Voiced by: Enric Cusí (1994 series), Karra Elejalde (2014 movie)
Played by: Benito Pocino (2003 movie), Edu Soto (2008 sequel)
- Almighty Janitor: In a sense. His disguise skills border Reality Warper field, and he has occasionally shown to be a very efficient agent when getting serious, but he is still a low ranked TIA agent with a painfully low payment. Justified because the rest of the time he is a bumbling moron.
- Ambiguous Disorder : Absolutely clueless in social situations , especially with the upper class ? Check. Has a unfortunate tendency to talk and take things literally ? Check. Misintrepret constantly words and orders ? Check. Obsessed by disguises to the point it might impact others negatively ? Check.
- Bald of Awesome: Subverted. He does not have a single hair on his head, and while he can perform some awesome feats, that doesn't make him an awesome secret agent. Interestingly, he had a spectacular mane of hair back when he joined the TIA, but lost all of it due to an experimental lotion by Dr. Bacterio (and never forgave him for it).
- Characterization Marches On: In the series's beginnings, he carried an umbrella and wore a bowler hat which served as a Hammerspace, and also had perpetually squinted eyes and his mouth always hidden by his shirt's neck. He dropped all of those traits pretty fast.
- Creepy Mortician: People often mistake him for one because his attire is similar to a classic Spanish undertaker uniform. In fact, whenever he really disguises himself as one, his clothing barely changes.
- Gag Nose: His is bigger than average even in the series's drawing style.
- Genius Ditz: While certainly not the sharpest knife in the drawer, he can be an actually competent agent when getting serious, and he's far more likely than Filemón to come up with ideas to save the day.
- Hillbilly Horrors: He was born in a deep country village, apparently in Valladolid, Castilla y León, which is portrayed this way.
- Impossible Thief: Although this trope is used very often in the series, Mortadelo is the one who does it the most frequently, switching a valuable item, a MacGuffin or himself for something completely useless like a master. However, this also has an unfortunate tendency to backfire when he accidentally does the switch in reverse or fails to do it altogether.
- Kibbles and Bits: A human example: his disguises usually feature his white shirt collar, even whenever their forms are not humanoid or technically wearing anything. In fact, barring a few times that would qualify as Early Installment Weirdness, Mortadelo himself keeps the collar on even when barechested or entirely nude.
- Latex Perfection: Sometimes uses latex masks in his disguises.
- Let's Get Dangerous!: When chance calls for it, he can be surprisingly cunning, not to talk about his shapeshifting abilities.
- Limited Wardrobe: Always wears a black suit with matching shoes and frock coat. Some chapters have revealed his entire wardrobe is full of identical suits.
- Manchild: While more or less all the main cast qualifies for this, he is possibly the biggest example, being also a bit of a Cloudcuckoolander.
- Master Actor: Subverted. While he does sometimes accomplish perfectly the tasks of his disguises, he usually only has the basic knowledge on how to do keep up the deception and without fail provoke major disasters
- Master of Disguise: Zig-zagged. He is an expert on disguises, but how efficient they are is heavily mandated by the needs of the plot. He sometimes uses Wig, Dress, Accent or very amateur-looking party suits, which get his cover blown up quickly, but most of the time he dons disguises of animals, objects, vehicles and other things that amount to literally turning into them, making him indistinguishable. The only flaw is that he is usually keeping his face and or white collar, but this never alerts the antagonists.
- Master of Unlocking: Another of his most useful skills, to help when trying to open a door or a safe, is lockpicking. However, for the sake of jokes, his favorite method is using a "Master Key" that is simply a giant key to use as a makeshift battering ram.
- No Sense of Direction: Whenever he's tasked with driving a car, a boat or flying a plane, chances are he'll arrive at the wrong location, if he even manages to get anywhere without crashing the vehicle.
- Shapeshifting Failure: Subverted. His disguises usually show the shape of his face and his shirt collar, but this is rarely noticed, which suggests it is more of an artistic resource for the reader than an in-universe feature.
- Uncanny Family Resemblance: His grandmother is identical to him, only with a larger chin.
- Undying Loyalty: To Filemón, whom he calls his "boss". Not matter how much Filemón berates or mistreats Mortadelo, the latter never resents him.
- Unskilled, but Strong: He rarely makes a good use of it due to his dim-witted nature, but having the skill to turn into anything without limits is an asset that few antagonists can match.
- Vague Age: The comic's original script said he and Filemón were technically middle-aged, but they were later portrayed as men in their 30s and it sticked until today.
- Voluntary Shapeshifting: His disguise ability equals to it. He can turn into pretty much anything, including massive objects and abstract concepts.
Voiced by: Xavier Martín (1994 series), Janfri Topera (2014 movie), Ramón Langa (2014 movie, imaginary self)
Played by: Pepe Viyuela
- Almighty Janitor: Another justified example. Promotional materials state he could be an unambiguously excellent agent if he dedicated as much brainpower to his job as he dedicates to chasing Mortadelo for his failures.
- Bald of Awesome: Except by exactly two hairs, he is bald. Another subversion, as he is not as awesome as he thinks.
- Born Unlucky: Has possibly the worst luck of all the cast, and unlike them, he does not have shapeshifting powers or a high rank to compensate it.
- Butt-Monkey: He is constantly suffering all sorts of accidents and mishaps, often but not always caused by himself (and more than often caused by Mortadelo).
- Characterization Marches On: He was hit the hardest at it. In the comic's beginnings, he smoke on a pipe, wore a hat and a jacket, and his nose was slightly larger and curvier. He was also a Perpetual Frowner, and his surname was Gutiérrez, not Pi. He dropped most of those traits, although he retained the jacket for a comparatively longer time.
- Distinguished Gentleman's Pipe: Only initially. During The '90s it was occasionally shown to smoke regular cigars, and he still has the penchant to steal the Supervisor's Cuban ones.
- Hair-Trigger Temper: Usually quite justified.
- Hyperspace Arsenal: Whenever he chases Mortadelo, he wields giant weapons of several kinds. A tie-in book claims that he would be a better agent than James Bond if he bothered using them against actual enemies.
- Hypocrite: Claims to be an ideal worker, but he steals the Supervisor's expensive wine and cigars and talks behind the people's backs (and is punished for it).
- Jerkass with a Heart of Gold: He is rigid, very hot-tempered and also a bit pedantic, but not a bad guy overall, and he cares deeply for Mortadelo even if he would die before admitting it.
- Limited Wardrobe: White shirt, black bow tie, red pants (very rarely blue) and brown shoes. He also wore a red jacket in his beginnings.
- Only One Name: Inverted; he is the only with an official, established surname, even if it is rarely mentioned. The rest of the cast either lack it or have it only informed in promotional materials.
- Sherlock Homage: Started as this, as said in Characterization Marches On above. He even got once to wear a hunting coat and hat as Holmes is often portrayed.
- Tsundere: A type B male example towards Mortadelo.
- Vague Age: Like Mortadelo, he was supposed to be middle-aged, but later chapters went for a 30-something portrayal.
Supervisor Vicente Ruínez ; El Súper
Voiced by: Miguel Ángel Jenner (1994 series), Mariano Venancio (2014 movie)
Played by: Mariano Venancio
- Badass Mustache: Has an impressive mustache, although it is actually the only badass thing on him.
- Bad Boss: He is despotic, intransigent and quite short-tempered, and it is sometimes hinted he is embezzling the TIA's money for his personal luxuries. Also, he has no qualms about sending Mortadelo and Filemón to suicide and/or impossible missions without even hiding it. Also, he never bothers to hide the fact that he thinks very low of them.
- Bald of Awesome: Another subversion. He lacks hair on top, but he is not exactly a great example for the rest of the TIA.
- Bastard Bastard: A promotional material revealed he is the son of his family's butane gas man.
- Da Chief: As Mortadelo and Filemón's immediate superior, he is the one giving them their missions.
- Deadpan Snarker: Especially towards the titular agents. Most of the time, it is used to show his skepticism towards their promised success.
- Embarrassing Last Name: His pseudo-canonical surname, Ruínez, can be translated to English as "Ruinson".
- Fat Bastard: He is wide-waisted and easy to get angry.
- Hair-Trigger Temper: Like Filemón, with good reasons.
- Hypocrite: He enjoys a hefty paycheck while the rest of the TIA is heavily under-budgeted, and brushes it off when it is pointed.
- It's All About Me : A good number of the earlier stories where he sends M &F searching for a MacGuffin , it turns out to be for a very petty reason (pack of cigarettes, embarrassing secret...). The best part is he does seem confused and shocked by his agents (justified) anger.
- Jerk with a Heart of Gold: Even although he has a nasty personality and treats his subordinates like dirt, it has been shown that deep down he really cares for them and sees Mortadelo and Filemón as a sort of mischievous sons.
- Large and in Charge: In weight more than in height, although this depends on the scene; he is sometimes shorter than Filemón and sometimes of the same height. However, in Dibújalos tú solito, an instruction manual on how to draw the characters, Ibáñez says himself that El Súper is in fact taller than Filemon.
- Limited Wardrobe: A blue suit with a black neck tie.
- Mr. Exposition: He briefs the title duo about their missions, only bringing in Dr. Bacterio whenever it is a science-related affair.
- Only One Name: In the comics themselves, he is just known as Vicente. His surname was officially revealed later (and with some controversy, as a source claims his surname is Patente while other presumably higher says it is Ruinez).
- Pet the Dog: Believe it or not, in the earliest stories, he was genuinely rewarding Mortadelo and Filemon when they succeed (albeit quite painfully) their mission. Unfortunately, said reward was linked to a series of Amusing Injuries received by Mortadelo, Filemon or both during said mission (Ex: whisky with ices cubes after going to the North Pole) and they retaliate by beating the crap out the Super. Take La caja de los diez cerrojos as an example of this Running Gag.
- Pointy-Haired Boss: Downplayed. He is not an example of competence, but he compares favorably to the title agents, and it is implied he is overall a good executive when he does not have to directly treat subordinates.
- Running Gag: It is common for Mortadelo and Filemón to misunderstand comically his briefing of the mission, if not ignore it completely because they have their attention put in another thing, or even snark at him if the mission is particularly dangerous or unpleasant. In any of those cases, he reacts violently against them, generally hitting or strangling them.
- Team Dad: Age wise, he frequently acts as a father figure to Mortadelo and Filemón, sometimes to his chagrin.
- The Scrooge : He has a lot of flaws but he is exceptionally stingy and barely pays his subordinates. Mortadelo and Filemon will always have to use an antiquated vehicle or weapon and it will be completely useless on their mission : even worse, it is heavily implied that the Super is embezzling the TIA money for himself.
Voiced by: Emilio Freixas (1994 series), Enrique Villén (2014 movie)
Played by: Janfri Topera
- Absent-Minded Professor: Mostly averted, although he can fall on it from time to time, especially when the plot calls for one of his inventions to be stolen or lost.
- Almighty Janitor: Bacterio is undoubtedly an extraordinary inventor, as while his gadgets and serums often turn out either Gone Horribly Wrong and Gone Horribly Right, they still have amazing effects that could be harnessed with adequate testing and used in mankind's benefit. However, he is confined to work in a shady secret service instead of gaining millions with his patents, and is almost bizarrely okay with it.
- Badass Beard: Has a big, bushy black beard that covers his mouth except when widely grinning. It gets invariable burned off or grabbed.
- Bald of Awesome: Another subversion, although it could be said that many of his inventions are unambiguously awesome.
- Butt-Monkey: He is constantly beaten up by Mortadelo and Filemon and sometimes the Super and Ofelia when become victims of his last inventions, something which happens all the time
- Deadpan Snarker: Has his moments, which can turn into Snark-to-Snark Combat with Mortadelo due to their personal enmity.
- For Science!: Many of the missions given to the titular duo are testing Bacterio's creations.
- Gadgeteer Genius: Is able to create machines that do almost everything, although whether they work as expected or not is another thing.
- Limited Wardrobe: A green suit, sometimes with a black neck tie.
- Mad Scientist: Mortadelo considers him one, and it is not inaccurate given that Bacterio checks many of the points (he builds things without blueprints, uses obviously forced experiment subjects, does self-experimentation, is a bit of a sadist, etc). However, he works for the good guys and has never shown to be anything other than a dutiful government agent.
- Meaningful Name: Bacterio, as in "bacterium".
- Morally Ambiguous Doctorate: He is addressed as both "doctor" and "professor", but he tends to be officially billed as the former.
- Mr. Exposition: Although most of the time it devolves into Techno Babble that get his audience slept or dizzy.
- Nice Guy : Downplayed. He is the most even-tempered of the main cast as he is spending most of his time minding his own business and is usually being very polite and approchable when not inventing. Still, as mentionned in the Mad Scientist entry, Bacterio has no problem using his own colleague (especially Mortadelo and Filemon) or himself as Guinea Pigs.
- Non-Action Guy: He is never seen wielding weapons or engaging in fighting, as he is not a field agent.
- Omnidisciplinary Scientist: Subverted. Initially, he was simply billed as a biologist and only produced serums and drugs, but over time he became a generic scientist who navigated all the fields of expertise needed by the plot.
- The Professor: A half parody of the trope.
- Professor Guinea Pig: Got turned into a midget in his very introductory chapter after testing a new growing serum on himself.
- Shorter Means Smarter: Dibújalos tú solito, a promotional album for drawing the characters, reveal that he is the shortest of the main cast. This is pretty much an Informed Attribute, as most scenes portray him as roughly similar to Filemón and El Súper in height.
- The Smart Guy: He is in task of all intellectual and/or scientific matters.
- Teeth-Clenched Teamwork: He and Mortadelo hate each other, as Bacterio made him lose all his hair and Mortadelo often tries to get revenge for it, but are forced to work together.
Voiced by: Rosa Pastó (1994 series), Berta Ojea (2014 movie)
Played by: Berta Ojea
- Abhorrent Admirer: She is attracted to Mortadelo and sometimes to Filemón, but they are both repulsed by her ugliness and fatness.
- Berserk Button: Being called fat either directly or indirectly. Damaging her clothing or makeup is another safe way to get her angry.
- Big Beautiful Woman: Although she is round plump, there are characters who find her attractive, especially those who are overweight and/or obese like her. She has used this to play a Heroic Seductress role as a last resource.
- Dreadful Musician: She cannot absolutely sing.
- Kev Lard: Once deflected a Death Ray back to its origin through her breasts.
- Lethal Chef: Her food often turns out toxic and/or disgusting.
- Limited Wardrobe: A red dress with black kneeboots, although she can don a great variety of attires if the story needs it.
- Sassy Secretary: Though mostly towards Mortadelo.
- Sexy Secretary: She believes herself to be it (although recent chapters have hinted she is at least somewhat aware of her excess of weight), while the reality is quite the opposite.
- Stout Strength: Despite or because her obesity, Ofelia is a physical powerhouse to Super Strength levels.
Voiced by: Alicia Laorden (1994 series), Athenea Mata (2014 movie)
- Adapted Out: From the live action adaptation.
- Blondes Are Evil: Downplayed. She was not evil, but could get quite mean.
- Chuck Cunningham Syndrome: Ibáñez made her disappear three years after her debut in order not to overshadow the rest of the cast.
- Foil: He was a blonde secretary, just like Ofelia, but unlike her, Irma was thoroughly sexy and had Mortadelo and Filemón chasing her. They had a bitter enmity as a result.
- Hello, Nurse!: Mortadelo and Filemon were rendered catatonic every time she entered the room.
- Ms. Fanservice: To the point she was officially introduced in the series to show Mortadelo and Filemón weren't homosexuals.
- Sexy Secretary: A straight example, unlike Ofelia. Actually, in her first appearance, Irma was the chief of the TIA's terrorism section - she was only degraded to secretary after a failure caused by the titular duo.
- Small Role, Big Impact: She was in the series for just three years, but that amount of time was enough for her to become one of the most memorable secondary characters in Mortadelo y Filemón, to the point people who don't follow closely the series are often unaware that she is not a part of the cast anymore. She was also brought back for the 2014 CGI movie despite having been absent from both live action films.
- The Vamp: The Supervisor sometimes resorted to her feminine wiles to get Mortadelo and Filemón to do anything against their will.
Voiced by: Rafael Calvo (1994 series)
- Adapted Out: He's absent from all but one of the comic's adaptations.
- Ascended Extra: He's pretty much a regular character in the Animated Adaptation, as opposed to his use as secondary in the comics, from which he was long gone by the time the animated series premiered. This is a stark contrast to every other adaptation from the comic, in which he isn't featured at all.
- Chuck Cunningham Syndrome: Bestiájez only appeared as a full-fledged character during The '70s and The '80s, after which Ibáñez would use a random Suspiciously Similar Substitute to fulfill his role. However, his surname would be sporadically used over the years in what could be considered re-designed versions of him.
- Bald of Evil: Bald like most male characters, and this time in a much nastier light.
- The Brute: For the Supervisor, who used him to chase and capture Mortadelo and Filemón whenever they ran away during the mission's briefing.
- Identical Stranger: 2011 saw an appearance by a character named Telesforo Bestiájez whose design was identical to the existent agent, only with a different role.
- Limited Wardrobe: Mostly a brown suit, although it could change color.
- Names to Run Away from Really Fast: "Bestiájez" is a fictitious surname created out of the colloquial Spanish word bestiajo, meaning "big beast" or "brutish". It could be translated to English as "Bruteson".
- Put on a Bus: Subverted. He was mentioned to have been fired by the Supervisor in a chapter, although by the next he seemed to be in the organization again.
- Running Gag: Every time he appeared, he would announce from another room that he had finally found Mortadelo and Filemón, though giving an ambiguous clue about where had they run away to. This would be followed by the Supervisor making a believable assumption about it, only for Bestiájez to enter the room dragging them behind him and revealing he meant a much, much far away place (as in other countries or even planets). This gag was inherited by Bestiájez's sucessors.
- Small Role, Big Impact: Despite being just a sporadic character from an ancient timeframe, he is exceptionally well remembered in popular culture, probably because the 1994 animated series adapted several of the chapters where he appeared and made him an ever-present force in the TIA. In fact, considering this was the first, only and last time he appeared in an adaptation (and that he had disappeared from the comics much before), it is a feat that people still remember him so well.
The TIA Chairman
Voiced by: Joaquín Muñoz (1994 series)
- Ambiguous Situation: His portrayal as a bespectacled, mustachioed gentleman only became solid in 1991. Before that, the TIA chairman had a different design in every appearance. It is unknown if this means they were different people in the job or just a huge case of Early Installment Character-Design Difference.
- Badass Mustache: White and two-pointed.
- Bad Boss: Downplayed. While he has proved to be almost as vengeful and abusive as the Supervisor if pressed enough, he is clearly a much more reasonable, collected individual and it takes much more for him to get angry.
- Bald of Awesome: The first straight example, given that he is both bald and a proper gentleman who leads the TIA.
- Informed Attribute: Some chapters state he is from the Canary Islands, mostly to make some pun at it behind his back. However, this is a pretty random piece of info that is never shown on page; the Chairman doesn't even resemble any humorous stereotype of Canarian people as it could be natural to expect.
Played by: Emilio Gavira
Voiced by: Emilio Gavira