Formerly an agave farmer with the dream of being a Luchador, Juan was killed by Calaca on his way to the nearby town of Pueblucho. He was restored back to life by a mystical mask and began his adventure to save El Presidente's Daughter and defeat Calaca. Juan is the "default" luchador the player starts out with, but can switch to Tostada by changing costumes.
- Action Dad: In 2 he and Lupita have two kids, and he will do anything to protect them.
- Badass Mustache: Juan sports one under his mask.
- Baleful Polymorph: Calaca turns him into a chicken but this later is fixed.
- Formerly Fit: After seven years of inaction, Juan gained a lot of weight, but he gets back in shape after putting on the Mask again.
- Heroic Mime: Juan is never shown talking, but the dialog of other characters seem to indicate that he is indeed communicating with them.
- Masked Luchador: Juan doesn't begin the game as one, but quickly comes across a mystical mask and becomes one.
- Morality Chain: To Jaguar Javier in the sequel, due to being the only Juan that survived fighting Calaca the main timeline Javier is the only one not to work for Salvador.
- Voluntary Shapeshifter: It turns out being a chicken has a lot of advantages, primarily for gaining access to tight areas Juan cannot craw through.
The spirit of the Lucha mask that brought Juan back to life, she aids Juan on his adventure. By default she's Player 2 but Player 1 can be her by changing costumes.
- The Faceless: La Tostada wears her luchador mask all the time, even when she's wearing a dress.
- Famed In-Story: Added in STCE, she's hailed in the town of Canal de Flores as a legendary heroine who gave her life saving the people from erupting volcano.
- Kick Chick: La Tostada's Stamina Attacks use kicks instead of punches like Juan.
El Presidente's Daughter/Lupita
Juan's life long friend and love interest, though they haven't seen each other for years. Calaca had captured her because she was born on a day of a special eclipse which made her the perfect sacrifice and bride.
- Distressed Damsel: Rescuing her is the goal of the game.
- Everyone Calls Him "Barkeep": In the first game, she is referred to solely as "El Presidente's Daughter". Subverted in the second, where she's just addressed by her name.
- Happily Married: In 2, she and Juan are married with two children.
- Virgin Sacrifice: Calaca's plan for kidnapping her. Depending on the endings, she ends up getting saved.
Uay Chivo, the Goat Hermit and self-proclaimed "lord of all goat-men", is cantankerous old man with mystic powers and (previous) owner of several "Choozo" statues. He teaches Juan and Tostada new moves whenever they bust up one his statues, typically named after animals (with a few exceptions).
- Alliance of Alternates: In the sequel he has contact with versions of himself from alternate timelines.
- Dirty Old Man: He likes to ask about Juan's mother a couple of times and asks if she likes "baaaaaaaad" boys
- Expy: Of Cranky Kong, being an hunched over old man with an attitude and a big walking stick.
- Promoted to Playable: Becomes a playable character in Guacamelee! 2.
- Tranquil Fury: Upon learning that Javier became a villain to protect him from Calaca, the Hermit quietly asks Juan to sock the latter across the face for him.
- Voluntary Shapeshifter: He's a Huay Chivo, a Mayan sorcerer who can transform into a goat (among other things) and attack livestock. It's why he wears Mayan clothing.
- A God Am I: He wishes to merge the worlds of living and dead and rule it as an omnipotent god.
- Big Bad: He is the main villain of the first game.
- Freudian Excuse: Carlos Calaca's turn to villainy came when, on the eve of the Charreada, he broke his arm and thus made a Deal with the Devil to mend it. But before he could claim his prize, the Devil dragged him down to hell. A vengeful Carlos plotted to overthrow the Devil, and then merge the worlds of the living and the dead just so he could get the praise that was owed to him.
- Glowing Eyes of Doom: Carlos' undead skeleton form has intense, burning red eyes.
- One-Winged Angel: Upon completing the ritual, he transforms into a gigantic red demon.
- Red and Black and Evil All Over: His black charro outfit, along with his reddish features (eyes, mustaches, and eyebrows), invokes this image.
- The Worf Effect: In the sequel, Salvador's threat level is highlighted by the fact that in an alternate timeline, Salvador was the one to defeat Calaca, not Juan.
- Ambition Is Evil: Salvador's descent to darkness started when he forcibly took the Mask of Power to become the greatest luchador ever, despite his mentor/parent figure's warning that he was not ready.
- Animal Motifs: Snakes. He is referred to as "Salvador the Snake", his hideout is the Temple of the Snake, and his mask gives him the power to summon ethereal snake familiars as well as actual snakes
- Battle Aura: He gains reddish glow once he eats Sacred Guacamole to cure his illness. His final form ditches this in favor of the snake familiars that support him, however.
- Big Bad: In the sequel.
- Blatant Lies: He insists that he isn't actually sick, coming up with various unconvincing excuses every time he coughs. One such excuse ends up being his Famous Last Words.
- Contrasting Sequel Antagonist: Unlike Carlos Calaca, he's a luchador, making him a straightforward Evil Counterpart to Juan.
- Drunk with Power: His former parent figure states that he is consumed by the power of the mask.
- Evil Counterpart: Like Juan, he aspired to be a luchador and wears a magic mask, but didn't have the same strong sense of justice Juan had, and the power of the mask (according to his parental figure) corrupted him.
- Evil Gloating: A Running Gag is him trying to do this, only to be interrupted.
- Evil Is Bigger: Despite being a normal human he's positively huge compared to Juan.
- Fallen Hero: He saved the Darkest Timeline from Calaca, but became evil afterwards.
- Find the Cure!: His actual plan.
- Glowing Eyes of Doom: Has glowing eyes, which is likely due to the power of his mask as they return to normal when it gets destroyed.
- Heel: Is basically the rudo to Juan's técnico.
- Incurable Cough of Death: Curing it is his motivation for his villainous deeds. Not that he'll ever admit to having it in the first place.
- Mask of Power: Just like Juan, Salvador gained great powers through the mask he wears.
- No Man of Woman Born: He starts the second phase of the fight by using a shield that, as he points out, no human can break, cue chicken form.
- Not So Different: Compares himself to Juan in that they both started from nothing to become great Luchadores, however he believes Juan wasted his potential.
- Pet the Dog: Asks himself at one point if he was being too harsh on Muñeco after snapping at him for constantly interrupting him, showing that he does care for his underlings to an extent.
- Power Degeneration: His sickness is due to his body being unable to handle the power of the mask.
- Time Master: A side effect of his plan, if collecting the relics hasn't made him one already the cure probably will.
- Villainous RROD: He became terminally ill trying to become the greatest luchador ever, and his plan is to find a cure for his condition.
- Affably Evil: Violent psychopath that he may be, he can also be laid back and easygoing. He doesn't even hold any grudges with Juan in the sequel and just challenges him to get better at performing combos.
- The Alcoholic: If Flame Face doesn't have his guns drawn, he's usually got a bottle of tequila in one hand and a glass in the other. Oddly, he usually finishes off a drink by swallowing the glass itself.
- Combos: In 2, his skill tree focuses on building and extending combos.
- Cursed with Awesome: In life, Flame Face used to set people on fire, thinking it would curry favor with the Devil. Upon death, the Devil instead cursed him with an eternally flaming head. When you meet him in the sequel he explains that he survived because the "eternally" part was literal.
- The Gunslinger: He is the only character who uses guns and dresses up like a cowboy.
- HeelFace Turn: Goes from a boss character to a trainer in Guacamelee! 2.
- The Hyena: Flame Face likes to laugh hysterically while firing his guns (unfortunately, this frequently leads to him running out of bullets when it comes time to use them more practically.)
- Pyromaniac: Appears to be this, if his desire to roast El Presidente's daughter is anything to go by.
- Femme Fatale: Initially tries to invoke this, but acting seductive and speaking Double Entendre when she meets Juan.
- High-HeelFace Turn: After being defeatead, she helps out the heroes by granting them the power to cross the two dimensions at will.
- Me's a Crowd: Can create a duplicate of herself to fight alongside her.
- Promoted to Playable: She's a playable character in Guacamelee! 2.
- Rapunzel Hair: X'tabay's flowing locks. It's a terrifying sight when her fury makes them stand on end.
- Cowardly Lion: A recurring problem for him and his alternates, Javier may be a badass, but he is very easily intimidated and loses hope when it looks like the bad guys are winning, making it really easy to talk him into a FaceHeel Turn.
- The Dragon: He is the strongest of the Quirky Miniboss Squad and the second-to-last of the bosses.
- HeelFace Turn: The epilogue shows that after the events of game, he became friends with Juan, same goes for the his alternates in the sequel.
- Mirror Boss: He fights using moves similar to the players.
- Noble Demon: He admires your fighting spirit and refusal to bow. played with the Alternate Javiers, while they do regain hope after seeing Juan in action they're willing to gang up on him in a boss fight.
- A Pupil of Mine, Until He Turned to Evil: He was the former apprentice of the Goat Hermit. As it turns out, he serves Calaca in order to protect his mentor, his alternates in the sequel however don't even need the threat of a hostage to be intimidated into serving Salvador.
- Wolfpack Boss: In the sequel, he summons two more alternates of himself during the boss fight.
El Trio De La Muerte
- Freudian Excuse: They want to find their long-lost band member.
- Multiple Head Case: They're three undead mariachi members who share a body.
- Obliviously Evil: In the sequel, they do not realize that practicing their music near the volcano is causing it to erupt and thus have to be stopped by Juan.
- Remember the New Guy?: Ultimately subverted, but lightly poked fun at in STCE. Their first appearance is just after Juan is killed by Calaca, with one of them asking if they missed anything as the scene fades to black.
- Take That!: They're essentially a spoof of failed crowdfunding project, trying to pitch their flawed prototype soldiers to Calaca with expected results.
- Terrible Trio: They are Mexican mariachi version of this. In fact, their reason for helping Calaca is to revive their fourth band member and get out of this status.
- Evil Counterpart: To the Goat Hermit. Just as the Goat Hermit helps guide the heroes to defeat evil, she helps Salvador achieve his evil ambition. They also used to date.
- Flunky Boss: While other antagonists summon mooks in their boss fights, she stands out as defeating all the mooks she summons is mandatory as part of her boss battle.
- Hoist by His Own Petard: In the final phase of her boss fight, she transforms into an invincible abomination and proceeds to eat Juan, which allows him to defeat her by punching out her heart.
- King Mook: Design-wise she is this to the Shamans, although unlike them she cannot buff other enemies and opts instead to blast you with offensive magic while you're distracted by her mooks.
- Losing Your Head: All that's left of her after her boss fight is her skull.
- Our Liches Are Different: An undead skeleton dog-woman who "command[s] the darkest canine sorceries".
- Zero-Effort Boss: After her initial boss fight she has her skull put onto a Giant's body and challenges you to a rematch right before you fight Salvador. She goes down in one hit.Uay Pek: "Become a Giant, Uay Pek! It'll be great!" That's the last time I listen to Muñeco.
Zope y Cactuardo
- Alas, Poor Villain: In the ending montage they attend Salvador's funeral.
- Attack Its Weak Point: To damage Cactuardo you need to knock Zope off his head first.
- Brains and Brawn: Zope serves as the brain while Cactuardo provides the muscle.
- Dual Boss: Though they mostly take turns.
- Fragile Speedster: Zope.
- Mighty Glacier: Cactuardo.
- Mirror Boss: Zope can use the Pollo Shot and Pollo Slide, although he can also fly and throw knives. Justified as he isn't a chicken.
- Not in This for Your Revolution: Zope doesn't really care about Salvador's goals, he just hates the chickens, and since Salvador's plan involves stealing their relic, he's in.
- The Unintelligible: Cactuardo, with Zope acting as a translator.
- Ambiguously Gay: He certainly is very flamboyant (although that is pretty much a prerequisite for his career as a stage performer regardless of orientation), and his entire goal is to become Salvador's "best friend", but nothing is explicitly stated.
- Animal Companion: He has several chickens that act as assistants during his magic shows, and in combat he uses them as projectiles in a "pull a rabbit out of my hat" trick.
- Boss Arena Idiocy: There two spiked pillars that spring up when the pressure plates on the ground are pressed. These can be used to knock Muñeco out of his tornado attack and to block his chicken barrage attack.
- Bunny-Ears Lawyer: Despite his kooky personality he's quite competent as a supervillain. After you beat him in a boss fight he reveals that he was just stalling you while his chickens stole the Jade Relic (although it turned out to be a decoy), and when he and Salvador arrive at the Obsidian Temple his first reaction is to suggest distracting you so Salvador can sneak attack you.
- Everything's Better with Spinning: His masked tornado attack.
- Evil Laugh: Does this all the time. In the boss fight, this tends to provide Juan with the opportunity to reach and hit him.
- Expressive Mask: His theatre mask will change expression to reflect his emotions.
- I Just Want to Have Friends: His entire motivation for serving Salvador is to become his best friend forever.
- Magicians Are Wizards: He's a stage magician and uses magic tricks in combat.
- Malevolent Masked Men: Wears a theatre mask with a beauty mark.