Subverted on American Dad! where the "hottest girl in school" and head cheerleader, Lisa Silver, appears willing to go out with the nerdy Steve and is surprisingly understanding when the show's antics keeps messing up the planned dates. In another episode, it looks like she and her Girl Posse did something terrible to Steve's unpopular girlfriend... but it turns out it is actually Steve's friends who are responsible, once again subverting what you'd expect from Alpha Bitch behavior.
Courtney Gripling is an inversion — Lovable Alpha Bitch. She's the queen bee; pretty, rich, popular, and more than a little narcissistic, a (self-proclaimed) snob, but she's a genuinely sweet person who doesn't seem to harbor any malice towards anyone — unlike her friend Miranda. While her sidekick Miranda is quick to insult the unpopular girls, Courtney is friendly with them.
Batman Beyond has Blade and Chelsea. Blade is actually fairly apathetic, but has the look down pat, and is more than content to do things such as throw most of a school assignment on Terry, or manipulate a classmate to make another guy jealous. Chelsea, is usually a lot more sympathetic; she's actually a rather nice person, as seen in "The Last Resort," and except for being dismissive of Howard Groote, doesn't really do anything bad. Though she sometimes tries to get Dana to see other guys, it's because she thinks Terry isn't good for Dana, due to him always brushing her off.
Claire Brewster in Beetlejuice who is still the "stuck-up, shallow yet very pretty girl" type.
Ciara Toler, when she was in high school and before she was brutally raped as a consequence of her bitchiness. While she showed strong Alpha Bitchy traits partly because of her status as "Barbee Creek Barbie" and "the princess of the projects" - the most attractive girl in her 'hood - her snobbishness is portrayed somewhat sympathetically as arising from her impatience with living in a community filled with people who preferred to wallow in poverty and being the only person (even in her own family) with a drive to improve herself. In fact, she often tried to act more like a Cute Sports Club Manager and uplift the spirit of her friends and neighbors before becoming exhausted with the fact they were only interested in drug dealing and violence.
Tammy Larson from Bob's Burgers is something of a subversion in that she thinks she's an Alpha Bitch and assumes she's super popular. The thing is, she doesn't have anywhere near as many friends or followers as you think she would if she were that popular. The only person who hangs around her on a regular basis and actually seems to like her is her Beta Bitch Jocelyn, and even she doesn't seem to like Tammy that much. The only other people who could possibly be considered her friends are Jimmy Jr., Zeke, and the Belcher siblings, and that should tell you something about her popularity level since they don't seem to actually even like her.
Boy Girl Dog Cat Mouse Cheese: Girl has a nemesis called Lila, who mockingly calls her "the queen of nerds" and threatens to shut down Girl's after-school cosplay club if elected school president, just to be mean.
Nina Harper from Braceface. Interesting, though, in that she and the protagonist, Sharon Spitz, were actually best friends when they were little. But a mishap with one of Nina's dolls that got its head popped off ended their friendship when she accused Sharon of being the culprit (even though she had no proof that Sharon did it). So her bullying Sharon is more or less out of spite. They do somewhat reconcile as the series goes on. Especially by season 3 when its revealed that Sharon was indeed innocent of the doll incident. Nina's cousin was the cause of that.
Dawn Swatworthy from The Buzz on Maggie. Unlike Maggie who does act selfish at times, she has zero redeeming qualities.
Cleo from Clone High. She is a very selfish, snobby, cynical, vain, manipulative, pretentious and materialistic stereotypical school diva who cares only about her social image, beauty and popularity.
Also, Cleo from Monster High is popular, vain, and frequently tries to manipulate situations to be about her. However, she does love her boyfriend and her friends, and occasionally displays redeeming qualities, becoming more of a Lovable Alpha Bitch as time goes on. Brilliantly Deconstructed in that her popularity is the result of her need for positive attention, which comes from her father and older sister treating her coldly and telling her about the importance of power, both of which went to Cleo's head.
"I'm Cleo De Nile, and I've got to give the people what they want."
Cleo De Nile, Boo York, Boo York
Elisabeth "Sissi" Delmas in Code Lyoko is a strange version of this. In the first season, this is played straight, with the exception of a few scenes in only a few episodes. However, her assistance to the heroes in later seasons, particularly the second and fourth, is often offered without second thought or a specific request for a reward, though this can happen quite a bit in a life-or-death situation. It seems that her personality alternates between seasons, from a straight Alpha Bitch with several Pet the Dog moments in Seasons 1 and 3 to a more Jerk with a Heart of GoldLovable Alpha Bitch in Seasons 2 and 4.
Paulina from Danny Phantom. The most popular girl in school and loves to rub it in people's faces.
The show also had Valerie Grey, who early in the show ends up losing her wealth and becoming a Fallen Princess. She still retained some bitchiness before completely mellowing out and becomes a villain towards Dannys ghost half.
Played With on Daria. You'd think the title character would have one as an arch-nemesis, but no, girls like Brittany are generally nice (if condescending) to her. Daria's sister Quinn, however, is part of the Fashion Club, the popular Girl Posse of their grade...and is in constant competition with its leader, Sandi, for dominance. Essentially, the two never have time to torture less popular girls because they're torturing each other instead. Quinn is generally the more sympathetic of the two, if only because she doesn't abuse poor Stacy, the group's least popular member.
Kimmie McAdams on Detentionaire, leader of The Glamazons, is the classic rich and haughty girl who sits at the top of the popularity food chain. Her mother is head of A. Nigma High's Parents' Council. However, we eventually learn that she wasn't always like this, and actually used to be close friends with Biffy (although the two may still have feelings for each other).
Beebe Bluff is a subversion. The richest (their middle school was named after her by her Dad), most popular, and somewhat snobby and spoiled, she was actually quite nice to everyone despite these character traits. She even ended up being Doug's First Kiss in a sweet way.
Jerk Jock Roger Klotz adds some Alpha Bitch traits after he becomes rich in the retool. Although Beebe does not want to get involved with him in any way.
Trixie Tang of The Fairly OddParents is rich and popular AND lusted after by everyone, including Timmy, the main character. However, in the episode "The Boy Who Would Be Queen," a genderbended Timmy learns that there is another side to Trixie — a boyish side that likes comics and video games, which would shock all of the people who worship her. She says that all she wants for her birthday is a friend who understands this, but when Timmy arrives at her party and makes the offer, she saves face.note It's possible that this side of her personality is hinted at in The Big Superhero Wish! and Wishology. In both specials, she apparently enjoys being in action scenes.
Lois Griffin was one back in high school, pulling a Chris Hargensen-level prank on a chubby girl. Interestingly, her subsequent life turned out much like Brian's aforementioned prediction of Connie's life.
Pacifica Northwest from Gravity Falls, which is later deconstructed in season 2 when it's suggested she's only this way because her rich parents are even worse and raised her to be stuck up. She begins to defrost as of "The Golf War", then "Northwest Mansion Mystery" reveals that she wasn't raised to be a bitch, she was mentally abused into being one. To put things in perspective, Pacifica was nothing more than a bully and a brat, but she isn't a sociopath. Dipper convinced her that there is still redemption for her.
Mindy from The Grim Adventures of Billy & Mandy is a much younger Alpha Bitch, as well as an incredibly obsessive one. For example, she loses her head cheerleader position to Mandy (who just shouts the game plans to the team) and, after consulting the "patron saint of cheerleading" (a trophy she thinks is talking), decides the best solution is to incapacitate her in some way in the middle of a match, or, failing that, blow her up. If she sees Mandy with anyone aside from Billy or Irwin or the like, she walks up to them, puts on an obscenely perky face, and says "You're my best friend!"
Trina Riffin from Grojband acts this way, even though she only has one dedicated underling, her much put-upon childhood friend Mina. Nonetheless, her official bio states her to be the most popular girl at her school (although since we rarely ever see the characters at school, this never pops up in the series).
Paige Logan from Grossology. She is a popular but very snobbish girl.
In Harley Quinn (2019), The Joker of all people is presented this way despite being an adult male; he's considered the #1 criminal in Gotham, to the point where he has more clout in the Legion of Doom than its actual leader. Using this popularity to his advantage, he takes every chance he gets to belittle, upstage, sabotage, and/or humiliate Harley when she dumps him and tries to make a name for herself, and he shamelessly manipulates her and toys with her emotions just for his satisfaction like a typical school bully would do. Furthermore, the Joker treats his fellow villains — including A-listers like the Penguin, Bane, Scarecrow, and Two-Face — as if they're nothing more than his devoted entourage and personal goon squad, as he casually bosses them around, insults them to their faces, plays annoying pranks on them, and steals food right off their plates.
Zoe, Audrey's abusive older sister, is a more obvious example, being a head cheerleader and the leader of a trio composed of her and her two friends.
Rhonda Wellington Lloyd comes between this and Rich Bitch in some episodes of Hey Arnold! However, she actually is shown to have a friendly side, since her best friend Nadine actually is implied to be a middle-class girl (and with almost completely different interests than Rhonda—Nadine loves arthropods, Rhonda loves fashion). Her romantic interest in Harold, who actually is fat and less popular, is another redeeming feature.
On the show Horseland, there are sisters Chloe and Zoey Stilton. They're definitely Alpha Bitches, and also Rich Bitch material. In "Bailey's New Friend", a new boy called Jesse arrives at the stable and is befriended by Bailey, whose parents own the stable. Zoey and Chloe use the new friendship to try and break up the friendship between Bailey and the five main girls. They do this for no other purpose than to see how miserable the girls will be without their friend.
Bonnie Rockwaller on Kim Possible, except she tries (repeatedly and in vain) to usurp the role of captain from the cheerleading heroine. Bonnie did eventually become cheerleading captain in one episode, and much to Kim's initial dismay, proves to be exceptionally competent and dedicated to the job (though she wasn't any less bitchy). Kim eventually decides to not fight it, as she's sure Bonnie will get sick and tired of all the hard work involved with being squad captain. And she's right; the next episode showing the cheerleading squad, Kim's clearly back in charge.
King of the Hill: Dale Gribble, yes, that Dale Gribble. While usually the weirdest and most pathetic of the guys, after Bill, a number of episodes will have Dale lead a clique with Bill and Boomhauer to actively bully and ostracize Hank.
Rachel. She is very popular among her classmates and she is a rude and snobbish Bitch in Sheep's Clothing who does horrible things such as locking Wendy and Marine out of their own house and throwing a party in it, or threatening to rip up a stuffed animal belonging to Marine, who is seven years old. She especially targets Wendy because Wendy is more popular than she is and the guy she likes is Wendy's boyfriend.
Rachel's mother also seems to be an adult version of the trope.
Wendy is an aversion. Despite being the most popular girl at her school, she's friendly and kind to her family members and classmates most of the time.
The Biskit twins from Littlest Pet Shop (2012). They don't appear to be the typical popular kids and are not outright hostile most of the time, but being rich, they are so pampered that as a result, they're distant from everyone else and are so used to getting what they want that they do not take kindly to Blythe declining their offer of friendship in the first episode. They become more openly antagonistic later on in season 2, but it gets dialed back when they get Morality Pets in season 3.
Lizzie has Brooklyn, whose sole purpose is to be the cool girl at school who treats Lizzie like garbage.
The Mighty B!'s Portia is the stuck-up, Valley Girl, blonde Alpha Bitch, with front teeth that are even uglier than the main protagonist's. Her cousin and mother are even worse.
Miraculous Ladybug has Chloe Bourgeois. Her father is the Mayor of Paris, and spoils his 'little angel' rotten. She hits all the marks for personality, but she is not very popular at all, with only one friend, Sabrina, who she treats more like a servant and also doesn't understand what friendship might actually be. She thinks everyone adores her, and can rarely accept she made a mistake. Among her most heinous acts is locking an emotionally insecure girl in the bathroom so she could be next to Adrien, who Chloe has crush on, in a class photo, which said insecure girl has no crush on. She is responsible for the most cases of people becoming sad or angry and getting brainwashed into supervillains, to the point that Hawk Moth once created an akuma with no specific victim in mind and had it follow her around since he figured she was bound to piss someone off eventually. Unfortunately, he caught her on the day she was specifically trying to be nicer to people, so it took a little while, but she eventually delivered.
Lila Rossi turns out to be one as well. She convinces the rest of the class, who adores her, to do whatever she wants and manipulates people to try and turn them against Marinette (the only person who doesn't like her). She also torments people for petty reasons, such as sending a picture of herself kissing Adrien to Kagami, or the aforementioned turning people against Marinette because Marinette refuses to believe her Blatant Lies. She also acts spiteful and horribly rude, insulting people behind their backs.
Princess Morbucks on The Powerpuff Girls. She's a rich, spoiled Prima Donna that thinks she's better than the girls. Nobody at her school likes her.
Princess Natasha: Greg's girlfriend Kelly is this, at least as far as Natasha is concerned. She is totally self=absorbed and her 'crimes' include wearing too much makeup and cheating off her fellow students.
Ready Jet Go! has a very strange example in Mitchell Peterson. First off, he's a guy, making him an Alpha Bastard. Second, even though he has a lot of the hallmarks of an Alpha Bitch/Bastard, like a rude attitude and popularity in the town, he doesn't have a clique of sycophants to boss around. He's also quite nerdy. Later, it's revealed that he developed a mean streak as a result of loneliness and shyness. He starts hanging out with Jet, Sean, Sydney, Mindy, and Lillian and becoming true friends with them towards the end of the show.
The Ashleys in Recess. Despite having some moments where they aren't a bunch of Rich Bitches, they spend the majority of the series wanting to screw with everyone for the sake of being mean.
Maimy from Robotomy. One of the most popular girls at Harry S. Apocalypse High and just as violent and hot-tempered as every other robot. In one episode, she started dating main character Thrasher (who has a huge crush on her) after he became student body president, but only did so to become vice president and overthrow him.
She's less of an Alpha Bitch compared to Savannah Shane, a girl whom even Angelica can't stand (and yet, she always seemed to be trying to get in her good graces). A particularly strong example comes from the episode when Savannah plans a party on the same day as Angelica's thirteenth birthday party, and it's found out that she did it on purpose to "teach Angelica a lesson" after sitting at her lunch table.
In The Simpsons episode "Eight Misbehavin'", the family describe what happened to them during a nine-month mid-episode Time Skip. Lisa reveals that she became the most popular girl in school, "but then blew it by being conceited". Whilst the details of this are never revealed, it is probable that she became an Alpha Bitch during that period.
Liz was introduced as one, mocking Peter as much as everyone else, until a boost of Character Development after seeing him in different light turned her into his love interest.
Sally Avril is the most abusive girl of the group, even mercilessly mocking fellow clique-member Flash Thompson when his hero, Spider-Man, appears to be committing robberies. Slightly subverted when Peter's aunt has a heart attack as Flash mentions that even Sally feels sorry for him, although she is not as forthcoming with her sympathies as his friends are. Both she and Flash have gone through a bit of Character Development. When she thinks that Peter's been killed, Sally is horrified. She does say it's because she'll have to tell Liz and Liz "looks awful in black", but when she sees that Peter's okay, she performs a textbook Anger Born of Worry. A bit later, she tells him that no, she doesn't care, but she doesn't want him to be blown to bits, she's not a monster. In the last episode, she's glad Liz broke up with Peternote When in reality Peter breaks up with her because of his feelings for Gwen Stacy, but publicly breaks Peter off to save face.
Squilliam from SpongeBob SquarePants is a rare male example. He's rich, has a few friends, and uses the fact that he is rich and successful to constantly taunt Squidward. On the other hand, Squidward almost always comes on top in episodes featuring Squilliam.
Gretel from Staines Down Drains is the arch enemy of Mary-Jane. She is a spoilt girl who usually bullies the Staines with her friends, the Lupe brothers.
From the first generation, Heather and Courtney. Both were the Alpha Bitch on their respective teams in the first two seasons with Heather serving as the show's main Alpha Bitch in Island and Courtney in Action. However, in World Tour, both girls clash for the position.
From the second generation, Jo, to an extent. Although Jo does not fit the "rich, popular, pretty girl" part of the trope, her commanding personality definitely fits with the "bossy, mean girl" part of the trope, and Jo clashes with Heather for leadership of the villains team in All Stars.
From the third generation, Amy and Sugar. Amy treats her twin sister Samey with absolutely no respect, and pretty much acts like a Jerkass to her other teammates as well. Sugar is a pageant brat who asserts herself as the dominant female, especially over Ella and Sky.
Taylor treatsher mom Kelly like shit, and she is often rude to the other contestants.
Josee is a rare adult version, given how she regularly bullies her partner Jacques and belittles other teams, primarily the Cadets and the Sisters.
Mandy from Totally Spies! is a textbook case of this trope. Extra points for being shallow.
Coral from Trollz is the head cheerleader, perpetually stuck-up, and is mean to the BFFL. She gets a case of Break the Haughty when Simon temporarily takes over and makes her his servant, and when Amethyst helps her she thanks the cast before returning to her usual ways.
A girl from Bloom's old hometown named Mitzi definitely fit the bill, despite appearing only a few times. On the Halloween episode, she invited Bloom and her friends to a party—which turned out to be an elaborate scheme to humiliate the girls. She bought and rigged a house, made up an elaborate legend, hired actors to pose as party guests (complete with scripts), and set up elaborate special effects around the house, just to pull a prank on someone she had barely seen in two years, along with four girls she had never met. Mitzi gets a more prominent role in some episodes of the fourth season: she lays her eyes on Brandon and wants to take him from Stella. Later, the Wizards of the Black Circle temporarily turn her and two friends into evil fairies. After that, she's practically Put on a Bus. She appears again in Season 5, and whatever time she's not playing fangirl to the Trix involves her being a bitch to her little sister.
The Trix themselves qualify. They bully others in school and generally boss others around before getting expelled. After episode nine, they get much, much worse, what with the multiple attempted homicides, turning The Cutie into a pumpkin for ruining one of their plans, removing the heroine's powers in a needlessly sadistic way after threatening her parents and revealing her backstory, and attempting to take over the universe. They're like Regina George, only with magical powers.