Can happen in 24 quite often, but Jack Bauer gives a couple good ones on Day Four after Habib Marwan kills himself. He was needed alive, as he was the only one who knew where a nuclear missile was currently headed within the country.
Played straight (and generally approved of) near the end of Angel's Season Three. Possibly helped by the hero sobbing so hard he can barely get the word out.
In the Are You Afraid of the Dark? episode "The Tale of the Renegade Virus" a virus attempts to enter Simon's brain, via plugging in the serial port embedded in Simon's hand, leading Simon to scream "NO!" The virus also does this a few times after Simon defeats him by "pressing any key to escape".
One girl does this in "The Tale of the Many Faces" when she is threatened with the "Punishment Book".
Ashes to Ashes (2008), when Alex fails to prevent her parents' death, compounded by the knowledge it was her father who had done the deed.
In the Season 4 episode "The Face of the Enemy", an increasingly upsetting season-long arc culminates in a brainwashed Mr. Garibaldi betraying his friends in a big public way, leading to the capture and torture of the captain. Guess what happens when Garibaldi snaps back and realizes that everything he loves is gone and all his friends believe he betrayed them?
Also, this particularly gratifying exchange earlier in Season 4, with Morden finally being broken of his smugness:
Mollari: Your ships are very impressive in the air, or in space, but right now they are on the ground. Morden: So? They can sense an approaching ship from miles away. So what are you going to do, Mollari? Blow up the island? Mollari: Actually...now that you mention it... Morden: NOOOOOOOO! (Londo presses a button on a small remote detonator he takes out of his pocket, and a distant explosion is heard)
A surprisingly effective one (and rare foreign-language variant) is heard in the 1967 Batman (1966) episode "The Mad Hatter Runs Afoul." At the start of the episode, Batman and Robin, having been placed in a fluoroscopic cabinet by the Mad Hatter's goons, appear to have had the flesh fried off their bodies by deadly radiation (when, in fact, the costumed skeletons are just dummies set up by the Dynamic Duo as a ruse against Hatter). Commissioner Gordon and Chief O'Hara shed Manly Tears when they hear the supposed news, and then get the word out to various world leaders, all of whom are momentarily absent and having their calls taken by a Sexy Secretary of the respective nationality. The Soviet secretary's reaction is probably the single most genuinely dramatic moment in the series' history:
In Battlestar Galactica (2003), Lee Adama gets a very effective one when Starbuck is apparently killed somewhat spoiled when they used it in recap clips for the rest of the season.
In the Big Time Rush 2-part special Big Time Concert, when Gustavo hears that James already left for LA with Hawk, he begins to scream a Big No, but briefly runs out of breath, stops, and takes some water from Kelly. Then, after he drinks it, he continues to scream so loud and the view shows that it can be heard from space.
Parodied on Big Train when Chaka Khan has a western-style shootout with The Bee Gees - when Robin Gibb (1849-1880) is gunned down by Chaka Khan (1850-1920), Barry Gibb (1847-1880) gives one up that lasts nearly half a minute. Then he does a second one. (During that time, Robin takes upwards of 70 bullets from Chaka's six-shooter. Rule of Funny in full effect, of course.)
Big Wolf on Campus makes fun of it. When an attempt to save Merton's life seems to fail and Merton just lies still, everyone goes quiet... and then Merton goes "That's it? No tears? No wailing? No protracted moan of nooooooooooooooooooooooooo?"
Ilana does this in the Broad City episode "Bedbugs" when she realizes her mattress is infested with bedbugs.
Played with in Brooklyn Nine-Nine, in which the Big No is actually more of a Long No. After Jake learns that he's inadvertently invited Amy's boyfriend Teddy to join her for a romantic evening without realising that Amy intends to dump him, Jake's perfectly restrained when he says "Oh, no!", and says it at a perfectly reasonable volume. But he makes the 'no' part last for at least ten full seconds (and possibly even longer, since he only gets cut off by the commercial break starting).
Parodied in "Storyteller". When Andrew is trying to convince Buffy—and himself—that his murder of Jonathan was actually accidental, or he was possessed both his imagine spots end with an over-the-top Big No.
The Master does one of these at the end of "The Harvest" when Buffy kills Luke, ruining the Harvest.
Collin's last words.
Combined with a rather twisted Aw, Look! They Really Do Love Each Other, Amy lets out a big no when the Seed of Wonder is destroyed and she loses her powers, causing Warren to fall apart in front of her eyes, since he had only been held together by her magic.
As Rebecca goes into her office, Woody is about to leave the bar and tells her he's going out to ask her boss' daughter out for a date. Rebecca, not really listening, says "Okay." and closes her door. Then a second later, she runs out of her office and across the bar, screaming "Nooooooooo!!!!", jumps on Woody's back and tackles him just before he reaches the door.
In one episode from the last season, Carla has one (it lasts through an entire commercial break) when she realizes that she slept with Paul.
In Chuck, Sarah lets a loud one out when Michael Clark Duncan's character drops Chuck head-first off the roof of a building.
Chuck returns the favor in slow-mo as he takes a bullet meant for Sarah in the series' penultimate episode.
Community has several first-season "mini episodes" that take place during study breaks. In one, Abed asks Pierce, the creepy offensive member, to play Truth or Dare. When Pierce chooses Truth, Annie yells, NOOOO. She calms herself and apologizes. Then Abed asks Pierce what he's ever done that he's ashamed of and the whole group yells NOOOO.
In "Advanced Criminal Law", when Chang is about to announce that the entire class will receive a zero for a quiz since the person who wrote the crib notes he found didn't fess up, Annie screams, "NOOOOOOOOO!" When everyone looks at her, she says she's never gotten a zero before.
In "Remedial Chaos Theory", the alternate timeline where Troy goes for the pizza ends up being the darkest one; when he returns, the place is in chaos, the Serbian rum catching the apartment ablaze, Pierce with a fatal bullet wound, everyone freaking out, and Pierce's troll figurine in the middle of it all, leering at Troy, who hollers "NOOOOOOOO!!!"
Played for laughs in the Britcom Coupling twice: when Jane finds out her boyfriend is opposed to premarital sex, and when Patrick encounters 'The Melty Man'.
Used to great effect on The Daily Show during, of all things, the 2000 election aftermath. At the end of a long montage involving the host and correspondents working through 37 days of hell, during which they turn to cannibalism and Jon and Stephen apparently fall in love, Al Gore concedes and it looks like it's all over — when suddenly Stephen is shot dead. "NOOOOOOOO!!!!!"
The Even Stephven segments occasionally had these when Carell and Colbert were explaining their positions:
"No!" "Yes!" "NOOOOOOOOOOOOOOOOOOOOOOOO!"
Stephen Colbert regularly uses them for comic effect. He is well known to be a huge fan of both Star Wars and The Lord of the Rings, so it's obviously an homage. For example, in his "audition tape" for the post of White House press secretary, where Colbert repeatedly uses this as he is pursued by Helen Thomas.
Daredevil (2015): Played for laughs in the season 2 premiere. The Nelson & Murdock trio is playing an after-work game of pool at Josie's. Foggy at one point leaves to use the bathroom. While he's away, Karen takes this time to teach Matt how to play. Karen has just helped Matt line up a shot, and he takes it, just as Foggy comes back from the bathroom, causing Foggy to exclaim "NOOOO!!"
Matt Murdock: That good, huh? Karen Page: You sunk the eight-ball. Matt Murdock:[grins]Well, something went in. Karen Page: I tell you what, you boys re-rack. I will, uh, buy rounds for the rematch. [Karen heads off to the counter; an annoyed Foggy turns to Matt] Foggy Nelson:I leave you two alone for ONE minute! Matt Murdock:She wanted to teach me![Foggy smirks at him]What?[Matt takes another turn, and manages to sink two balls at once] Foggy Nelson: Showoff... [Matt laughs]
The Dark Crystal: Age of Resistance: Rian gets multiple ones in the first season, one from watching Mira's essence being taken and the last bits drunken by the Skeksis Chamberlain and the other from watching his father perform a Heroic Sacrifice to keep the Hunter away from his son.
One of the more effective uses of this in the old series is in "The Three Doctors", when Omega has only just realized that he is little more than a walking ghost partly because his denial is also followed up by a very tortured primal scream that sounds disturbingly realistic.
"The Ultimate Foe": The Sixth Doctor screams a Big No when he is slowly disappearing into sand with hands grabbing him.
In the TV movie by FOX, the Eighth Doctor yells it when the Master kills Chang Lee and again when the Master kills Grace Halloway.
"The Age of Steel": After being turned into the Cyber-Controller, John Lumic does this twice: once when the Doctor and Mickey put an end to his plans of planetary conversion, and once when he's falling to his death.
"The Idiot's Lantern": The Wire's last words before the Doctor traps it on a videotape, preventing it from stealing millions of faces to regain physical form.
Used very effectively in "Doomsday", though it isn't so much heard as conveyed though Ten's expression, when Rose loses her grip and is nearly sucked into the Void Between the Worlds.
"The Runaway Bride": The Racnoss Empress' reaction when the Doctor uses the contents of the River Thames to drown her ravenous children.
Used at least twice in "Voyage of the Damned" by... go on have a guess from the sacrifice made by Foon Van Hoff to stop one of the Host as well as Astrid when shetakesMax Capicornwith her into the nuclear engine. The second was also unheard and accented with music just like "Doomsday".
"The Fires of Pompeii" has at least two characters Big-Noing simultaneously at its climax, the High Priestess and Lucius.
"The Impossible Astronaut" has not one, not two, but three Big Nos: The first, from Amy as the Doctor is shot. The second, shortly after from River as the Doctor is shot mid-regeneration, killing him for real. The third, from the Doctor as Amy shoots the astronaut without realising who it is.
"The Crimson Horror": Mrs. Gillyflower does it a few times, one example being when Clara jams the controls for the rocket carrying the Red Leech venom with a chair.
The Twelfth Doctor yells a gigantic one to the heavens in "World Enough and Time", when he is reluctantly regenerating. It's actually much more saddening when the context is revealed in "The Doctor Falls".
The cliffhanger ending of season 2 of Farscape has left Aeryn dead, John an unintelligible vegetable on an operating table, and Scorpius walking away having killed the doctor and recovered the neural chip from John's head. He tries to let out a big no. As his speech center has been carved out along with the chip it's more of a "FWWOOOOOOO", but the effort was there.
Barry screams one of these when Blackout is about to kill Dr. Wells. The emotional surge reawakens his powers and takes them Up to Eleven, allowing him to outrun Blackout's lightning bolts.
Killer Frost screams one as Cicada is about to stab Barry, awakening and blasting the villain with a cold blast. The big shock here is that Killer Frost still has her powers despite Cicada's power-draining dagger.
Played for laughs in Frasier in Part Two of "Shut Out in Seattle" — and in slo-mo! — when Faye asks Cassandra's name, undoing Frasier's double-episode-long Snowball Lie that was keeping his relationship from exploding. He also dashes across Cafe Nervosa in slo-mo as he shouts it, scattering tables along the way, but doesn't get there in time to avoid disaster.
Monica gives one on Friends when she and Rachel lose the trivia contest and have to give their apartment to Joey and Chandler as a result.
Right after Joffrey gives Ser Ilys the order to execute Ned Stark for treason, a witnessing Sansa screams several of these in her Freak Out.
In the last seconds of the episode "A Man Without Honour", Maester Luwin upon seeing what he believes to be the charred corpses of Bran and Rickon Stark.
Cersei when Tyrion arranges for her daughter Myrcella to be shipped off to Dorne.
Brienne yells this in "The Ghost of Harrenhal" after Renly is stabbed through the heart.
Stannis' attempt to rally his routed troops at the end of the Battle of the Blackwater quickly devolves into this.
Brienne's desperate screams as she is about to be raped by Locke and his men in "Walk of Punishment".
Parodied to the max in Garth Marenghis Darkplace, in which practically every dramatic / tragic moment in the series will be punctuated by someone — usually Rick Daglass — screaming "Nooooooo!!!" at the top of his lungs, in slow motion, for at least a full minute. Usually more than once.
Green Wing: Sue White's reaction to Mac withdrawing from the slave auction.
In the season 3 finale of Heroes, Angela Petrelli gives a (possibly) frightening Big No upon seeing the bloody corpse of Nathan.
Highlander plays it straight with Duncan MacLeod shouting "DARIUS!" upon finding his dead body.
Duncan delivers one in the episode "Star Crossed" when Kalas has defeated and is about to kill Fitz.
Blah Blah makes Ted say moderately big Big "NO!" when she blackmails him into telling Marshall and Lily that he (Ted) remembers their romantic meeting with Love at First Sight and similar sweet stuff quite differently, and that he and Lily were actually making out at a party.
Barney has a habit of doing Big No to close episodes. For example, when he learns that Marshall started a web page which is a count down to his next slap (Marshal having won the right to slap him five times whenever he chooses in a bet).
When Barney gets banned from Laser Tag, only a few tickets away from winning the remote-controlled helicopter, he drops to his knees and lets one out as he pulls a long string of tickets from his pockets.
Barney gets a silent one (complete with a skyward glance and camera pullout) in the beginning of "The Final Page" when, after two years since his last encounter, he is silenced by Marshall calling him on a jinx.
Parodied in "The Magician's Code":
Barney: What am I gonna be coming home to? (fantasy) Quinn: How would you like to have an eight-way with me and my six hottest stripper friends? (real life) Robin: How is that a punishment? Barney: Wait for it.... (fantasy) Quinn: But I forbid you from filming it with your overhead camera that zooms in on whoever's being the loudest. [Barney sinks to his knees, and the camera zooms in on his "NOOOOOOOO!"]
Kamen Rider Gaim: Kouta has one upon not being able to save team Raid Wild leader, Hase from being killed.
Kamen Rider Build has a belt that normally punctuates his transformations with a loud "YEAH!" until he gets the Hazard Trigger adapter for it, which causes it to instead let out one of these. Given what the resulting form is like, it's easy to see why it would prompt such a radical change of heart.
In "Flame's End", when MacGyver's New Old Flame's car blows up, the action slows down as MacGyver rushes toward the car, the soundtrack mutes, and MacGyver shouts her name.
When, in the Very Special Episode "Blood Brothers", Jason Priestly's character accidentally shot himself, things went slow-mo and MacGyver yelled not that character's name, but the name of his childhood friend who died after accidentally getting shot.
Magnum, P.I.: In "Echoes of the Mind", Magnum lets out one as the Dupre sisters (played by Sharon Stone) kill themselves off screen in the last moment of the episode.
Merlin has one (well, two close together) at the very end of season 1, when he thinks Gaius is dead.
Also Sir Leon in series 3 when Morgana has her men shoot into a crowd of innocent citizens after the knights refuse to swear loyalty to her.
Miami Vice episode "Calderone's Demise" features a string of no less than eight successive iterations of the slow-motion Big No.
During a Mystery Lab segment on Mystery Hunters, Doubting Dave, having showing how to make a homemade metal detector, drops the key for a chest of gold coins down a drain grate and then accidently closes the chest. He then proceeds to give a Big No.
Notable in one episode where Ned starts yelling NOO! in a classroom before the opening credits begin and is still yelling it afterwards at Moze's locker he is still yelling NOO! until she shoves a sock in his mouth.
Michael has this reaction when he discovers Toby has returned from Central America. The fact that it's interrupted by the opening credits makes it funnier. Behold.
Classic one with Pam after discovering Michael is sleeping with her mother. Like the example above, made funnier by a break; in this case, she runs out of the office in a Big No, and the show soon cuts to a commercial break and upon return, she's still in the midst of her Big No in the parking lot.
Dwight gets a truly epic one when he gets the news that a billboard with himself and Andy in a rather unfortunate position has been defaced. In all it's glory.
Played straight in Primeval. In episode 4 of Season 2, Plucky Comic Relief Connor attempts to get around a particularly nasty creature to rescue his friend/crush Abby. The creature smacks him across the room, where he hits a wall and then drops out of sight, resulting in Abby uttering the Big No. While it was probably Narm to some viewers, actress Hannah Spearritt deserves serious credit here. The anguish on her face and in her voice actually makes the line work.
Daniel Grayson gets one in the Revenge episode "Atonement" when Kate, FBI agent and daughter of Big Bad Malcolm Black, is about to shoot a beaten Emily - he gets in front of Emily, and Kate shoots him. Jack bursts in very shortly afterwards and takes down Kate, but by that time poor Daniel's become the latest example why on RevengeAnyone Can Die.
Saved by the Bell: The New Class: Played for Laughs when it takes place while the cast is at space camp in the Season 4 episode "The Fifth Wheel". Eric is supposed to be in a simulation space shuttle with a girl he has a crush on, but, on the day said simulation is set to take place, she becomes ill and can't attend, so Screech and Mr. Belding go in her place. Eric is unaware of the changes until Screech and Mr. Belding walk into the simulation shuttle.
Eric: *off-screen* Screech?! Mr. Belding?! NOOOOOOOOOOOOOOO!!
Skins has a particularly odd one, when Sid wakes up after an alcohol-induced stupor to find pictures of his ex-girlfriend kissing another boy taped to his entire body.
Used as the season one cliffhanger of Sliders (when Quinn is shot and then cradled in Wade's arms — Sabrina Lloyd can scream), and again in season two (when Wade dreams of her past-life self being shot and cradled in her lover's arms).
Parodied when Tim screams "NOOOO!!!" at the top of his lungs when cradling the body of his fallen best friend Mike, who has just been shot whilst saving Tim from an ambush by a hated rival and has just given a moving eulogy. It's worth noting, however, that this is merely a paintball game, and Mike, having merely been shot by a gob of paint, is far from dead; indeed, the scene immediately cuts to the two of them leaving the paintball centre casually chatting about how much fun they had.
Later parodied again in the first episode of the second season, in which Tim, recounting events in voice-over, introduces Mike — who runs to the centre of the pavement, falls to his knees, and angstily screams "WHYYYYYYY?!?!?!" at the top of his lungs for absolutely no reason whatsoever. According to the Spaced Season 2 DVD'S Homage-o-meter, this moment is a reference to Platoon.
Another one, when Tim fails to prevent Tyres from delivering his portfolio to a prospective employer (having learnt that it still contains an unflattering drawing of said prospective employer, having earlier turned down Tim's work, describing himself as a 'massive wanker'). Tim's dramatic Big No is rather undercut when Mike runs up seconds later asking with cheerful obliviousness whether he's managed to retrieve it. He and Tyres then ignore Tim entirely and start chatting amiably.
In the season 1 episode "The Alternative Factor", Lazarus lets out a Big No when Kirk vanishes into an antimatter universe.
In the third season episode, "Whom Gods Destroy", Kirk does this three times after he is forbidden to beam aboard the Enterprise after Scotty tells him to answer the chess problem. Turns out it is actually Garth of Izar in disguise.
In the Season 6 episode "Timescape", Picard succumbs to temporal narcosis after laughing, he repeatedly lets out a Big No.
In the 3rd season episode "Sarek", Picard yells it after the mind-meld with Sarek.
In the 6th season episode "Frame of Mind", Riker screams a Big No before waking up from a nightmare.
Counsellor Troi screams a Big No in her hallucination in the 5th season episode, "Violations" and again as an old woman when the alien ambassador heads for the transporter room in the 6th season episode "Man of the People" and once more, when Data stabs in her shoulder in the turbolift in the 7th season episode "Phantasms".
In the 1st season episode "Hide and Q", Q does this when he is called back to his people. Worf does it earlier in the episode when a Klingon woman appeared and wanted to have sex with him.
Picard gets another Big No moment in 4th season episode "Night Terrors," when he hallucinates that the turbolift ceiling is bearing down to crush him.
Data does it when he punched Picard in the 2nd season episode "The Schizoid Man."
In the 5th season episode "Darmok", Picard does this when the transporter snatched him away.
Wesley Crusher screams it in the climax of the 7th season episode "Journey's End".
Hugh does it at the climax of the 7th season premiere episode "Descent, part 2" when Lore is going to kill Data.
Worf gets a Big No in 1st season episode "The Last Outpost," and Riker does it a moment later.
Chaotica: Your once proud country will fall to its knees. How ironic that I am using your rocket ship to lead my space force into battle! Kim: You're wrong, Chaotica. Before you came on board, someone pushed the self destruct button. In three minutes, we'll all be dead. Chaotica:(with appropriate Large Ham body language) NOOOOOOOOOOOOOOOOOOOOOO!
In "One", Seven screams this trope after the Doctor goes offline, leaving her to deal with the hallucinations she's having, plus the fact that every system is failing by herself.
In "The Thaw", Kim screams "NOOOOOO!!!!" when the Clown is about to cut him with a scalpel before being stopped by the Doctor.
In "Remembrance", Picard yells out "No!" when Dahj is blown up in front of him.
In "Broken Pieces", Seven of Nine as a Borg Queen shouts a Voice of the Legion "NO!" when the unprocessed Borg are ejected into space. Not only did she lose thousands of drones, she could feel them die because her mind was connected to theirs.
In "Folsom Prison Blues", the guard who is killed in the teaser screams, "No..no...No! No! Nooo! No! No! Nooo! Aah! Nooooo!"
In "All Hell Breaks Loose", Dean lets out one when he just finally found Sam and seen him get stabbed in the back. Helped by the fact that the viewers are probably feeling the same thing and his look of complete panic and devastation at the sight of his little brother getting knifed.
Sam lets one out at the end of the pilot episode when Jessica dies.
This is Gordon's reaction in "Fresh Blood" as he's being turned into a vampire.
Dean reacts this way in "Swan Song" when Sam!Lucifer telepathically snaps Bobby Singer's neck.
On an episode of Top Gear (UK) in which Richard Hammond was driving the Bugatti Veyron in a two-way drag race against a Eurofighter Typhoon jet when said plane overtook him on the home-stretch to cross the finish-line first.
The Twilight Zone (1985): In "Private Channel", Mr. Williams gets one when he realizes that blowing up the plane would ruin other people's lives in the same way that his life was ruined after his wife and daughter were killed.
Played straight in Twin Peaks, when sheriff Truman breaks the sad news to Laura's parents.
V (2009) has not one, but TWO of epic proportions when Anna experiences her first human emotion in the Season 1 finale.
In The Vampire Diaries, a handful of characters did this, but without a doubt, Stefan's defiant "NO!" to Klaus in 3x05 takes the cake:
And again along with Maggie when the Governor partially decapitates Hershel in front of everyone at the prison.
Maggie wails one again in horror and grief when she witnesses Negan executing Glenn, and again with anger when Rick eventually decides to spare Negan.
Daryl yells one when trying to shield Carol from seeing that Henry, her adopted son, was killed and beheaded by Alpha.
Without a Trace. An FBI agent lets out one of these when a Middle Eastern doctor suspected of being a terrorist is sniped by the police, because the guy was actually trying to warn people about an impending attack and the agent had almost convinced him to surrender when he did something the cops mistook as a threatening gesture. Unusually, the audience doesn't hear the "No", but it's obvious from his body language and reading his lips.
In "Monday", Scully and Mulder are doomed to endlessly repeat the Big No, as they are trapped in a "Groundhog Day" Loop which always results in their deaths.
"This is Not Happening" has a heart-wrenching version of this trope, made all the more tear-jerking by Gilliam Anderson's performance. Scully has spent nearly a year desperately searching for Mulder, who was abducted by aliens. Finally, Mulder is returned, but not alive. The scene when Mulder's body is returned is emotional enough, with Scully insisting he needs medical attention and Doggett physically restraining her and dragging her away from the area. When Scully manages to break free, she runs at full-speed to find the supernatural healer she had been investigating; he had been bringing abductees back from the brink of death. She bursts into the compound to find that he and his followers are gone. Emotionally and physically exhausted, she collapses on the floor, screams a Big "NO!" and wails in anguish.