potent one of his own.
Hiei from YuYu Hakusho is really good at these along with being a hostile Perpetual Frowner. However, what is truly impressive is that when really pissed off, both Yusuke and Kurama managed to pull Death Glares that make any of Hiei's look like fluff in comparison.
Put simply, if Hiei or Yusuke give you a Death Glare, prepare for a severe ass-kicking. If Kurama gives you a Death Glare, run.
Zatch Bell!: Gash uses a Death Glare that inexplicably stops the far stronger Vincent Bari in his tracks. Other characters have also exhibited death glares, but not to the extent that Gash does.