A young guardian assigned to the Waterford house in the second season.
- Alas, Poor Villain: Despite him crudely treating women previously, his death is seen in a more sympathetic light, especially for both Serena and June.
- Defiant to the End: He remains wordless and unrepentant during his execution.
- Drunk with Power: When the Waterfords go to Canada, he is left in charge and it goes to his head. Other than Eden, he treats the women at the household with disdain, orders June and Janine to stop talking, and clocks Janine when she acts out.
- Improbable Age: He's around 20. Rita comments how he is so young but he's still in charge over her and everyone else in the house, when the Waterfords are away.Rita: That boy downstairs is maybe 20 years old. And he can tell me to do anything. He could break my jaw, and no one would say boo.
- Star-Crossed Lovers: Falls in love with Eden who reciprocates, but she is married to an older guy. Given the Crapsack World they live in, it ends badly for them.
- Through His Stomach: His flirtation with Eden involve him enjoying the food she cooks.
- Would Hit a Girl: He hits Janine with his gun when she talks back to him.
A Contact who Nick used to have sexual relations with. She was a Martha who works at the kitchens in Jezebel's, but she's later reassigned to Commander Lawrence.
- Childfree Is Not Allowed: She is considered a "sinner" because she had her tubes tied.
- Deadpan Snarker: She has a sarcastic and friendly disposition towards Nick. She continues her snarking tendencies with June.
- Friends with Benefits: Though Nick forgoes sex with her during his affair with June.
- Shipper on Deck: She's never met June in Season 1, so she good-naturedly teases Nick about his affair. It's unknown if she knows June is the same woman Nick had talked about previously.
- The Informant: She provides information to Nick in exchange for illicit products, such as alcohol, prescription drugs and pregnancy tests. It's assumed that she is still in this role in Commander Lawrence's household.
A Martha assigned to Commander Lawrence's household.
- Ambiguous Situation: Her fate after Lawrence announces he "sent her away" is unrevealed. Its unknown if he actually had her reassigned or executed.
- Haughty Help: When the Lawrence household is introduced, she has a rather equal relationship with Commander Lawrence, more of old friends than that of master and servant.
- Red Right Hand: She is missing an eye.
- What Happened to the Mouse?: See Ambiguous Situation above.
A young Martha assigned to Commander Lawrence's household after Cora is "sent away".
- The Baby of the Bunch: How June and Beth regard her at first, not just because of her apparent age, but mostly due to her fearful and submissive demeanor.
- Bring My Brown Pants: She has a tendency to wet her pants under extreme stress.
- Nervous Wreck: At first she's terrified of Lawrence and even pees herself.
- Replacement Goldfish: For Cora.
- Took a Level in Badass: She grows more assertive as episodes go by, insisting she wants to aid June and that she's capable of doing it. Later, she takes part in the smuggling of the escaping children along with the rest of the Marthas and Handmaids.
A young Martha with whom Emily carries on a secret affair.
- Bury Your Lesbians: She's unceremoniously executed due to the laws of Gilead against homosexuality.
- Star-Crossed Lovers: With Emily.
- You Are Number 6: Her real name is never mentioned onscreen.
- You Have Failed Me: Since she's infertile, she's judged as an Unwoman, unworthy to be kept even as a servant, and hanged. Emily is spared only because she's fertile.
- It's implied that she may have a long laundry list of related "crimes" in order to warrant execution. Emily would later be sent to the Colonies despite her committing a murder, so something else this Martha supposedly "did" seriously pissed Gilead off for her to be offed in this manner.
A Martha in the McKenzie household.
A Martha assigned to Jezebel's.
- Chekhov's Gunman: She's revealed to be one of the five women captured in Chicago that were chosen by June to be spared from the Colonies.
- Disposing of a Body: Maureen and the other Marthas dispose of the body of High Commander Winslow after June kills him in self-defense, leaving no trail of his presence.
A Martha from Lexington that joins the plot to have a group of children smuggled into Canada.
- Screw This, I'm Outta Here!: Maggie changes her mind and tries to return to her household along with Rebecca/Kiki, but the ends up leaving the child to flee alone.
- What Happened to the Mouse?: Janine reports Maggie was arrested. Its unknown what happens to her afterwards but its likely she'll be executed.
June and Luke's daughter, born before the rise of Gilead. At the start of the series, she was taken from her parents while trying to flee Gilead and placed with the Mackenzie family where she is given the name Agnes.
- Abusive Parents: The Commander and Wife she was assigned to have physically punished her, "only twice".
- Defrosting Ice Queen: Due to the trauma of separation, she can barely bring herself to acknowledge June when they're briefly and secretly reunited, having accepted her new parents and resented her mother for not 'trying hard enough' to find her, but she eventually breaks down crying and calls her Mommy.
- Don't Make Me Take My Belt Off: The Martha from their household says to June that Hannah/Agnes was physically punished by her new parents "only twice". With all their obsession with Biblical allusions, "spare the rod" is very likely presented as the correct parental discipline and beneficial to children.
- Feminine Women Can Cook: Mrs. McKenzie says to June that she (Hannah/Agnes) is actually quite a good cook. One of the few things girls in Gilead are allowed to learn and a properly feminine hobby for daughters. June is delighted to hear it because Luke cooks.
- Meaningful Rename: After her forced adoption, her new parents have renamed her Agnes (meaning "purity"), presumably to erase her connection to her 'sinful' parents.
- Named by the Adaptation: She was never referred to by name in the novel, presumably because Offred wanted to protect her identity.
- Put on a Bus: Due to Ofmatthew's treachery, the entire Mackenzie family is relocated and no one, including June or Commander Lawrence knows where they are.
- Race Lift: In the novel, she is described as being blonde, whereas in the series she is biracial.
- Textile Work Is Feminine: Her "adoptive mother" says that she likes to sew, but that she's not very good at it. Sewing and other textile work is one of the few things daughters are allowed to learn and pursue as it's perceived as a properly feminine interest.
June and Nick's daughter, conceived and born in Gilead. She now resides with Luke and Moira after Emily was able to smuggle her across the border.
- Dead Guy Junior: When she is born, June names her Holly, after her mother.
- Improbable Infant Survival: Against many odds, she survives being submerged in cold water while Emily crosses a river to reach Canada.
- Meaningful Rename: June names her Holly upon her birth, but when Serena claims her for herself, she is given the name Nichole. As a nod to Serena allowing Holly to escape, June tells Emily (who is taking her to Canada) to call her Nichole as well. Even with Luke knowing her real name and parentage later on, both Luke and Moira continue to call the baby Nichole.
- Protectorate: Both Moira and Luke act as Mama Bear and Papa Wolf to Holly/Nichole while she is in Canada
- Small Role, Big Impact: Variation. She doesn't do anything, given that she is a baby. But her very existence is what drives the plot in Season 3 where High Commander Winslow wants to use her as a tool to force Canada to deport all Gilead refugees, while Serena goes behind the scenes to try to get back together with Nichole.
A 15-year-old Econowife (forcibly) assigned to Nick.
- Arranged Marriage: Gilead pawns her off as Nick's new wife.
- Children Are Innocent: Poor Eden doesn't seem to realize how awful Gilead is and is molded to believe it's 100% dutiful.
- Defiant to the End: Despite being hugely brainwashed to obey Gilead, she grows disillusioned enough with her marriage to try to defy Gilead when she falls in love with a more age-appropriate boy. At her execution, she does not renounce her sin but simply recites a Biblical verse.
- Hidden Depths: June finds a heavily annotated Bible among her personal belongings, suggesting that Eden did not buy into Gilead's doctrine to the extent that she appeared to - specifically, she was reading (which Gilead does not permit for women) and trying to understand or analyse the Bible beyond what Gilead teaches.
- I Let Gwen Stacy Die: Eden's execution weighs pretty heavily on Serena Joy, seemingly convincing her that Gilead is not safe even for the most obedient and True-Believing of girls. This helps facilitate her HeelFace Turn and specifically, her decision to let June escape with baby Nichole.
- Mal Mariée: Mal Mariée means "badly married" and it refers to a young girl unhappily married to an old guy. 15-year-old Eden is forcibly assigned to marry Nick Blaine. Nick is in love with June who are both in their thirties. Eden tries to make the marriage work, but Nick's not interested in her, which she takes hard. She soon falls in love with guardian Isaac who is about her age. In a twist, she's exasperated that Nick is not jealous at all and angry that he doesn't even care she kissed another man. After chatting with June, who advises her to grab whatever chance at happiness she has in their crapsack world, Eden and Isaac run away. They are caught and executed Eden for infidelity, Isaac for desertion.
- Morality Pet:
- Despite his cruel nature, Isaac comes to love Eden and tries to elope with her. But they both get caught and tragically die.
- Serena Joy, as awful as she may be, tries to look out for Eden, and Eden's execution weighs heavily on her.
- Kill the Cutie: She is executed for infidelity after running away with Isaac.
- Unwanted Spouse: To Nick, he doesn't want their Forced Marriage and is not interested in her. Eden tries to be nice to him and make it work for some time.
- Woman Scorned: She comes to resent Nick's avoidance of her and neglect of their marriage, and picks up on his attraction to June.
- Your Cheating Heart: Nick catches her kissing Isaac. She immediately runs to him begging forgiveness, but becomes upset with him when he tells her that he doesn't care.
June's feminist mother. She was a doctor (possibly an Obstetrician) before the Gilead takeover.
- A Fate Worse Than Death: When Aunt Lydia is showing the handmaids photos in the Red Centre, June and Moira see one of her in the Colonies.
- Cool Old Lady: Blasts 'Hollaback Girl' by Gwen Stefani whilst riding in June's car with her feet on the dash.
- It's a Small World After All: Dr. Yates, an Obstetrician in Gilead, claims he knows Dr. Maddox but it may be purely based on reputation. This does make the implication that Holly is an obstetrician herself based on this.
- Parental Marriage Veto: Downplayed, but she seriously disapproved of June marrying Luke.
- Soapbox Sadie: While her feminist views are portrayed rather sympatethically (and as the polar opposite of Gilead's ideology), she can have a bit... strong opinions. Marriage? Wasting one's energy for "just a guy" (said when her daughter announces her engagement to her). Baptism? "Having your child sanctified by a bunch of Holier Than Thou child molesters" (said in a church). Ah, and she insist that the whole family dines in a vegan restaurant despite, by June's words, "not even being a fucking vegan".
- Why Couldn't You Be Different?: Again downplayed, but she doesn't understand June's happiness with her editing job, marriage, and normal family life and compares her unfavourably to Moira, who is more involved in political action and resistance.
An intelligence agent for the Loyalist United States forces, operating out of Canada.
- Black-Tie Infiltration: Tuello's introduction comes during Season 2, when he chats with Serena at the Canadian hotel bar, while Fred is negotiating international treaties with the Canadian Government.
- Crazy-Prepared: Before his initial meeting with Serena, he had done extensive research on her and knows exactly what it would take to get Serena to turn on Gilead.
- Chekhov's Gunman: While he's first introduced trying to woo Serena into defecting to the Loyalist side, he eventually delivers the instrument of Fred's downfall to Serena: the cell phone she uses to arrange his arrest.
- Everyone Has Standards: He can barely conceal his disgust when Serena shows her true colours by referring to June as Offred.
- Kick the Son of a Bitch: He gets to do this to both the Waterfords; firstly by arresting Fred for crimes against humanity, and then later informing Serena she's going to face the same charges, since she doesn't have immunity from being prosecuted for coercing Nick to conceive a child with June that she could claim.
- Shut Up, Hannibal!: He gives one to Serena, when after she insists she'd never commit treason, Tuello retorts she already did when she assisted in the rise of Gilead.Serena: [referring to Gilead] If you had done better research, you would know that I would never betray my country.Mark: [referring to America] I thought you already did.
- He gets to do it to her again in the Season 3 finale: when Serena tries to defend her actions by claiming Nick and June were in a consenting relationship, Tuello retorts that she still coerced them into doing so by threatening June's safety, therefore Serena's actions still constitute rape.
- Wham Line: Delivers one to Fred with a gun in his face in the episode Liars."Commander Waterford, you have now crossed into Canada. We are arresting you for war crimes and crimes of aggression in violation of international human rights treaties and international law."
Mrs. Waterford, under warrant from the ICC and the government of the United States of America, I am placing you under arrest.
- He then delivers another to Serena, after Fred provides Tuello with some damning information on her.