In an unnamed suburb somewhere in the midwest, one lone woman spends her nights patrolling her nine-block neighborhood, protecting her neighbors from crime. Usually, it's a quiet patrol, but lately, people just outside her patrol have disappeared, including her ex-boyfriend John, and she has sensed someone watching her. Could it just be coincidences, or is it her mysterious arch-nemesis, the Dusk Devil?
This series contains examples of:
- Ambiguous Disorder: Twilight Guardian has an unnamed mental illness for which she has been prescribed medicine, which she refuses to take because it supposedly dulls her senses. According to Troy Hickman, she was originally written as having OCD, but her behavior in the series suggests some form of paranoid personality disorder. Issue #4 reveals that her father is also a paranoiac.
- Darker and Edgier: One of the pitches that Twilight Guardian receives re-imagines her as a violent, gun-toting vigilante.
- Disappeared Dad: Twilight Guardian's father disappeared when she was very young, and her mother suspects that he might have been a serial killer, given that his disappearance was preceded by a string of other disappearances in the area. In Issue #4, he appears and explains that he walked out on Pam and her mother because he'd become convinced that the government was planning to frame him for the disappearances.
- No Celebrities Were Harmed:
- Punny Name: This series abounds with them, particularly in TG's massive comic-book collection. Highlights include Bananas Froster, a super-intelligent monkey with a freeze ray; Oktoberfist, a German bruiser; and Slaughterdammerung, a Wagner-themed Thanos expy. TG herself gets in on the act, adopting the pseudonym "Eve Ward" when she travels to Madeline Island.
- Shout-Out: The comic-book convention in Issue #2 feature posters and cosplayers for various other Top Cow properties, like Witchblade and Common Grounds.
- Stripperific: In issue #3, TG receives several proposals for a comic book based on her. One of the proposals, by a British writer named Ian St. Switherington, imagines her as a foul-mouthed nihilist who runs around nearly topless except for a pair of suspenders.