Throttle is the tan one, a quiet and decisive natural leader who cares deeply for those under (and over) his command- even though he may be too manly (or mousely) to show it. He was groomed by Stoker, the creator and former leader of the Freedom Fighters, to take command- but something interrupted it, and he ended up in space with Vinnie and Modo. Curiously enough, he's the only one who's displayed the Martian skill of telepathy, and telepathic trickery as well. He was blinded in the Mars-Plutark war, and courtesy of Dr. Karbunkle now has semi-working replacement eyes that he needs his signature pair of sunglasses to see with.
Tropes associated with Throttle:
- Berserk Button: Don't hurt his bros, if you know what is good for you.
- Blind Without Them: If he is not wearing his field spec or his helmet all he can see is a field of white.
- Cool Shades: He wears a pair of shades straight out of the 90s fashion mags.
- Sunglasses at Night: Because he needs them to see, he almost never takes them off.
- Covert Pervert: He suggested to go see a mud wrestling competition for Charley's birthday.
- He was also happily ogling Carbine in a bathrobe.
- Deadpan Snarker: For example:Vinnie: Yeah! Some pretty studly moves there!Modo: Well, that's pretty nice of you to say, Vinnie.Vinnie: Not you, bro. I was talking about me!Throttle: What a surprise.
- Handicapped Badass: His vision is severely impaired without his sunglasses, thanks to Dr. Karbunkle.
- Lawful Stupid: He once stopped chasing Limburger due to a red light.
- The Leader: He tends to call the shots. He averts being a Standardized Leader because he can be just as laid back and wild as his bros.
- Megaton Punch: The Nuke Knucks Harley gave him allows him to demolish a tank with a single blow.
- Mr. Exposition: Is usually the one to explain or show Charley past events.
- Official Couple: With Carbine. Living on different planets puts quite a strain on the relationship, however.
- The Stoic: Usually the most level-headed protagonist.
- Not So Stoic: Unless he decides not to be.
- The Strategist: He is the one who comes up with all the plans. Stoker was even grooming him to take his place as the leader of the Freedom Fighters.
- Touch Telepathy: Apparently every Martian mouse can share thoughts by making contact with other people, but Throttle is the only one we see.
- You Called Me "X"; It Must Be Serious: He tends to call Vinnie "Vincent" when he wants the white mouse to really listen to him.
He's the self-proclaimed baddest biker this side of ten solar systems, and he's almost right. Vinnie has a huge ego that was only outdone once in the whole series, but underneath his bluster lies a sweet, caring individual who would sacrifice anything for his bros and Charley. He gained his mask in the Mars-Plutark war, either by being blasted at or by a sadistic experiment by Dr. Karbunkle. Either way, his first love Harley (no relation to Charley) gave him a mask made out of flex-plate metal, which allows his face to move while still covering up his scars.
Tropes associated with Vinnie:
- Always Save the Girl: He is the most protective of Charley, and usually the one that come to her rescue. Not being able to do it for Harley is an Old Shame for him.
- Arrow Catch: Does it with his teeth in "Once Upon a Time On Mars".
- Badass Bandolier: Wears two green bandoliers, crossed over his chest in an X.
- Badass Biker : All the Biker Mice are this, but his rashness put him above the rest. He proved it in "The Motocross Trap", where he won a race against his bros, the Lougie Brothers and Evil Eye Weevile.
- Berserk Button: Never hurt or threaten Charley in his presence. It's implied he was about to kill Limburger when he framed Charley for murder (Throttle had to remind him they needed Limburger to free her).
- Big Eater: One View-Master frame Limburger looks at has Vinnie stuffing his face with hot dogs.
- Blood Knight: A rare heroic example. A week without fighting Limburger's goons reduced him to an angry, nervous wreck who had to punch through a brick wall (repeatedly) to try to calm himself.
- Boisterous Bruiser: Vinnie's major defining trait, after his massive ego.Vinnie: Yep, that's me! I'm the baddest motorcycle mamma-jamma in the universe!Throttle: Modest, too.
- Butt-Monkey: While he is a great biker and a good fighter he also has the biggest ego, which tends to blow up in his face more often than not.
- The sequel unfortunatly took this Up to Eleven.
- Casanova Wannabe: He likes to declare himself a suave lady-killer, but not only most of his flirting backfires, but he also become a shy mess when the lady seems to return his attention.
- Chivalrous Pervert: Vinnie can hardly keep his mouth shut around a pretty woman, but he's also protective over the women in his life (Harley and Charley being the main examples).
- Expressive Mask: The metal plate over his face doesn't keep him from having a decent range of facial expressions.
- Fearless Fool: Driving with a bomb sets to explode in 30 seconds attached to his bike? Why, Charley, you spoil him.
- Handsome Lech: Despite the flex-steel, he's quite handsome and doesn't mind taking advantage of it.
- Heartbroken Badass: In the 2006 revival, his girlfriend Harley went through a FaceHeel Turn since the last time they met and she appears to get killed shortly after she comes to her senses.
- Jerk with a Heart of Gold: He loves to brag about himself, is Innocently Insensitive (especially with his "compliments " towards Charlie) and his foot may as well live permanently in his mouth. As Charley said, he is also "a sweet,kind and gentle mouse" who would never hurt his friends.
- Has a Type: Both the women he fancied were brave, witty, Wrench Wench / Gadgeteer Genius and helped him and his bros in the fight with the Plutarkians.
- Hidden Depths: He is a good Chinese Checkers player, beating Throttle and making Modo Rage Quit.
- Inferiority Superiority Complex: Yes, really. It was most obvious on Mars, where for all his bravado he was clearly feeling inferior to Stoker, both in riding and fighting skills and his possibilities with Harley.
- Informed Flaw: He is considered kind of a womanizer, yet we have only seen him interested in two girls.
- Innocently Insensitive: He honestly trying to compliment Charley by assuring her she has "trophy value" to him is just one example.
- Interspecies Romance: He is not shy about his interest in Charley. She usually playfully rebuff him usually.
- Leeroy Jenkins: Well, all three mice fit, but he's the worst of them.
- Love Triangle: He was involved in one back on Mars, where he and Stoker were both interested in Harley. Harley chose him then, roughly five second later, she was kidnapped.
- Luminescent Blush: Each time Harley or Charley are affectionate towards him. Of course you shouldn't be able to see it with his fur, but
- One-Man Army: He raided the Pits and wrecked their defences to save his bros.
- Only Known by Their Nickname: While he is commonly referred to as "Vinnie", his real name is Vincent.
- Rage Against the Reflection: After a blast on Mars took off half of his face, he took a look in the rearview mirror: seeing the bandages that covered the wound, he pulls it from the bike and throws it away.
- Red Ones Go Faster: His bike is red and speedier and the bikes of Throttle and Modo.
- Signature Laugh: A rapid,hearty one.Vinnie: Ahahahahow!
- The Fighting Narcissist: He is a great fighter and has a high opinion of his look. Losing half of his face in "Once Upon a Time On Mars" was a huge blow to his ego, and he tried to hide it from his crush Harley. She fixed it by giving him the metal mask he is still using.
- Thrill Seeker: A day where he doesn't risk his life is a waste, as far as he is concerned.'''Imminent destruction! What a rush!"
- Walking Shirtless Scene: His two bandoliers are usually the only thing on his chest.
- Weapon of Choice: His flares: they light up like a match and can cut almost every metal, except plutarkian steel.
- Why Did It Have to Be Snakes?: It is revealed in the 2006 revival episode "Biker Mice Down Under" that he is afraid of bats.
- Yank the Dog's Chain : After losing half of his face in a blast and barely saving Mars for becoming Plutark's new satellyte, the girl she liked chose him and then she got immediatly kidnapped.
Modo is a Gentle Giant through and through, until you anger him- in which case he will bring the Unstoppable Rage like a fuzzy grey tornado. His mother imbued him with a philosophy about children, women and life in general that still guides him today, and he quotes her often. Out of the three Biker Mice, he sustained the most severe injuries- losing his right arm and left eye. Again, "courtesy" of Karbunkle he now has a mechanical arm to replace his blown-to-bits one. Unfortunately for him, the arm wasn't really wanted, and Modo still has self-doubts about it to this day.
Tropes associated with Modo:
- Arm Cannon: Modo has a retractable cannon on his mechanical arm.
- Badass Baritone: He has the deepest voice of the three Biker Mice.
- Berserk Button: Don't hit the ladies, the children, or God help you, his beloved bike. Or you will perish painfully.
- And don't even think about hurting his friends, or calling them (or him, for that matter) rats.
- Beware the Nice Ones: He may have a soft heart, but pissing him off can still be hazardous for your health.
- The Big Guy: He is the toughest of the group, and the tallest at a staggering six feet nine inches.
- Catchphrase: Whenever he brings up advice and wisdom he learned from his mother, he frequently starts it with "Like my gray-furred mama used to say..."
- Eyepatch of Power: He wears an eyepatch to cover the eye he lost to Karbunkle experimenting on him.
- Friend to All Children: Modo is extremely fond of children, especially his nephew Rimfire and niece Primer. This trait is used against him once by Limburger to break his self-esteem.
- Handicapped Badass: He is missing an eye and has a robotic arm.
- Heroic BSoD: "Modo Hangs It Up" has him visit a hospital where a boy is hurt as a result of a battle against Limburger. It takes a lot of fight out of Modo. The boy, however, reveals he's really Marshall, Limburger's nephew, and really not hurt at all. In the follow-up fight, Marshall's got a Biker Mice pennant to cheer them on with.
- Momma's Boy: He loves his mother very much and frequently quotes her advice.
- 10-Minute Retirement: In "Modo Hangs It Up", he feels unable to go on with fighting Limburger's minions because of Limburger's nephew Marshall deceiving him by disguising himself as a human child and pretending to be injured in the crossfire. Modo gets over his funk after Marshall reveals that he isn't hurt and helps Modo ruin his uncle's scheme to spite him.
- Unstoppable Rage:
- You know how Modo's eye glows red when he's pissed off? Well, when you threaten his bike, or his bros, or, God help you, both, his entire body glows red (actually, that only happened once, but whatever) and he'll sucker-punch you into freakin' Lake Michigan.
- He and his bros are mice. Not rats. Run away fast if you hear anyone calling them rats when Modo's around.
- Once in a Christmas Episode, given Limburger sunk to a new low by targeting the orphans the Biker Mice were with earlier (and taking out their orphanage), Modo's not pulling punches.
- Why Did It Have to Be Snakes?: It is revealed in the 2006 revival episode "Biker Mice Down Under" that he is claustrophobic.
Charlene "Charley" Davidson
Charlene is a long-suffering woman, considering who she has daily upon her doorstep- either three rambunctious bikers who are experts at accidentally wrecking everything around them, or money-hungry Plutarkians who would like her to be out of their way permanently. Despite both of these, however, she's still alive and kicking, partially due to her own mechanical genius. She's responsible for fixing the Biker Mice's bikes, installing upgrades and treating their wounds. There is no overstating her value to the Biker Mice's cause; if she was not there to make an emergency fix or fashion a new weapon, the Biker Mice would be long dead by now. They know it too, which is why every time Charley declares that she's going along, she's vetoed by all three of the Mice. She still gets her shots in every once in a while, but not by the Biker Mice's choice.
Tropes associated with Charley:
- Action Girl: While the Biker Mice often forbid her from doing so, she's kicked her fair share of butt.
- Amicable Exes: She is still friends with Jack, and was even extremly worried when he was kidnapped.
- Beyond the Impossible: She repaired Karbunkle's Time Machine in less time it takes for a bomb to explode and the bomb was set for ten seconds.
- Badass Biker: She proved this in "The Masked Motorcyclist"
- Boyish Short Hair: Her hair is a lot shorter in the 2006 series.
- Brainy Brunette: She has brown hair and at least has more common sense than the Biker Mice.
- Clingy Jealous Girl: She usually rebuffs or ignores Vinnie's advances, but seemingly didn't appeaciate him wondering if Hard Rock's girlfriend Darla had a sister."Oooooh, men!" ( punches him in the arm, then turns and pouts ).
- Damsel in Distress: The second thing Charley does is get captured and then put in a giant trap so Grease Pit can blackmail the mice into going away. She then yells out "NO DON'T IT'S A TRAAAAAAP!" To her credit, she is painfully aware of this, and tries to avoid it while still being a useful friend to the Mice.
- Deadpan Snarker: Most frequently makes remarks about the Biker Mice not being responsible and rushing into battle without a strategy.
- Gadgeteer Genius: She is much, much smarter than a regular mechanic: her feats include understanding and repairing alien technology far above Earth is capable for, as well as inventing new weapons when needed, like the Sonic Spear in "Mad Scientist Wanted".
- Only Sane Woman: The Mice have hilariously huge egos. She doesn't.
- Punny Name: Her name is a play on the Harley Davidson motorcycle brand.
- Spy Catsuit: Sort of, in "The Masked Motorcyclist", the episode where Charley is testing out an experimental bike while wearing a really sexy catsuit and a helmet that completely obscures her face. The Biker Mice have zero idea it's her until almost the end, when the bad guys shoot at her bike, and it crashes, with her subsequently removing her helmet to check for damage and revealing the sexy mechanic, much to the Mice's total surprise...and PLEASURE.
- The outfit makes a return in "Biker Knights of the Round Table".
- Tomboyish Name: Her name sounds like "Charlie", which is usually a masculine name.
- Women Are Wiser: Considering the mice, that's not hard.
- Wrench Wench: She repairs the mice's bikes.
Sweet Georgie Brown
A radio DJ that the Biker Mice listen to in several episodes.
Tropes associated with Sweet Georgie Brown:
- Mr. Exposition: He tends to conveniently inform the Biker Mice of whatever Lawrence Limburger is up to whenever they're listening to his station.
- Surfer Dude: He talks like a Californian surfer.
- The Voice: He's only heard from the radio. The one time he's seen as a physical person is when Limburger takes over his station and has him bound and gagged in the episode "Vicious Cycles", but even then his face is obscured by a brown paper bag.
A former mercenary for the Plutarkians who now just wants to go straight.
Tropes associated with Hard Rock:
- Amazing Technicolor Population: His skin is green.
- But for Me, It Was Tuesday: When the Biker Mice confront him over the destruction he's done to Mars, Hard Rock replies that he's wrecked too many places to remember.
- Embarrassing First Name: His girlfriend Darla reveals that his first name is actually Booboo.
- HeelFace Turn: He used to lay waste to other planets on behalf of Plutark, but is now a good guy and even helps the Biker Mice thwart Limburger's latest plan.
- I Have Your Wife: Limburger forced him to do his bidding by holding his girlfriend Darla hostage.
- Love Redeems: He went straight for Darla's sake.
- Musical Assassin: His weapon is an electric guitar that fires energy blasts.
- Rotten Rock & Roll: Subverted. He is patterned after a rock musician and he does use an electric guitar as his weapon, but he's a changed man and is only working for Limburger because he's been coerced.
- You Gotta Have Blue Hair: His hair is pink.
Hard Rock's girlfriend.
Tropes associated with Darla:
- Amazing Technicolor Population: Her skin is pale blue.
- Badass in Distress: Initially presented as just Hard rock's Damsel in Distress girlfriend. Then it's revealed she's got powerful telekinetic abilities and singlehandedly takes out Limburger's goons at the episode's climax.
- Mind over Matter: Darla is a powerful telekinetic, who can move things with her mind. At the episode's conclusion, with Limburger's teleporter destroyed, she and Hard Rock return to space through the simple process of Darla mentally lifting up their Flying Car and propelling it out of orbit and through space with her mind.
- More Deadly Than the Male: Hard Rock's no push over himself, but Darla's not the Damsel in Distress she initially appears to be, either, courtesy of her mental blasts.
- You Gotta Have Blue Hair: She has literal blue hair.
A decent guy who was at one point framed for damages Limburger caused to a school. In addition to having his name cleared, he also devotes himself to enforcing the law in the Pit Boss's turf.
Tropes associated with Four-By:
- Arch-Enemy: After ending in the pits he becomes this to the Pit Boss, starting a rebellion against him and swearing to bring him to justice.
- Badass Baritone: He's voiced by Michael Dorn. Of course he'd have an impressive deep voice.
- Berserk Button: Putting people in chains: of course that makes him Pit Boss's enemy.
- Clear My Name: Limburger accused him of attacking his excavator to save himself from the charge of putting children in danger. Thankfully, Charley recorded everything and proved his innocence.
- Cool Car: His Mighty Mo, a big and really durable monster truck. When it gets damaged by the fall into the pits, he rebuild it even bigger.
- Chuck Cunningham Syndrome: He doesn't appear in the 2006 revival. In spite of his dedication to bringing the Pit Boss to justice, he doesn't even appear in the Pit Boss's episode "It's the Pits".
- Gentle Giant: He is one of the tallest and strongest characters, and his Establishing Character Moment is saving Modo and stopping Limburger's machine from tearing up a school.
- Rebel Leader: He start a rebellion in the pits against the Pit Boss.
- Shirtless Scene: A fair amount of his screentime in his debut episode has him bare-chested.
- Why Did It Have to Be Snakes?: He is claustrophobic; it's the reason why he drives such a big car.
The legendary King Arthur, whom the Biker Mice encountered when Limburger tried to obtain deposits of pure tin by time-traveling to medieval times and forcing King Arthur to sell Camelot.
Tropes associated with King Arthur:
- Badass Boast / Shut Up, Hannibal!: When Limburger is about to blow up Canterlot he cuts the wires of the controls while declaring:"The days of chivarly do not end by your hand!"
- Honor Before Reason: While he doesn't trust Limburger ( and he is even warned by Throttle ), he is forced to accept his challenge to a duel by the rules of chivarly.
- I Gave My Word: He gives Limburger control over Camelot after his knights are defeated, just like they agreed. Thankfully the Biker Mice stepped in.
- Obnoxious In-Laws: He decided to name the root beer Modo creates like his queen's mother, hoping it will make " that dragon " shut up.
- Reasonable Authority Figure: He knew from the start that Limburger was a bad guy and was also fair to the Biker Mice and willing to let them prove they were good guys.
- Royals Who Actually Do Something: He personally leads the assault to Limburger's fortress.
King Arthur's faithful wizard companion.
Tropes associated with Merlin:
- Big Damn Heroes: He arrives just when the Biker Mice are without fuel and about to get shot by Greasepit and Karbunkle. First he deals with the two, then he reveals he was able to create gasoline in his lab.
- Cool Old Guy: He successfully finds a way to magically refuel the Biker Mice's bikes when they run out of gasoline and also gets a chance to pay back Karbunkle for beating him.
- Magic Versus Science: His duels against Karbunkle. He was initially defeated, but evened things out in the second fights.
- Miniature Senior Citizens: He is much shorter than King Arthur.
- Pretender Diss: In their second duel he calls Karbunkle a pretend wizard and hands him his ass.
- Teleporters and Transporters: He has shown this ability at least twice.
- Wizard Classic: Looks exactly the way wizards are usually depicted in fiction, pointy hat and long white beard included.
"Asphalt" Jack McCyber
An old friend of Charley's who is also technologically skilled enough to create a virtual reality helmet.
Tropes associated with "Asphalt" Jack McCyber:
- Amicable Exes: It is implied that he and Charley used to date. While they're no longer together, they're still shown to get along well.
- Chuck Cunningham Syndrome: Another character who doesn't show up in the revival, which is particularly noticeable because of his history with the Biker Mice's most prominent human ally Charley.
- Cool Bike: What he has been working on.
- Gadgeteer Genius: He's a skilled inventor and computer expert.
- Jerkass: It was probably unintentional, but he doesn't give a good first impression: he sneaks in Charley' s garage when nobody is home, immediatly draws a gun to the Biker Mice (without even asking who they are or what they want), do the same to Charley until she recognizes him and, when Vinnie makes a joke about his manners, he treatens to punch him in the mouth.
- Interspecies Romance: Not that he had any idea he was shocked and disgusted when he discovered his fiancè Angela was a Plutarkian
- Meaningful Name: He's technologically inclined and his surname is McCyber.
The Martian Freedom Fighters
Stoker is the creator and main force behind the might of the Freedom Fighters. He is a legend amongst all Martians, and maybe even beyond that planet itself. However, age and a hard lifestyle forced him to slow down, although he resisted toning down his signature style of tail-whipping for a while. He is Throttle's mentor, Vinnie's idol (and occasional foil) and a dear friend to many Freedom Fighters.
In the 2006 revival, he attempts to retrieve his invention the Regenerator from Ronaldo Rump and the Catatonians so that he can build a new one and use it to end the drought on Mars.
Tropes associated with Stoker:
- Ascended Extra: He only appeared a handful of times in the original series, but is a major character in the revival.
- Bad Ass Biker: He was the best biker on Mars (and in the Whole Galaxy, according to him), before old age caught up with him.
- Brainwashed: In the flashbacks taking place on Mars in "Once Upon a Time on Mars", Karbunkle hypnotizes him into serving the Plutarkians, but he ends up coming to his senses.
- Cool Old Guy: He is implied to be older than Vinnie, Modo, and Throttle. In fact, by "Caveat Mentor" he became too old to actively fight, even if he desperately tried to prove otherwise.
- Dark Is Not Evil: In the 2006 series, he is Nightshift, a biker dressed in black and with red eyes in his helmet, but is one of the good guys.
- Darth Vader Clone: His Nightshift bears some vader resemblence and the first episode gives him a dramatic reveal.
- Jekyll & Hyde/Hulking Out: In the 2006 series, exposure to sunlight causes him to transform into a rat-like monster.
- The Kirk: Balances the differing personalities of Rimfire and Carbine.
- I Want My Beloved to Be Happy: Holded absolutely no hard feelings toward Harley for choosing Vinnie.
- La Résistance: He was the founder and the leader of the Fredoom Fighters, the only true force opposing the Plutarkians during the invasion of Mars.
- The Leader: Until old age forced him to step down, passing the torch to Carbine.
- Love Triangle: He was in one on Mars, where both he and Vinnie liked Harley. He lost
- Long-Lost Relative: The 2006 series reveals that he has a daughter named Spitfire, who was separated from him between the events of the 1993 series and the revival.
- Mentor Archetype: According to Modo, he is the one who trained the Biker Mice. When his age made him lose his fighting skills, Charley suggested he can still do this to the younger Fredoom Fighters like Rimfire.
- Mind-Control Eyes: His eyes glow green when under the control of Karbunkle's mindbender beam in "Once Upon a Time on Mars".
- Papa Wolf: In the 2006 series episode "Once Upon a Time on Earth", his rat form attacks Cataclysm when he sees him threaten his daughter Spitfire.
- Red Eyes, Take Warning: Subverted when he is Nightshift, but played straight when he turns into a monster in the 2006 series.
Rimfire is a sprightly, athletic young mouse—as said above, Modo's sister's child. Along with his sister Primer, Rimfire has fought in the Mars-Plutark war despite being only around 16 or 17 years old in human years. Like Modo, Rimfire is polite and gentle, as well as having a ninja-like talent for getting out of tight scrapes (and into tight scrapes: only one episode has him not being held captive by a Big Bad).
Tropes associated with Rimfire:
- Demoted to Extra: He was a semi-recurring character in the original series, but is only present in one episode in the 2006 revival.
- Distressed Dude: Has been held captive quite a few times.
- The Klutz: For some reason he's become unbelievably clumsy in the 2006 series.
- Hero-Worshipper: A downplayed example, but he greatly admires his uncle Modo and couldn't wait to became a freedom fighters like him.
- La Résistance: Is a member of the freedom fighters.
- The McCoy: He lets his love for his uncle Modo cloud his rationality.
- She-Fu: A Rare Male Example: his fighting style includes lots of cartwheel and backflips before he hits his opponents.
- Skunk Stripe: He has an orange stripe on brown hair.
- Wimpification: For some reason the same Rimfire who was shown to be a good biker and fighter in the original series is reduced to an inexpert, naive klutz in the 2006 sequel.
- You Don't Look Like You: He has a different hairstyle and is colored brown in the 2006 series.
Carbine is the new leader of the Freedom Fighters, a former Army General. Unlike Stoker, she prefers a set of rules and disciplines, instead of the camraderie and mild chaos of Stoker's FF. Despite this, she is still a very competent leader, although her desire to stay on Mars has driven a wedge between her and her boyfriend Throttle. She nearly had him executed after he returned to Mars just after the Plutarkians seeded a tape of him and his bros supposedly aiding them in destroying Chicago, but they managed to hash it out before the Biker Mice were thrown into the Pit of Everlasting Doom (otherwise known as Spiky Pit of Spikes). She may come as a bit touchy at times, but it's only because she cares most about her planet's survival.
In the 2006 revival, she is revealed to be Stoker's niece.
Tropes associated with Carbine:
- Alliterative Name: Although her surname is rarely used.
- Action Girl: Accomplishes quite a lot of victories as a member of the freedom fighters.
- Ascended Extra: She only appears in a few episodes of the original series, but has a more important role in the 2006 revival.
- Badass Longcoat: In the 2006 series, she wears a long red coat.
- Good Scars, Evil Scars: She got a scar on her face shortly after the Biker Mice originally left Mars, but she is still on their side for the most part.
- Jerk with a Heart of Gold: She made it difficult for Throttle and the others to clear their names when she was convinced that they helped Limburger, but she and Throttle ended up patching things up and parted on better terms by the end of the episode featuring her. She also accepts that the Biker Mice like staying on Earth in the 2006 series.
- Mission Control: In the 2006 series, she gives Throttle, Vinnie, and Modo the orders they need to follow in their mission to find Stoker, retrieve the regenerator, and defeat the Catatonians.
- La Résistance: As noted, she runs it.
- Rugged Scar: She has a scar to show that she suffered while trying to survive the war against the Plutarkians.
- The Spock: The most logical of the freedom fighters.
A Martian mouse who fought against the Plutarkian invasion of Mars.
Tropes associated with Sergeant Scabbard:
- Alliterative Name: His name and title both begin with S.
- Chuck Cunningham Syndrome: He's the only Martian mouse allied with the Biker Mice to not appear or be acknowledged in any capacity in the 2006 series.
- Last Episode, New Character: He isn't introduced until the three-part episode "Once Upon a Time on Mars", the finale of the 1993 series.
- To Be Lawful or Good: He has some conflict over following the orders given to the Martian military or doing what he knows is right. Carbine manages to bring him over to the latter.
Vinnie's old girlfriend on Mars. She was the one who patched up his face by giving him the metal plate he wears. In the finale of the 2006 revival series, it turns out that she ended up disfigured while trying to evade the Plutarkian soldiers and sided with the Rats because she believed the Biker Mice abandoned her.
Tropes associated with Harley:
- Back for the Finale: In the original 1993 series, she only appeared in the three-part finale "Once Upon a Time on Mars". The 2006 revival has her return in the final episode "Turf Wars".
- FaceHeel Turn: In the 2006 series, it is revealed that she sided with the Nomad Rats when she believed that the Biker Mice abandoned her.
- Heel Realization: While she intended to use the regenerator to make Olympus Mons erupt in "Turf Wars", she eventually sees the error of her ways after realizing how devastating that course of action really is.
- Heroic Sacrifice: When the regenerator was in danger of going critical, she risked her life to get close enough to turn the device off and shortly after that was supposedly killed by some lava.
- Last Episode, New Character: In the original 1993 series, she wasn't introduced until the three-part episode "Once Upon a Time on Mars", the finale.
- Never Found the Body: Even though she is supposedly killed after she stops the regenerator from going critical, her body is never seen and Charley and Modo even try to cheer up Vinnie by telling him it's possible she survived somehow.
- Please Keep Your Hat On: In the 2006 series, she wears a purple mask. The flashbacks that play during her Villain Song reveal that the mask is to hide the scars she sustained during the Plutarkian invasion.
- Redemption Equals Death: She eventually comes to her senses and rejoins the Biker Mice, but she supposedly sacrifices her life to prevent her plan from causing irreparable damage to the already drought-ridden Mars.
- Tragic Villain: She was once Vinnie's love interest, was disfigured while trying to flee from the Plutarkians, she sided with the Rats because she was convinced that the Biker Mice gave up on her, and she supposedly dies after she sets things right. Sounds about right.
- Villain Song: "Hard to be Harley" in the 2006 series episode "Turf Wars", where she sings about what happened to her after Mace kidnapped her and why she's betrayed the Biker Mice.
A yeti encountered by the Biker Mice in "A Hairy A-Bomb".
Tropes associated with A-Bomb:
- Bigfoot, Sasquatch and Yeti: He is a yeti.
- Learnt English from Watching Television: He mentions that his kind learned English by observing humans' broadcasts.
- Manly Tears: Sheds tears when the Biker Mice allow him to use the cluster of tetrahydrocarbons to help his own people when it could have been used to help Mars.
- Only Known by Their Nickname: A-Bomb is merely a name given to him by Charley. His actual name, if he even has one, is unknown.
- Running Gag: He constantly has trouble pronouncing "abominable snowman".
A Catatonian on Saturn's moon Titan who defected from his race alongside several other peaceful Catatonians.
Tropes associated with Tane:
- My Species Doth Protest Too Much: He and the other Catatonian surfers on Saturn defected from their race because they weren't pleased by their evil ways.
- Surfer Dude: He has the speech patterns and mannerisms of a surfer dude.
A race of alien rabbits who live on the moon due to being exiled from Earth. The Catatonians made a deal with them that they'd return them to Earth in exchange for their services, but reneged on their promise.
Tropes associated with Tudezian Rabbits:
- Alliterative Name: Razer Rabbit's first name and last name both start with R.
- The Exile: It's established that they used to reside on Earth, but were forced to leave when the humans grew tired of their tricks.
- Gadgeteer Genius: They are skilled with machinery.
- Insistent Terminology: Razer insists that his people be called "rabbits", not "bunnies".
- Nice Hat: Razer wears a cap.
- No Name Given: Pops Rabbit and Razer Rabbit are the only ones named.
- Rascally Rabbit: They're all tricksters at heart.
- Righteous Rabbit: They're alien rabbits who end up working with the Biker Mice and helping them outsmart the Catatonians.
- Species Surname: Pops Rabbit and Razer Rabbit are both alien rabbits with Rabbit as their surname.
- Stock Animal Diet: As is often the case in fiction, these rabbits like to eat carrots.
- The Voiceless: Pops Rabbit and Razer Rabbit are the only ones who have any dialogue.
Tropes associated with Spitfire:
- Long-Lost Relative: She was separated from her father some time between the events of the 1993 series and the 2006 series and doesn't reunite with him until the conclusion of the three-part episode "Once Upon a Time on Earth".
- Missing Mom: Absolutely no mention is made of her mother.
- Wham Line: Her last line of dialogue in part two of the three-part episode "Once Upon a Time on Earth" changes everything.Spitfire: Because I'm the package Stoker wanted. I'm his daughter.