The leader of the Toad Patrol and also the oldest. He has a strong sense of leadership and does everything in his power to get all of his siblings safely to Toad Hollow. He sometimes doubts in his abilities, but family and friends are always near to help him realize how important his role is within the group.
- Alliterative Name
- Character Development: Gains the confidence to lead the Toad Patrol by the end of season 1.
- Rousing Speech: Tends to be the one to fire these off, most notably in "The Cure", "A Cry For Help", and "The Lost Symphony".
- Supporting Leader: Though he has some development in learning to truly embrace his role as a leader, his focus is notably shorter than some of the other characters.
The second oldest and the unofficial second-in-command. She puts everyone else first and tends to come off as naggy to the younger ones who she only seeks to protect.
- Character Development: Learns to not be so overprotective to her younger siblings, and in season 2 she grows the ambition to become a healer.
- The Chick
- Cool Big Sis
- The Medic: In season 2.
- Peek-a-Bangs: Appears this way in the first episode, before tying it back in a braid. Strangely enough, she also has this hairstyle in one of the promotional images.
- You Shall Not Pass!: Attempts this in "Invasion", planning to attack a pair of weasels long enough to give Oyster and Panther Cap a chance to escape, despite the fact that she wouldn't stand a chance. Luckily, all three toadlets fall into a trap door before she can go through with it.
She's the third oldest but could hardly be considered the third in command. She is wild, loud and stubborn. And surprisingly, she shares a strikingly similar personality to Fur Foot.
- Better to Die than Be Killed: Suggests that the group jumps into freezing water in "Castaway" as Medea slowly closes in on them.
- Bratty Half-Pint: She's the only member of the group to blantantly disobey Fur Foot and Beauty Stem on purpose. Luckily, she grows out of this as the series goes on, and "Winter Woes" beats what little of this trope remains right out of her.
- Character Development: Was a complete brat during certain episodes in season 1, then luckily grew out of it by early season 2.
- Dreadful Musician: Though she is considerably better by season 2.
- Hypocrite: Somehow forgets the lesson of "Trojan Duck" by the next episode. Not helped by her own distaste towards hypocrites as well.
The fourth oldest of the crew. Occasionally others have wondered if his head is screwed on correctly. He comes off as being airy, naive and having his head in the clouds but surprises the others when he shares words of wisdom.
- Cloudcuckoolander: He's definitely the most airheaded of the group.
- The Exile: Attempts to do this to himself in "The Giver of Names". Fortunately, Cleopatra convinces him to return.
- The Klutz: If anyone has to fall into this, it's usually him.
- The Nicknamer: Spends most of the show making up names for the things the toad patrol encounter, anything from cars (Rumble-crushers), to the sky (big blue).
The fifth oldest and third in command (even though he is not fit to be leader due to hating the concept of decision-making). He will easily come off as being pessimistic, sarcastic and easily irritable but it's a rare occasion that Puff Ball will even so much as raise his voice.
- The Big Guy
- Big Little Brother: Despite being the fifth oldest, he seems to be ever so slightly taller than everyone else.
- Deadpan Snarker: Thrives on this.
- Jerk with a Heart of Gold
- Stout Strength
Oyster is the second youngest member of the Toad Patrol and the twin sister of Slippery Jack. Despite her age, she will never hesitate to get involved or help others.
- Bare Your Midriff
- Cheerful Child: Besides a slight bout of depression in "Choices", she is one of the most optimistic of the team.
- Polar Opposite Twins: With Slippery Jack.
Slippery Jack is the second youngest member of the Toad Patrol and the twin brother of Oyster. Thanks to his great intellect and drawing skills, he is able to steer his brothers and sisters in the right direction when travelling through the Great Forest.
- Child Prodigy
- Claustrophobia: Though considering that by the time it becomes evident is after several occasions where being trapped nearly got him killed, can you blame him?
- Didn't Think This Through: Gets the wonderful idea to thaw out Mistle Toad, who's frozen in ice, by placing him over a steam vent. What he fails to account for is how this will lead to Mistle Toad falling into the steam vent once the ice melts.
- Ditzy Genius: Is intelligent enough to form a plan to slide an icicle to a very specific place using purely physics, but forgets that a tunnel has two exit points.
- The Navigator: Receives the tools to make maps in episode 4 and uses them for the rest of the series.
- Nerd Glasses: Wears these.
- Out of Focus: Of the main characters, he tends to have fewer arcs than the others. Only two episodes (Scooped and The Lost Symphony) had him in any major role, and even then he was paired with another character throughout (Oyster and Shaggy Mane, respectively).
- The Short Guy with Glasses
- The Smart Guy: Pretty much the entire basis of his character.
Panther Cap is the youngest of the eight toadlets that form the Toad Patrol. Ironically, he's the most important member of the group since he's the only one who can guide his brothers and sisters to the Fairy Ring.
Mistle Toad is an adult toad who lives in the Great Forest. Other than his obsession with the Ancients, not much is actually known about him.
- Harmless Freezing: The toad patrol find him frozen in an icicle in "The Castle of the Ancients", and are easily able to free him without any ill effects.
- Odd Name Out: Partially due to being the first character created for the show, being among the only ones to have a name that's not either a type of mushroom or named after a historical or famous fictional character.
- Reasonable Authority Figure: Despite some... questionable actions, he has been a reliable resource to the toad patrol when they truly need him.
- Took a Level in Badass: Outright tackles Erebus in "The Fairy Ring"
Earth Star is a minstrel toadlet born a season before the Toad Patrol. He wanders around the Great Forest just like Mistle Toad, going wherever his feet take him. Because of this, he has many connections spread throughout the forest and never hesitates to call on their help when needed. Earth Star is also the only toadlet to have ever returned to normal after being turned into a Toadstool, although the reasons for this are still unknown.
- Cool Shades: Sports a pair of these.
- Baleful Polymorph: Turns into a toadstool in "The Sacrifice".
- Dark and Troubled Past: Reveals in episode 2 how his last group didn't fare well, and that he's the only one left.
- Dogged Nice Guy: Spends the first season trying to gain Beauty Stem's affection. Luckily, she seems to be warming up to him by the start of season 2.
- It's All My Fault: Blames himself for indirectly causing his last group and him to all turn into toadstools.
- Love at First Sight: Is utterly infatuated with Beauty Stem after she says one sentence.
- Put on a Bus: He stays behind in Toad Hollow when the others go to rescue Mistle Toad in season 2, and doesn't even get as much as a mention again until "The Castle of the Ancients". The Bus Came Back in "Choices".
- Second Episode Introduction
- Survivor Guilt: Despite how he acts in front of the toad patrol at first, it becomes more obvious as the series goes on how he hasn't really gotten over what had happened to his last group. Made worse knowing that it actually is his fault they all turned into toadstools.
- The Woobie: Between his tragic backstory, deep set anxiety, and the events of "The Sacrifice", it's hard not to want to give him a hug.
- Voiced by Kate Hurman
A mysterious healer toad who lives in seclusion.
- Cool Old Lady
- Everyone Calls Her Barkeep: She's never reffered to by an actual name.
- Mysterious Past: Was apparently among the ancients, is one of the oldest characters in the show, hates Mistle Toad enough to outright have banned him from her house... We get explanation to exactly none of this.
A mute salamander residing in the Castle of the Ancients.
- Cute Mute
- Mr. Exposition: Despite being mute, he plays this role by revealing that Earth Star is turning into a toadstool because he was the one to first attempt to play the ancient symphony.
- Not What It Looks Like: Puffball immediately assumes that his presence in the maze means he's after the toadstools. Sigmund, being mute, can't dispute this. Thankfully it's cleared up within a few minutes.
- Simple Staff: Has one that doubles as a shovel.
- The Speechless
- Voiced by Norman Mikeal Berketa
A bombastic frog the toad patrol meets on their journey.
A mole that becomes an honorary member of the toad patrol.
- Voiced by Rick Jones
An evil snake
- Gratuitous English: Says "Hi there!" once in the Croatian dub.