Kinjiro "Jiro" Sakamachi
A second year high school student who accidentally discovers Subaru's secret. He suffers gynophobia in which his nose bleeds whenever a girl touches him. He reveals the cause to be his mother and sister practicing their wrestling moves on him on a daily basis for ten years as part of his training. He states his nose bleeds when a girl touches him because his body developed the reflex. He was forced to sign a contract with Kanade Suzutsuki to not reveal the secret of Subaru Konoe in exchange of finding a way to cure his gynophobia.
- Accidental Pervert: This is actually what kickstarts the plot. Jiro caught a sight of Subaru's panties after entering the toilet stall without knocking, and then accidentally groped her chest, discovering her gender. To his bad luck, these kind of incidents don't stop there for him.
- Allergic to Love: He suffers from a condition where he freaks out and gets a nosebleed whenever a girl touches him, to the point of passing out if it gets too bad. His mother and sister are to blame for this.
- Base-Breaking Character: In-Universe, Jiro has unwittingly become this to Subaru's fangirls, on account of his (alleged) gay romance with Subaru. In fact, fangirls who approve of their relationship (implied to constitute roughly half of Subaru's entire fanbase), led by Nakuru, formed their own club, "Let's Warmly Protect Subaru-Sama Committee" (Mimamorugai), leaving the original club, "Shooting Star Subaru-Sama" (S4), entirely to the other half the ones who vehemently object to such a relationship.
- Big Brother Instinct: As much he hated his sister using him as a punching bag, he cares deeply for her, being the only family member with her. One chapter has him diving to save her from an incoming truck and an anime episode has him being there with her on important days.
- Butt-Monkey: Often get teased, embarrassed or beat up by the girls.
- Chick Magnet: A total of four girls are in love with him.
- Embarrassing Nickname: Jiro's full name (in Japanese order) Sakamachi Kinjiro has an unfortunate arrangement such that the last syllable of his surname and the first of his given name can be combined to sound like "chicken", something he really has a major complex with (thus he prefers to be addressed as "Jiro") and called out on by Kanade and later Usami.
- Hilariously Abusive Childhood: From the age of 5, his mother and sister often use him as a punching bag. This went on everyday for the next ten years and what caused him to suffered from gynophobia.
- In-Series Nickname: He's frequently just referred to as Jiro.
- Mistaken for Gay: Usually happens to him regarding his relationship with Subaru, since most people still are unaware that Subaru is a girl.
- Nosebleed: He has one whenever a girl touches him. Unlike most examples, this isn't caused by sexual arousal, but from a reflex he developed to stop his mother and sister from using him as a sandbag.
- Second Year Protagonist: He's the main character of the story and a second year high school student.
- Secret Keeper: He's made promise to not reveal Subaru's true gender to anyone.
- Sweet on Polly Oliver: Downplayed. He falls in love with a girl disguised as a boy, but the romance started after Jiro found out Subaru is a girl.
- Took a Level in Badass: Towards the end of the manga, he undergoes more training to the point where he's able to fight Subaru's father to a draw.
- You Gotta Have Blue Hair: He has dark blue hair.
A student from Jiro's same year who works as a butler for the Suzutsuki family. Subaru is actually a girl who dresses like a male due to family circumstances where one of the family has to serve Kanade's family as a butler. Kanade's father will only allow to maintain her position (and fulfill her family tradition) if she can spend the entire 3 years in high school without anyone discovering her true gender.
- Academic Athlete: She is said to excel both in academics and sports.
- The Ace: She's attractive, intelligent, athletic, popular and a prodigy of great talent.
- Action Girl: Subaru is a very strong fighter and can kick a lot of ass.
- Animal Motifs: Subaru is associated with sheep, because sheep (hitsuji) and butler (shitsuji) sound similar.
- Attractive Bent-Gender: Subaru is thought to be cute either as a girl or a boy.
- Battle Butler: She's Kanade's personal butler and the best fighter in the series.
- Bifauxnen: She can easily pass off as a very pretty-looking boy.
- Birds of a Feather: Both Subaru and Jiro lost one of their parents and have a significant fear that they help each other to overcome.
- Bodyguard Crush: She is an on-off bodyguard of Jirou and falls in love with him after some time.
- Bokukko: She refers to herself with the masculine Japanese pronoun boku.
- Calling Your Attacks: She likes to give what she thinks are cool names to her battle moves. Though Jiro questions Subaru's naming sense.
- Childhood Friends: She has known Kanade since they were kids and Kanade clearly considers Subaru her friend. However, Subaru doesn't like acting too friendly with her because she prioritizes her role as Kanade's butler over being her friend.
- Clingy Jealous Girl: Subaru really doesn't like it whenever she suspects Jiro is too interested in other girls.
- Contralto of Danger: Yuka Iguchi and Genevieve Simmons play Subaru with a low, gutteral voice.
- Dating What Daddy Hates: Her father hates Jiro with a burning passion and it intensifies even further when the two of them become a couple.
- Defrosting Ice Queen: She initially hates Jiro for finding out her secret and thinks that he's a pervert, but she quickly warms up to him and eventually falls in love with him.
- Even the Girls Want Her: Subaru has many female admirers.
- First Girl Wins: In the manga, she is the first girl Jirou meets and would later becomes his girlfriend/fiance.
- Gender-Blender Name: Subaru is a gender neutral name, which is quite convenient for her, given her Sweet Polly Oliver situation.
- Girls Love Stuffed Animals: She's a fan of the popular "Lost Sheep" series of plush dolls.
- Goofy Print Underwear: Subaru's animal-print panties on the two occasions she was exposed cat-print for Jiro, dog-print for Mayoi.
- Hidden Buxom: The Beach Episode in particular shows that she has some "decent material" hidden under her suit than can fill out a swimsuit quite well.
- Idiot Hair: She has a cowlick that stands prominently atop her head.
- Insecure Love Interest: Due to the nature of her situation, Subaru is constantly worried that she either isn't good enough for Jiro, or that he's either dating, or likes someone else. Despite that, Jiro always tries to reassure her.
- Lethal Chef: Her cooking is so terrible that she is not allowed to enter the kitchen.
- Letting Her Hair Down: She lets her hair down when she dresses up like a girl.
- Meido: At the end of the manga, she is forced to resign her position as butler to the Suzutsuki household and takes on the position of maid to the household instead.
- Oh, Crap!: When Jiro discovers her true gender, she is utterly shocked.
- Only Sane Woman: Probably the most level headed among the girls in Jirou's life; Kanade often makes fun of him, his sister beats up him, Usami's Tsundere tendencies has her always scolding him and Nakuru's obsession with glassess and Boy's Love. In fact, Subaru is the only girl Jirou is capable of forming a proper friendship with and is likely the reason he falls in love with her.
- Rapunzel Hair: When she lets it down, her hair reaches down her waist.
- Recursive Crossdressing: On occasions, Subaru disguises as "his" female cousin Puniru.
- Relatively Flimsy Excuse: Subaru sometimes dresses up like a girl, under the identity of "his" own cousin.
- Sarashi: She wears one to flatten her chest when crossdressing.
- She Cleans Up Nicely: She always looks stunning when she dolled up.
- Suppressed Mammaries: Subaru binds her breasts with a chest-wrapping bandage so no one realizes she's a girl.
- Sweet Polly Oliver: She has to disguise herself as male to keep her position as the Suzutsuki family butler.
- Tomboy with a Girly Streak: She spends most of her time disguised as male and is a powerful fighter, but she has a soft spot for stuffed animals especially sheeps.
- Tomboyish Ponytail: She ties her hair in a ponytail when in her male persona.
- Tomboyish Voice: She has a voice so deep it can easily pass as a teenage boy that hasn't broken his voice yet.
- Violently Protective Girlfriend: Don't try messing with Jiro because Subaru will get you.
The daughter of the high school's principal, who appears to behave as a rich man's polite and elegant daughter should, but is revealed to be quite sadistic. She is shown to enjoy tormenting and teasing Jiro by using his gynophobia against him.
- Affectionate Nickname: Subaru calls her "Kana-chan" when she drops the butler act.
- Bi the Way: She develops feelings for Jiro and claims Subaru was her first love.
- Childhood Friends: She has been friends with Subaru since childhood, even if Subaru refrains herself of acting too friendly around Kanade.
- Girlish Pigtails: She wears her hair in twintails.
- I Love You Because I Can't Control You: Kanade fell in love with Jiro because he was the only guy able to talk her down.
- I Want My Beloved to Be Happy: She falls in love with Jiro, but is willing to give up on him so he and Subaru can be together.
- Ojou: She's the daughter of a very wealthy family. Complete with a Big Fancy House.
- Rapunzel Hair: She has waist-length hair.
- The Tease: She just loves to tease Jiro and put him in embarrassing situations. Particularly hilarious during the beach scene in episode 7 when she makes some really erotic noises and suggestive lines as he tries to nonchalantly put sunscreen on her, embarrassing him a lot in the process.
A member of the Shooting Star Subaru-Sama fan club, but quit afterward and is also a member of the handcrafting club. She blackmailed Jiro, with the picture of him and Subaru on a mock date, to be her boyfriend up until the end of their school fair. Jiro accepted because of the condition that she will not spread the photo with Subaru and Kinjirō going out together.
- Animal Motifs: She has a rabbit motif for her name sounds similar to "usagi" ("rabbit"), and for some reason styles her hair in a way that vaguely resembles rabbit ears despite supposedly hating that conflation.
- Comically Missing the Point: Usami thinks Subaru can make for an incredibly sexuality-questioning crossdresser when all dolled up.
- Embarrassing Nickname: Usami's name sounds similar to "usagi" ("rabbit"), a fact that gets made fun of a lot. It doesn't help that she's pretty petite and the ribbons that bind her twintails stick out like bunny ears.
- Girlish Pigtails: As it's typical with Tsunderes, she ties her hair in twintails.
- Interrupted Suicide: In the manga, Usami nearly kills herself due to her inability to trust anyone finally getting too much for her. Fortunately, Jiro is able to stop her.
- Meido: In the manga, she becomes a maid for the Suzutsuki household.
- Tsundere: Down to the twintails and thigh-high boots. Lampshaded by Kanade in episode 9.
- Weirdness Magnet: She's able to attract ghosts if not living together with them perfectly, such as ghosts appearing in her photos and randomly popping out in her apartment.
- You Gotta Have Blue Hair: She has lavender hair.
- Zettai Ryouiki: She wears thigh high boots, making her a rare Grade S.
Jiro's younger sister who is a first year in high school and loves martial arts. She is a member of the handcrafting club. Still believing Subaru to be male, she falls in love with Subaru, much to Jiro's chagrin.
- A-Cup Angst: She's self concern of her bust size when compared to the other girls.
- All Amazons Want Hercules: Kureha wants a man who's stronger than her.
- Annoying Younger Sibling: Especially because she likes practice her wrestling moves on her brother.
- Best Her to Bed Her: She develops a crush on Subaru after she easily defeats her in a fight, unaware that Subaru is really a girl.
- Pintsized Powerhouse: Despite her size, she can take on a bear and only receives a broken arm.
- Rose-Haired Sweetie: She's cheerful and energetic and has pink hair.
- You Gotta Have Blue Hair: She has pink hair.
A busty first year and a member of the crafts club along with Kureha, she is also revealed to be the chairman of the "Let's warmly protect Subaru-Sama committee" and is shown to have a fetish for glasses and boy love which is expressed through her manga drawing of Jiro and Subaru, much to their chagrin.