In the first installment of The Gamers, Rogar does one in a flashback to a previous session, at "the battle of Black Fields, where Rogar's blood brother was killed."
Smosh did this a lot during their classic era (2006-17). Some notable examples include the videos "VADER AND ME!" (when Anthony reacts to Ian telling him that Vader was his father, although he was only joking), "F*CK SPOILERS!" (yelled by a black man when Ian and Anthony inform him he is indeed black, but he quickly changes his tune when he realizes it means he has a large penis), "JURASSIC POKÉMON" (yelled by Oak when he's told the power to his sauna has gone out), "MY MORNING ROUTINE" (when Anthony is forced to ingest beans and Ex-Lax), "MY NEW HOT GIRLFRIEND" (yelled by Ian's inner monologue as he witnesses Erin and Anthony making out), "REAL MARIO LAVA FLOOR!" (yelled by Anthony as Ian is about to kill himself via lava floor), "MAGIC IPOD" (yelled by the iGenie when he finds out Avril Lavigne married Chad Kroeger of Nickelback), twice in "Super Powers!" (yelled by Ian when Anthony suggests watching the Speed Racer movie, and by Anthony when Ian drinks his glass of water) and three times in "WORST PROPOSALS EVER!" (yelled by Ian when Antoinette dumps him, by Anthony when he finds out his mom is a man, and by Antoinette when she changes her Facebook relationship status to "single" and no one likes it)
The Food Battle videos had a brief running gag where Ian would deny the Food Battle Announcer Guy celebrating his victory with him, in "Food Battle 2006" and "Food Battle 2008". In "Food Battle 2009", he lets him watch paint dry with him, but screams a big "NO!" at him after he asks repeatedly if the paint is dry. In "Food Battle 2010", the Announcer Guy screams "NO!" when Anthony asks him if he wants to play with Pogs. Anthony screams "NO!" in "FOOD BATTLE 2011" when the Announcer Guy dies before proclaiming him the winner of Food Battle. It was also yelled out by the main characters in four Food Battle campaign videos (Egg Roll and Mozzarella Stick in 2012; Candy Cane and Chocolate Bar Donut in 2013).
This Photoshop comic parodying A&W's commercials shows Ryan throwing Allen a birthday party. Allen invokes this trope when Ryan announces he's giving away free Mama Burgers.
Season 2, Episode 1 of Jon Bois's web series "Pretty Good", aptly titled "NO!!!!!!", features and discusses one of the most famous moments of this in sports history: The River City Relay, and John Carney's missed extra point attempt to keep the Saints in the Playoff hunt in 2003. At one point, forty seconds of video are spent just repeating color commentator Hokie Gajan's horrified "NO!" in increasingly distorted, nightmarish ways.
Necro Critic did this when he found out there was more to Ehrgeiz than just the terrible fighting game. This one lasted past his 30-second long end screen and continued even after stopping for air.
Movie Rehab: At the end of his Southland Tales review where Sag tries to deal with Trama to kill him instead of Rumpy. Trama then tells him that he's going to kill Rumpy anyway which then causes Sag to yell those exact words. Rumpy does get saved in the last second though.
In Episode 8 of Battle For BFDI, Bubble does this when she gets demoted to Bemb.
Loser lets out one in his head in Episode 17 when a Fan is doubting him.
A popular internet meme is RAEG, where a template comic strip is drawn on, with the ending of a grotesque face shouting "FFFFUUUU—" in situations like a no smoking sign, being caught watching porn, etc.
The Leet World's Chet usually greets the death of his drinking buddy Player with a cry of "Player, NOOOOO!" However, since the show is set inside a Video Game, dead characters are usually back in action next round. A notable exception is when Player is injured by Ahmad's grenade, as this took place outside the challenge levels where death is permanent. Lampshaded in the episode "Thunderhead" when Player breaks cover runs into the line of fire. Westheimer shouts "Player, No!" and Chet comments that it sounds better when he says it.
In the Zero Punctuation review of Spore, Yahtzee delivers a deadpan Big No. Twice. Both times in his inimitably snarky manner.
"So can his new game Spore possibly live up to that legacy? In short, no. In long, noooooooooooooo..."(total length: 17 seconds)"...ooo. So I guess what I'm asking is, 'Can brightly coloured monstrosities really compare to Batman?' Short answer, Nooooooooo..." (interrupted by closing music)
Custodian does it twice in the short "Why Kitten Hates The Tau", once when Shadowsun leaves him and once at the end of the video, when he loudly and angrily declares that THIS IS NOT CANON!. Played for laughs both times.
The omniescent shard of the Emperor screams a giant NOOOO! when he realizes that he can't contact the Throne-Emperor and warn him about the Tau. For laughs, just like all the rest.
The Nostalgia Chick does this on being exposed to the Christmas Shoes song in her "Top 10 Disturbing and Inescapable Christmas Songs" special. The Nostalgia Critic also shouts this frequently. A notable one is when he finally finds out that The Star Wars Holiday Special does, in fact, exist... and that he has to review it.
Nostalgia Critic: No... it's not true... it's impossible!!! Darth Vader: Search your feelings, you know it to be true! Nostalgia Critic: NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!!!!!!!!!!
The Nostalgia Critic has "I HAVE A NEGATIVE RESPONSE TO THIS SCENARIO!!! I HAVE A NEGATIVE RESPONSE TO THIS SCENARIO!!!" This while making fun of Tombstone's Big No. However he actually lists this trope as one of his favorite clichés.
Rob (dressed as Emperor Palpatine) tells Doug (dressed as Darth Vader) that he must review the 1999 animated movie The Legend of the Titanic, leading to a pastiche of the scene where Darth Vader yells "NOOOOOO...".
Critic: NO! [pulls down technicolour swirly image over the camera, bearing the word "NO!" in big red letters, then waves a hand in front of it] NOOOOOOOOO!
Nostalgia Critic also discussed it in the Top 11 Coolest Cliches as "Screaming 'No!'"
In The Batterys Down Courtney has one of these when Jake says he's quitting musical theatre.
Also from a Let's Player, Chuggaaconroy's occasional Epic No. To be fair, it isn't a long, drawn out "NOOOOOOOO" as much as a deep, harsh "NO!" (in style of Dr. Robotnik's "NO!" in Adventures of Sonic the Hedgehog), which in context is a rejection, not the cry of despair seen in movies/other media and is usually said to show what saying no in a dialogue option will merit. Chuggaaconroy parodies himself in this video at about 11:46. "And there's Luigi's Epic NOSE!"
Though he does have "cries of despair" frequently, particularly when he's joined with the rest of The Runaway Guys. How many? Three minutes worth.
"Unfortunately for Junior, he's getting shipped to St. Understated, Thoughtful Conclusions Academy. Life's gonna be rough."
Also a quieter-than-usual (but angry) example as an opinion on planking.
The Annoying Orange: Orange at the end of More Annoying Orange and Cheese at the end of A Cheesy Episode. Pear also shouts this numerous times in slow-motion in "Forbidden Love'' when attempting to stop romantic embraces between couples as a can of cola and a pack of Mentos, a balloon and a pin, etc.
Spoony reacts this way in the "Party Mania" review first to suffer an eternity of a party in Canada and the second time when he will lose his 'wiener'.
He mocks the use of one in Tekken: Blood Vengeance, arguing that they hadn't really reached the Noooooo! stage of a relationship given their three minutes of screentime.
Two in Water-Human, both very dramatic and ending abruptly. First, the Water-Human cries out over David Hasselhoff's corpse, before realizing that it's weird to angst so much over a guy he knew for less than a minute. Later on Water-Human and a prison warden cry over the Large Beetle, who claims to have a heart attack, but then reveals he was just kidding.
Marik: I'll just work on my tan. Wait. I'm a vampire. I no longer have a tan. NOOOOOOOO!
The end of the "Sloppy Roethlisberger" episode of Epic Mealtime has Harley accidentally breaking and spilling an entire bottle of Jack Daniels, with this being the appropriate response from someone in the background.
A couple of times throughout Nakar's Let's Play Ultima series, but most memorably right at the end of Ultima VI.
Beh Lem: To be saying nooooooooooooo in slow motion! Death Cannon:*BOOM*
The Ladybug from Last Dance of Ladybug also gives one after she was injured by a kid.
Twice in Two Best Friends Play. One was Pat's response to the thought of paying for all of Vampire Rain, and the other is when Matt found out they were going to play Shaq Fu.
Gronkh often shows this reaction, for example on various occasions when being killed in Minecraft, (most often by a creeper, for example here).
TomSka has a NO! moment while discussing the post-production of the then-impending video "Holy Shit, Cats!"
Tom: ...then I have to send it over to Andrew, who puts the special effects in—... Jenny:(produces Tom's "Dubstep Sunglasses" and puts them on. Pop music plays instead) Tom: NO!! NOOO!! NOOOOO!! (video cuts to the end) * In the Team Four StarLet's Play of the Left 4 Dead 2 custom campaign "Detour Ahead." Just after barely surviving most of the finale, Gan gets two in:
Lani: OH MY GOD A TANK! Gan: NOOOOOOOOOOOOOOOO! NOOOOOOOOOOOOO!
Said tank promptly killed Kaiser and incapped the rest of the team within 7 seconds of Lani's warning.
The Canadian Star Trek web series parody, Star Track: Idomo has some Big N Os!
Captain Tremblay said it when the Blorg destroyed his planet, and when Geordi La Farge dies in main engineering.
Kuval screams it when the Christmas Messiah is beamed away.
The Orion captain screams it when Tremblay kills him.
The evil genecist screams it when Yeoman Bland destroys all his clones or (his calones).
Rhian screams it when Kuval rams his bird of prey to his warbird.
Captain Fortune screams it when his wife dies.
One episode of Game Grumps had Arin doing this after JonTron calls a Pokemon by the wrong name.
Jon: Oh, it's a Pumbloom!note It's actually named a Whismur. Arin: Do you remember the episode where Ash catches a Pumbloom? Jon: Dude- Arin:(angrily)NO!!!
Arin gets another one in Castlevania, after a skeleton enemy accidentally leaps into a bottomless pit.
In I'm a Marvel... And I'm a DC, Darkseid gets one after discovering he is a fictional character and an action figure in Zero Hour.
Atop the Fourth Wall: Linkara's reaction to some oddness involving Wolverine in Marville is "One, no. Two, God no. Three, that was a terrible pun. Four, NO NO NO!"
Todd in the Shadows: his response to imagining the Black-Eyed Peas' "The Time (Dirty Bit)" redone with other eighties music is to scream "NO!" with increasing volume after each clip.
In How to Start off 2014 Right! by Matt Santoro, Matt calls a random stranger and asks her if she wants to be his friend. She shouts, "NO!".
Anna Akana does this twice in "APPRECIATE ART (TV is awesome)". The first time occurs after her mother turns off the TV during the Secret World of Alex Mack finale, sending her to bed before she can see the last five minutes. The second time comes when Anna asks Alex Mack star Larisa Oleynik how the show ended, only to learn that the finale closed with a cliffhanger.
Episode 5 of Wall Turkey's Starving Saturdays (which is about the Hunger Games Simulator) focuses on the Genesis Wars, and John and Ben watch as the 32X is one of the remaining three consoles. John says that he will be mad if the 32X wins this. John's words become a reality as the 32X wins. John then screams this.
In the Regular Car Reviews of the 1987 Jaguar XJ 6, the review ends with Mr. Regular posing a question:
"But, should you own a 1987 Jaguar XJ6?" "No. No. No. No. No. No. No. No-no. No No No (repeating) UGH NO! NOO! NO DON'T EVER, DON'T DO IT!"
He lets out the Darth Vader one when seeing the dance mode in Kinect Star Wars. It even blows up Admiral Ackbar's ship.
He gave out such a huge one in his Blood Bowl match against Wowcrendor when he rolled 3 Attacker Downs that Crendor gave him the Broken Microphone Award.
He gave one of these in his attempt to stream his playing of the PC version of Batman: Arkham Knight when the game crashed. Again.
During the Far Cry 4 co-op when he learns that his pet elephant was killed.
He gave one in his vlog about the second Batman v Superman: Dawn of Justice trailer when he sees Doomsday, then proceeds to go on a rant about the use of the character and drops an Atomic F-Bomb at one point. The video description is also one of these.
This trope is one of the sins on CinemaSins. Whenever it happens, the narrator responds with "No" *ding*.
Christopher Odd lets off an epic one during Bloodborne after Vicar Amelia kills him just before he can finish her off. It lasts for thirty solid seconds.
During the Mario Party 2 finale of Pirate Land with Space Hamster and Pro Jared, the three talk about who the winner is. Space Hamster is the one to come out on top and take on Captain Bowser. PBG and Jared let out a Big "NO!" while Space Hamster lets out a Big "YES!", proclaiming that the curse is broken.
DSBT InsaniT: In 'The Camping Webisode': Koden when he discovers the campsite was trashed, and Duck when Martha accidently pops Balloon.
Left POOR Dead: Reginald has not one but two of these during Tippy's 'death' scene
Critical Role: While the name of the current adventuring group "The Mighty Nein" is often comically misconstrued as describing the number of members (say it out loud), it could actually be taken to translate as "The Big No".
Belkinus Necrohunt: The major fight that takes up most of Session 12 concludes with Thyrmelius Cog shouting this as Enoch impales his head with a rapier.
How can you talk about this trope without talking about Chuggaaconroy?
The Editing Room's Abridged Scripts of Marvel Cinematic Universe movies usually have someone exclaim this after the Snap takes away someone dear. Played for Laughs when somebody outside of the MCU — such as the Marvel Marketing Department, distraught at losing at least one of their biggest cash cows — delivers the Big "NO!" instead.
GeorgeNotFound says this when he gets killed by Dream in Minecraft Manhunt.
When Wookieepedia, the official unofficial Star Wars Wiki, was going for their first Featured Wikia of the month on the Wikia main site, there was some consternation when they discovered they were losing to WikiFur, the Furry Fandom's Wikia. This sent them on a wild scramble for more votes. The furries, to their credit, played up their role of villain with the following message on the Wikia boards. "Join us, and together we can rule the Internet as Wookie Fur!" Wookieepedia made the only appropriate response. If only Chewie, rather than Vader, had delivered the last Big No of the saga....
When the Whispering Forest appears in episode 23 of Welcome to Night Vale, officials from the town's Parks Department react with a press release consisting of "the word 'no' on a single piece of paper, but with hundreds of Os and maybe two dozen Ns."
In Part 3 of Pinkie Pie's Exciting Adventure, when the Unicorn is beaten by Pinkie from a reflected magic blast. (Though the sound clip is taken from Dota 2)
One day on Yahoo! Answers, a user by the name of Susan asked a question that would become memetic: "Does spider have puss puss?" The response, from someone going by Sisyphus, was simply "NO!!!!!!!!!!!!!!!!!!!!!" Whether Sisyphus was Screaming at Squick, attempting Translation by Volume, or just being a typical Yahoo Answers user, the world may never know.
James A. Janisse from the Dead Meat YouTube channel has a few moments:
When his original prop ax from Mandy (2018) breaks after he finished filming the Kill Count for said film.
James A. Janisse: Wait... what? What's going on? That clip's not from Hellraiser: Bloodline. It almost looks like- (turkey gobble) James A Janisse: Oh no... (turkey gobble) James A Janisse: Oh God no! (turkey gobble) James A Janisse: Motherfucker!!! Turkie (from Thanks Killing): Nice tits, bitch! In space! James A Janisse: NOOOOOOOOOOOOO!!!!!
Grian: Wait, is, are we-? No. No, no, no, I didn't just take damage, did I? Joel: I dunno, Scar- Grian: Scar, I think- Scar?! (Meanwhile, Scar is distracted by an allay) Grian: Scar, I think we're soulmates and you're too busy chasing fairies!! Joel:(punches Scar) Did that hurt you, Grian? You ready? (punches Scar again) Grian: No. Not aga- (takes damage when Joel hits Scar with an axe) NOOOOOOOOO!! Joel:(snickers in disbelief) Are you actually linked?!
Bonus points in that Scar was within earshot of that, had Grian following him around practically all episode since that moment, and was still completely unwary of this until the end of Day 1 — and even then, only because Grian told him to his face, and that alone took him two times since Scar got distracted by pandas, which his custom resource pack styled after his real-life cat Jellie.
Scar: …Oh my God. This whole time?!
This 6 second YouTube video titled "Yelling Creature" by Rob Herman.
In thisYouTube Poop based on Pretty Cure, upon hearing a shipper suggest a romance blossoming between him and Haruka, Aroma screams one of these, which gets cut off by the scene change.
helpfully provides a Big No button for use "in dire situations".