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The world-famous inventor who created the Elaborate Underground Base inside Mystery Mountain, which serves as the game's setting. He is away on a business trip for the entire game.
- All There in the Manual: According to the old Knowledge Adventure website, his full name is Professor Alberto Leonardo Sparks. (The website used the "Sparks" spelling, see Sudden Name Change.)
- Deus Exit Machina: Apparently, he would be able to control Polly if he was around, but he's not. He returns home at the end of the game.
- Omnidisciplinary Scientist: Judging from his inventions, he's involved in just about every field of scientific research plus, based on the Art Gallery and Music Hall, the humanities.
- Reasonable Authority Figure: Botley clearly respects his authority, and reasons that he'd understand Botley breaking a few of his rules for the greater cause of saving the world. And when he does return home at the end of the game, Professor Spark quickly sees through Polly's attempt to blame everything on Botley.
- Sudden Name Change: His last name is "Spark" in JumpStart 3rd Grade, but it changes to "Sparks" in JumpStart Typing.
Professor Spark's third-grade daughter. After failing a history quiz at school, she decides to send twenty-five robots back in time to change history so that her silly quiz answers will be correct. She is the game's villain, and your task is to foil her plans.
- Big Bad: She is the overall villain of the game, and is responsible for the whole time-travel plot that you have to stop.
- Book Dumb: Sort of. She doesnt seem to do well in quizzes.
- Dub Name Change: She's named "Klara Blixt" in the Swedish release of the game.
- Enfant Terrible: Polly is a malicious kid.
- HeelFace Turn: Polly mellows out considerably by the time of JumpStart Typing, with her and Botley on better terms.
- Four-Fingered Hands: As seen in the image on the right.
- Spoiled Brat: She has a very rotten and entitled attitude. How she got so spoiled is a bit of a mystery since her father doesn't seem especially doting.
- Sudden Name Change: As with her father, her last name becomes "Sparks" in JumpStart Typing.
The teacher of Polly's third-grade class. She runs a Raygun Gothic version of a traditional one-room schoolhouse.
- Schoolmarm: Technically, since she does run a one-room schoolhouse, albeit a Space Age version of one.
Botley (Android XL-2)
Your helpful guide and companion. He's meant to be Polly's babysitter, but the emergency situation means he has to instead devote his efforts to correcting the damage Polly has done to history.
- Badly Battered Babysitter: Botley was created for the onerous duty of babysitting Polly because she kept scaring away the sitters hired by her father.
- Dub Name Change: His name is "Roybot" in the Swedish release of the game.
- Exposition Fairy: He serves as your guide throughout the game.
- Nice Guy: Botley is nice to literally everyone in the game.
- Robot Buddy: He's meant to be one for Polly, although she apparently isn't going for it. He is your buddy, however.
A very hungry robot who has locked himself in the Mountain's kitchen. You must satisfy him in the Robot Kitchen mini-game in order to receive a mission clue or some Invention Points.
- Big Eater: Botley notes that Mort eats so much that other robots call him, "the bottomless pit." It's true enough that Mort declares that he's starving every time you visit the kitchen.
- Eating Machine: The ingredients for Mort's meals include a mix of human food, stereotypical robot food like gears, and just plain random items such as soap and gold bullion.
- Top-Heavy Guy: His arms are bigger than his legs.
The archivist of the Art Gallery.
- Beehive Hairdo: She has a blue, beehive-like hairstyle.
- Scary Librarian: Subverted; she may have this vibe, but she is ultimately friendly.
- The Smurfette Principle: Of all the robots to preside over a mini-game, she is the only Fembot
A robot head who serves as an animated door knocker for the entrance to the Music Hall.
- I Can't Hear You: Considering who he's based on, it's inevitable.
- No Celebrities Were Harmed: Well, if you would categorize Ludwig van Beethoven as a celebrity anyway.
- Punny Name: Do we even need to explain it?
The robot maestro who runs the Music Hall. He speaks with an Italian accent.
- One-Wheeled Wonder: As seen in the image on the right
- Transforming Mecha: Can transform into a cello if it is selected by the player.
A robot scientist who presides over the Shrinking Machine Room and serves as caretaker for a number of specimens in there.
- Friend to All Living Things: Including bugs, and is offended when Polly makes mean-spirited jokes about his beloved specimens.
The talking monitor who presents the quiz you must pass in order to access the Time Machine.
- Game Show Host: Since Polly has reprogrammed his quiz into being a Game Show called "Pollywood Squares", Monty now has the persona of one.
A robot who resembles a little hovering shark and apparently serves as Polly's minion.
- No Name Given: Polly's robot is never referred to by name.
- The Quiet One: This robot never talks, at least not on-screen, but does occasionally let out a mischievous cackle.
The time-traveling robots
The twenty-five robots Polly sent back in time. They include, in the order depicted in the image at right:
- Top row:
- Second row down:
- Danny Defrost-Bot: A snowman-themed robot
- Roland Road-Bot: A track marshal-themed robot
- Chef Boy-Robot: The robot chef
- Verna the Vend-Bot: A vending-machine robot
- Brunwella the Bombshell: A demolition robot whom Botley evidently has a crush on
- Pierrot-Bot: A Pierrot-themed robot clown artist
- Bongo-Bot the Six-Armed Robot Drummer: A, um, six-armed robot drummer
- Vasco da Robot: A Bold Explorer who is pen-pals with Botley
- Dr. Bug-Bot: The robot doctor
- Third row down:
- Slide the Heavy Metal Robot: A literally heavy metal robot (he's made of lead) whom Polly reprogrammed to be a rock star
- Noshi Origami: A exchange-student robot from Japan
- Eraser-Bot: A pencil-themed robot
- Amelia Air-Bot: A pilot-themed robot
- Roast-Bot: A robot fireman
- Bubble-Bot: A bubble-themed robot who was programmed to walk and chew gum at the same time ("That may not sound like much, but it took a long time to perfect")
- Bottom row: