Otis is the leader of the barnyard. He is a cow.
- Animal Gender-Bender: He's a male cow with an udder. There's something not right there.
- Belligerent Sexual Tension: With Abby.
- Bunny-Ears Lawyer: More pronounced in the series, where he's regularly a goofy idiot but comes up with quality plans when necessary.
- Butt-Monkey: Is prone to a ton of slapstick. Though, he brings it upon himself most of the time.
- Catch Phrase: "Milk me!"
- Flanderization: The movie has his personality rather different from the series. In the movie, he's fun loving, but still knows how to think up good ideas. However, in the series, Otis is more goofy and incompetent about this "heroism" business.
- Genius Ditz: As foolish as he can be, he has a lot of clever ideas on how to fix the mistakes he's made.
- Idiot Hero: He doesn't know a thing about leading, but is a hero nonetheless.
- Insult Misfire: Does this often
- It's All About Me: His reason for interrupting a broadcast instead of just stealing the tape of them talking?Otis: Cause that dont put Otis on television.
- Jerkass Ball: Some episodes will involve him into this. "Save the Clams" and the Halloween specials remain a couple of the more prominent examples.
- Jerk with a Heart of Gold: At his worst, he can be quite insensitive and selfish, but he genuinely cares about his teammates.
- Mr. Exposition: Does this often in the series. With Pig has calling him both "Professor Exposition" and "Captain Exposition" on two different occasions.
- Oblivious to Love: Otis is quite unaware of Abby's very obvious crush on him. He won't admit his feelings for her either.
- Took a Level in Dumbass: In the series.
- Unsympathetic Comedy Protagonist: At his worst.
Otis' best friend. A mouse.
- Deadpan Snarker: Self proclaimed, not all that justified.
- Failure Is the Only Option: Whenever he hits on Bessie.'
- Has a Type: In one episode, he admits that he's into women who are bigger than he is.
- Hidden Depths: One episode shows that he can be a quality leader under most circumstances.
- Hot Skitty-on-Wailord Action: His crush on Bessie.
- The Lancer: In comparison to Otis
- Precocious Crush: His crush on Bessie. Okay, he's not exactly a kid, but he's a mouse. Enough said.
- Unaffected by Spice: In "The Good, the Bad and the Snotty", Snotty Boy feeds the animals pizzas laced with hot sauce. While everyone has the standard reaction, Pip is able to stomach it and calls the others lightweights.
- Yank the Dog's Chain: In the episode where Bessie thinks she's Otis' mom, she believes that she needs somebody else to help her handle her kid. She comes so close to marrying Pip before Otis finds a newspaper article that proves he and Bessie are not related.
A female cow at the barn who has a crush on Otis and vice versa, though they never get to admit their feelings for each other.
- Action Girl: For one thing, she's an excellent wrestler.
- Belligerent Sexual Tension: With Otis.
- Betty and Veronica: "Abby & Veronica" plays with it. Veronica is Abby's old friend that actively charms Otis and the other guys into doing whatever she wants. Veronica doesn't love any of them, but it nonetheless makes Abby jealous and angry, so she tries harder to woo Otis.
- Expy: Of Daisy from Barnyard, appearance-wise.
- Girly Bruiser: Very dainty and feminine, but don't let that fool you into thinking she can't fight.
- One of the Boys: Which she doesn't mind until her old friend/rival Veronica visits and all the guys fawn all over her.
- Raised by Dudes: She mentioned before that she was the only girl in a family full of brothers.
- The Smurfette Principle: Downplayed. Bessy is a major character, but Abby appears far more often and is more likely to take part in shenanigans with Otis and the others.
- Southern Belle: She talks like one.
- Super OCD: One of her many weaknesses.
- Suspiciously Similar Substitute: To Daisy from The Movie.
- Tomboy with a Girly Streak: Loves masculine hobbies such as wrestling, but also enjoys using stuff like make up every now and then.
A pig who lives at the farm and hangs out with the main characters.
- Big Eater: He once ate a lifetime supply of Flaky Tarts (basically Pillsbury toaster strudels) in one afternoon.
- Breaking the Fourth Wall: He often interrupts the show just so that he can answer fan mail.Pig: (reading letter) Dear Pig, aren't you interrupting the story at the most suspenseful part? Why, yes Sally. Yes, I am.
- Breakout Character: A relatively minor side character in the movie, but in the series has a majority of cutaway gags and his own intermissions.
- Cloudcuckoolander: Not to Freddy's extent, but he can be quite foolish including believing in leprechauns or unicorns.
- The Cuckoolander Was Right: One episode had him say that maybe a leprechaun stole the Farmer's potatoes. He was right!
- A Dog Named "Dog": He's the only barn animal named after its species.
- Odd Name Out: For the most part, as the other characters (besides Peck) tend to have human names or names that could pass for such.
A ferret who lives at the barn. Best friends with Peck the rooster, and constantly scold himself for thinking about eating him or any other chicken on the barn.
- Adorkable: His shy and neurotic nature just makes it hard not to love him.
- Amazing Technicolor Wildlife: Freddy has orange fur, which isn't something you see on real ferrets.
- Art Evolution: He becomes noticeably less hairy than he was in The Movie.
- Break the Cutie: "Meet The Ferrets", "Dummy And Dummier", and "Fowl Play" really push him into woobie territory.
- Butt-Monkey: Probably not to Peck's extent, but he tends to suffer a lot of abuse, too.
- Crippling Overspecialization: In one episode Freddy was shown to be talented at woodcarving, however the only thing that he knew how to make with the skill is Cups meant to hold Pencils. Even when Otis is in danger and they need to create a Trojan Horse to get him out of trouble and they need Freddy to make it, he simply spends hours carving a gigantic Pencil Cup, and criticizes the others for creating an actual Trojan Horse by complaining where the pencils are supposed to go inside of it.
- Crouching Moron, Hidden Badass: Despite his dim-witted nature, he's managed to save his friends from his ventriloquist puppet, Mr. Jinx (which was controlled by ants as revenge for them unknowingly destroying their home).
- Cloudcuckoolander: Has a lot of rather abnormal traits to him.
- The Ditz: Is easily the more dim witted of the farm animals next to Pig and, to an extent, Otis.
- Dumb Blonde: Blonde skin + ditzy nature = this trope.
- Hair of Gold, Heart of Gold: Played With. While he has a tendency to crave Peck, he's mostly well-meaning in his own way. His golden fur certainly helps him fit this trope.
- Horror Hunger: He must discipline himself not to eat any barn animals, especially not Peck.
- Informed Species: He doesn't really look like a ferret. Instead, he looks more like a skinny hyena.
- Keet: When he isn't trying to eat Peck.
- Odd Friendship: With Peck, a guy he has tried to eat more than once.
- Plucky Comic Relief: Tries to be, though he's more of a Butt-Monkey.
- Sad Clown: He has his moments. Also, considering the fact that he has all sorts of psychological problems.
- Took a Level in Dumbass: In the series, to the point where even Otis has to ask how dumb he is in "Beady and the Beasts" after sniffing an exploding flower the group just planted.
A rooster who lives at the barn. He's best friends with Freddy the Ferret.
- Butt-Monkey: More so than Freddie
- The Chew Toy: He gets most of the accidents out of all the animals. A parody of an On the Next segment was made up exclusively of clips of Peck being injured in different ways.
- Odd Friendship: With Freddie, a guy who has tried to eat him more than once.
- Only Sane Man: Very possibly. He might share this with Pip.
A cow who lives on the farm and criticizes Otis.
- Dark and Troubled Past: According to one episode, she had a son who went off to chase balloons at a fair and hasn't seen him since.
- Deadpan Snarker: More often than not
- Flanderization: In the movie, she was slightly aloof but, nevertheless, sociable, however, the series plays up the aloofness.
- Jerk with a Heart of Gold: She is close friends with Abby, and she does actually care about the other animals.
- Sassy Black Woman: Her voice actor plays her this way. She's also a brown cow.
- Took a Level in Jerkass: She's much more shrill than she was in the movie. She even seemed okay with Otis being sold to a steakhouse!
- Grumpy Bear: Tends to have a rather sour mood.
- I Owe You My Life: "Cow's Best Friend" follows the basic beats, with Duke annoying Otis to no end and not stopping until the score is even. Otis fakes various situations, has to repeatedly save Duke whenever things go wrong, tells the truth, gets saved for real, and is told he owes Duke for the various incidents that endangered his life.
- Only Sane Man: Often expresses mundane and cautious courses of action.
A human who lives close to the farmer. She knows that the animals walk and talk, and is always looking for evidence, causing her husband to believe she's crazy.
- Ascended Extra: Originally more of a joke than anything in the original movie, she appears much more frequently in the show, making much more of an effort to expose the animals. Doubles as From Nobody to Nightmare with how antagonistic she's gotten towards the animals since those days.
- Aw, Look! They Really Do Love Each Other: In Beady and the Beasts, it's shown that as crazy as he thinks she is, Nathan still loves Nora. It's telling that after she comes back from being kicked out by Nathan's overbearing mother, he doesn't hesitate to stand up and try to hug her.Nathan's Mother: Fine, I know when I'm not wanted.Nathan: You're just less wanted. Bye, ma!
- The Cassandra: When she tries to point out what the animals are doing, she's never believed.
- Chronic Villainy: To the animals, anyway
- Deadpan Snarker: This is subtle
- Housewife: Even if her husband does think she's lost it.
- Ineffectual Sympathetic Villain: Episodes such as "Saving Mrs. Beady" will make you feel more sympathy for her than any other episode.
- Last-Name Basis: As revealed in the final scene of the movie, her last first name is "Nora".
- Mama Bear: Mess with her nephew, and you'll suffer the consequences.
Mrs. Beady's nephew who likes to mess with the barn animals.
- Fat Bastard: He's a Jerkass, who just so happens to be on the pudgier side
- Fat Idiot: He's not terrifically bright.
- Jerkass: This is the reason as to why he's called "Snotty Boy"
- Knight of Cerebus: Snotty Boy is usually more of an unbearable annoyance than a threat. But then came Snotty's New Pet, where he captures Pip and tried to feed him to his snake, even after the snake refused. While he ain't no Dag, this might be the only time he came close to ending one of the main characters. The way he treats that poor snake makes it even worse; not only does he abuse him, but the affects said abuse has on the snake are eerily reminiscent of those of real life abuse victims.Pip: Why do you let him do that stuff to you?
Snake: Nah, I deserved it because I disrespected him.
- Harmless Villain: More of a bother than a threat to Otis and friends.
- Meaningful Name: He's a snotty boy
- Only Known by Their Nickname: His real name is Eugene, as mentioned in a few episodes
Two guys who work at a pizza restaurant and pop up to say stupid things.
- Catch Phrase: "Doodley Doodley Doo Doo!"
- Those Two Guys: They're always together.
- Took a Level in Dumbass: From the movie, they hardly appeared, even though they were pretty stupid. In the series, they're recurring characters, and are equally, if not more stupid.
The owner of the farm. He's not a very important character, and he's unaware of the animals' ability to walk and talk.
- Butt-Monkey: Less so than Peck or Freddie and this was mostly in the movie and earlier episodes.
- Everyone Calls Him "Barkeep": To the point where the animals are surprised to learn what his last name is.
- Hero of Another Story: Several episodes allude to him having a very wide variety of hobbies and interests that he tends to be absent from the barnyard for. We never really get to see them beyond cutaway gags.
- No Name Given: We don't know what his full name is, though one episode reveals that his last name is Buyer (which leads to a hilarious misunderstanding on the animals' part).
- Out of Focus: He appears more in the earlier episodes. In later episodes, he tends to be off doing other stuff and appears more infrequently.
- Took a Level in Dumbass: In the film, he didn't really appear much. In the series, he appears more, but possesses more stupidity than he would have in the movie.
The local Veterinarian who's feared by the animals she treats.
- Conspicuous Gloves: The Vet seems to never take off her pair of latex gloves, to the point that it's implied her name is actually "Dr. Glove" by Pip in "Dead Cow Walking". However, given her hands-on approach to medicine, it's not surprising she feels the need to constantly glove up.
- Glove Snap: In the episode "Dead Cow Walking" the Vet has tendency of doing this whenever she performs a "checkie-poo". Ugh, let's just hope she changes gloves afterwards.
- Morally Ambiguous Doctorate: The procedures the Vet prescribes for her patients are often ungodly cruel (two words: butt leeching).
- Obliviously Evil: The Vet cares deeply for all the animals she treats. Despite this, she often puts them through a great deal of pain.
- You Don't Look Like You: The Vet is given two redesigns in the series: one in " Snotty's New Pet" and another in "Doggleganger". Though it may be implied that these redesigns may just be different characters with similar attire, personalities, and voice actresses, it's not made entirely clear.
- Voiced by: Grey Delisle
A horse riding rodeo performer who The Farmer becomes smitten with.
The Jersey Cows
Three trouble-making cows.
- Only Known by Their Nickname: They are only referred to as The Jersey Cows. Their names being revealed in the credits.
- Took a Level in Jerkass: As mischievous as they were in the movie, they were still Otis's friends and had his back when things got tough. Now they're complete jerks in their appearance in the series.
- Voiced by: Rob Paulsen
A bizarre creature covered almost entirely in hair.
- A Day in the Limelight: "Wild Mike's Dance Party".
- Ax-Crazy: True to his name, Wild Mike is absolutely insane.
- Cartoon Creature: It is unknown exactly what kind of animal he is, although some speculate him to be a monkey due to his humanoid arms and legs.
- The Faceless: Played with, his face is covered in a huge mess of hair, although his eyes can occasionally be seen upon closer inspection.
- Laughing Mad: His main way of communicating, besides unintelligible screaming, is through insane laughter.
- The Unintelligible: He only communicates through psychotic screaming and laughing.
- Demoted to Extra: While he hardly served as anything more than a Joke Character in the movie, he only makes one appearance in an episode of the TV series, aptly titled "Wild Mike's Dance Party".
- Voiced by: Sam Elliott
A cow and Otis's adoptive father, as well as the leader of the Barnyard animals.
- Arc Words: "A strong man stands up for himself. A stronger man, stands up for others."
- Badass Adorable: A cow who defends the other barn animals from harm. He also has a playful side when he is teasing with Otis.
- Badass Baritone: With the voice of Sam Elliott, this is a given.
- Beware the Nice Ones: He's amiable and pleasant, but don't be fooled into thinking that he's a pushover because of this. The coyotes learned it the hard way.
- Famous Last Words: "Put the hen down, Dag."
- Grievous Harm with a Body: During the fight with the coyotes, he grabs Dag and uses him as an impromptu weapon.
- Heroic Sacrifice: Dies protecting the hens from the coyotes.
- Jerk with a Heart of Gold: Although stern, he does show genuine love for Otis and does care for the barnyard animals' safety.
- Killed Off for Real: The first Nickelodeon character to die; more the first one we actually have a death scene for.
- Nice Guy: All-round friendly and pleasant with others, even Otis, despite the fact that they butt heads often. He makes it exceedingly clear that he cares about Otis' well-being, even though the latter realizes it too late.
- Not Worth Killing: He basically won his fight against the coyotes - albeit at the cost of his own life - but upon seeing what a Dirty Coward Dag is, Ben just lets him run away instead of finishing him off.
- One-Man Army: It takes six coyotes working together to kill him, and he still forces them to retreat before succumbing to his injuries.
- The Patriarch: Fittingly for a middle-aged black cow, he's the tough guy of his family. He protects the other animals from harm.
- Rated M for Manly: He is a pretty tough and manly cow. Despite having an udder.
- Voiced by: Courteney Cox
A pregnant cow who arrives on the farm with Bessy who becomes Otis' love interest.
- Chuck Cunningham Syndrome: Neither she nor her child are seen or even mentioned in the series. Especially odd since she was Otis's Love Interest in the movie.
- Flat Character: She's a pretty bland love interest. Her being sweet and nice is pretty much her only characteristics. She is mainly used as a device to help Otis become a man.
- Somewhat subverted as the creators do try to give her a Tragic Backstory but it could be cut out of the movie entirely.
- Nice Girl: Immediately takes a liking to Otis.
- Sole Survivor: Daisy and Bessy are the sole survivors of a storm that wiped out their herd.
- Voiced by: Danny Glover
An elderly mule and longtime friend of Ben.
- Cool Old Guy: Maintains peace in the barnyard. He also had nothing but respect for Ben and became a father figure for Otis after his death.
- Nice Guy: He is very kind-hearted, helpful, and wise.
- Voiced by: David Koechner
A cruel and carnivorous coyote and the leader of a pack of coyotes that frequently terrorize the farm.
- Ax-Crazy: He's a psychopathic "lady killer" in his own words. Not only does he keep parts of the corpses of his prey to use as trophies, he clearly gets a kick out of killing other animals. It's subtly implied that not even humans are safe from him, as he tells Otis that, if he breaks their deal, he and his pack will slaughter everyone in the farm, the farmer being presumably included.
- Big Bad: The leader of the coyote pack that is terrorizing the farm. Naturally, he is also the most cunning and dangerous of them all.
- Dirty Coward: Dag prefers to sic his pack on others and waits for an opportunity to sneak attack others when they don't see him coming. He also begs and cowers for his life the moment things go south for him.
- Establishing Character Moment: The very first thing he does in the movie is sneaking into the chicken coop with his pack and terrorizing the hens with his chain, which is decorated with the legs of his previous victims, all while sadistically chuckling. All of it demonstrates just how murderous and out of his mind he really is.
- Evil Laugh: A snorting chuckle which only makes him more creepier.
- Evil Sounds Raspy: His voice is gravelly and hoarsy.
- Expy: He's quite similar to Wile E.Coyote just more cunning and of course more sinister and evil.
- Faux Affably Evil: Just listen to how Dag talks to the chickens as he is about to take and eat the chickens. One example is his first scene:Dag: Good evening ladies. Sorry to call you so late in the evening but we did have a previous engagement. (That's when he gets out chicken feet chain to scare the hens with)
- Hate Sink: Dag is utterly detestable in every conceivable way.
- Jerkass: Aside for being a vicious predator with a taste for killing, he's also a bully who loves to taunt and insult his victims. This is shown when he makes fun of Otis about his weight.
- Kick the Dog: If killing Ben and mocking Otis about it wasn't bad enough, he also tries to eat Maddy just because she called him a "meaner".
- Knight of Cerebus: A murderous coyote who sucks every last bit of lightheartedness in the movie whenever he's present. Him and his pack kill Ben, which forces the fun-loving Otis to step up as new leader of the farm animals.
- Law of Chromatic Superiority: Has red-tinted fur and is the leader of his pack.
- Lean and Mean: His ribcage is always in good view despite presumably eating many chickens.
- Sadist: While one could make the argument that he's only killing for food, the fact that he gets enjoyment out of murdering and eating hens, even keeping their severed feet on a chain, shows that he's ultimately this.
- Serial Killer: A sadistic murderer in coyote's skin, as evidenced by his chain of chicken feet.
- The Sociopath: To be his most ruthless and evil nature.
- Vile Villain, Saccharine Show: A goofy animated movie about partying farm animals, with a sadistic coyote who shows signs of being a serial killer. Why not?
- Villain Has a Point: Though he delivers it in a colossal Kick the Dog way, he did make a valid point in saying that Ben would probably still be alive had Otis decided not to flake on his duties of guarding the barnyard on the night when Dag and his pack snuck onto the farm and killed Ben.
- Would Hit a Girl: He wants to eat the chickens and the chick Maddy.
- Would Hurt a Child: He certainly wasn't going to spare Maddy just because of her young age.
- Voiced by: Andie MacDowell
A mother hen.
- Demoted to Extra: She only had two speaking lines in the series, and maybe a few more nonspeaking cameos, but that's it.
- Mama Bear: As shown before, picking on Maddy will be a regrettable action
- Voiced by: Madeline Lovejoy
A young chick who enjoys playing with Otis. She is slightly troublesome for her mother Etta to take care of because she often wanders off from the chicks.
- Badass Adorable: She is as cute as a button and stood up to Dag calling him a "meaner"
- Big Brother Worship: She certainly seems to view Otis as a heroic big brother, and the later definitely doesn't mind. It's when Dag kidnaps her and the rest of the hens that he finally decides he's had enough of the coyote.
- Children Are Innocent: Looks up to Otis and hates Dag.
- Nice Girl: She is quite compassionate. She immediately tries to comfort Otis when she sees him mourning for his father.
- Ridiculously Cute Critter: Has a large head and small body.