A list of DC Animated Universe characters who first became prominent in Batman: The Animated Series.
- Batman and Bat Familynote Nightwing, Batgirl, Robin, Alfred Pennyworth
- Alliesnote James Gordon, Harvey Bullock, Renee Montoya, Zatanna, Jonah Hex, Hamilton Hill, Lucius Fox, Summer Gleeson, Creeper, Leslie Tompkins, Veronica Vreeland, Gray Ghost, Yoru Sensei, Kairi Tanaga, Charlie Collins, Batwoman, Sarge Steel, Swamp Thing, Etrigan
- Rogues Gallery Part 1note Penguin, Two-Face, Poison Ivy, Catwoman, Ra's Al Ghul, Talia Al Ghul
- Rogues Gallery Part 2note Mister Freeze, Riddler, Scarecrow, Mad Hatter, Clayface, Man-Bat, Killer Croc, Ventriloquist & Scarface, Bane, Clock King, Baby Doll, Firefly, Phantasm, Roxy Rocket, Floronic Man
- Rogues Gallery Part 3note Rupert Thorne, Roland Daggett, Doctor Milo, Hugo Strange, Red Claw, Kyodai Ken, H.A.R.D.A.C., Mojo, Arkady Duvall, Gregory Belson, Grant Walker, Ferris Boyle, Lock-Up, Anthony Romulus, Vertigo, Maxie Zeus, Calendar Girl, Mad Bomber, Josiah Wormwood, Sidney Debris, Salvatore Valestra, Sewer King, Arnold Stromwell, Condiment King, Tony Zucco, The Terrible Trio, Boss Biggis, Jazzman, Vinnie the Shark, Captain Clown, Chuckie Sol, King Barlowe, Carlton Duquesne, Lady Shiva, J. Carroll Corcoran, Boxy Bennett, Gil Mason, Arthur Reeves
- Rogues Gallery Part 4: Minions
