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1!All spoilers will be unmarked ahead. Administrivia/YouHaveBeenWarned!
2-->''"Killing's easier than you think. You just have to forget how sweet the sugar is..."''
3----
4* The very first episode has Tenma on the receiving end of WhatTheHellHero as a distraught widow blames him for the Director reassigning him from a Turkish construction worker to a famous Opera singer, rage and grief filling her voice as she dresses him down for it. Those around him are completely indifferent as he suffers a crisis of conscience over it, and his worldview gets a ''[[MyGodWhatHaveIDone harsh]]'' realignment during dinner with his fiancée.
5-->'''Tenma''': But what does she want from me, anyway? I was JustFollowingOrders from the Director when I performed surgery on that opera singer. [[YouKeepTellingYourselfThat I'm not responsible]]. (''To Eva'') Well am I?\
6'''Eva''': Well of course not! [[AssuranceBackfire After all, peoples' lives aren't created equal]].
7* Episode 2 is one long TraumaCongaLine for Tenma, as he's wrongly blamed for the mayor's death, Heinemann sabotages his career, Eva coldly dumps him, and he's removed from caring for Johan. His breakdown as he staggers drunkenly through the street ranting about his superiors is quite devastating.
8* Episode 3 shows the funeral of Dr. Heineman, Eva's father. While both are thoroughly repugnant people at that point, [[EvenEvilHasLovedOnes she's genuinely inconsolable over her loss]], to the point that Tenma tries to approach before she shakes someone else off.
9** She then arranges a lunch date with him, noting what he'd done at the funeral, and says she wants to give their relationship another try - [[HeelFaceDoorSlam he rejects her without a single word]]. While it may be deserved, it was undeniably cruel, and likely the last lifeline she had in the world.
10* Episode 4 introduces us to Junkers, a burgular who helped break into and murder several families. While repugnant, he seems to genuinely be remorseful, lamenting to Tenma about how this all started because [[SlowlySlippingIntoEvil he started lockpicking to afford a cuckoo clock he could never afford.]] Even as he escapes the hospital, he tries to save Tenma’s life by trying to warn him away from Johan, and whatever redemption he could have had is cut short by Johan executing him.
11* The death of Wolfgang Grimmer.
12** "So the Magnificent Stiener appeared." "[[https://www.youtube.com/watch?v=mUuUoJyeklo No, he didn't. I did it on my own.]]"
13** "I'm sad. I'm not sad that I'm dying. I'm finally sad that my child died."
14*** When Franz Bonaparta (freakin' [[RetiredMonster FRANZ]] [[TheSociopath BONAPARTA]]!) breaks down in tears, muttering [[MyGodWhatHaveIDone "What have I done...what have I done?"]]
15*** In a way, Tenma being the only one in the room that is ''not'' crying during Grimmer's death is equally sad when one considers his character arc throughout the series. Despite managing to [[RousseauWasRight retain a lingering idealism at his core]], his resigned stoicism in the face of it all really shows how having to endure so many repeated scenes of lives tragically cut short has turned him into a calloused shell of the [[GoodCannotComprehendEvil naïvely optimistic man]] he once was by that point.
16* Tenma pleading to a [[HeroicBSOD BSOD-ing]] Nina to not kill herself.
17* Additionally, Eva's DoomedAppointment with Martin. That one second of pure joy she experiences when she thinks that he actually ''did'' come only serves to make the scene that much more painful when she realizes the truth. Naoki Urasawa apparently [[{{EmotionEater}} feeds off of crushed hopes and dreams]].
18* Now, let's sit down and think-- [[SadisticChoice which twin did Johan's mother not want]]?
19** Even worse than that, though -- which twin did she believe would be better off?
20* Michael Müller atoning for his past crimes by choosing to save Nina from Roberto's men, and [[RedemptionEqualsDeath sacrificing the life that he had built with his new family]] in the process.
21* Once upon a time, there was a detective (Richard Braun). We saw him for just, like, three episodes, but we grew fond of him, after all. He had a lovely daughter that he was finally going to meet. He didn't drink anymore, he was getting his life together, and he had finally found peace. Then Johan happens, and something inside us dies; poor Richard just had to fall into his trap, just the night before he was ''supposed'' to see his daughter (which, unfortunately, did not happen). *Sigh* if only Richard had simply NOT answered the door when Johan came knocking; or, at the very least, just simply and politely refuse (and say he's busy or something) to go out with him! Of course, that is not to say that it is Richard's own fault that he got murdered -- afterall, Johan is a master-manipulator. And if Dr. Reichwein and/or Gillen had been able to contact Richard to warn him about Johan, would it have made a difference? Who knows... In the manga, they even name the 7th book after the guy and make him the main character in it... adding to the attachment and subsequent sadness. This MAY have been more bearable if Johan had been gruesomely killed at the end of the series, but...
22** "It's okay, you can have a drink now."
23* "Say my name." "We don't have names."
24* Nina... you bastard... why didn't you just... forgive... him?
25* Karl emotionally reading to Schuwald on the phone for what he thinks is the final time, which is quickly seamlessly married to a SugarWiki/{{Heartwarming Moment|s}} as Schuwald arrives and reveals that he knows Karl is his son.
26* Karl, Lotte, and Johan take Schuwald to his "special place"; a beatiful forested lake. Karl and Lotte try to disguise the fact that it's now a construction site, but Schuwald, despite being blind, still knows, as he can no longer hear the calling of the wrens. Johan, however, describes the spot so vividly that Schuwald is able to recall it in his mind. With tears streaming down his face, he says simply, "I can see it well."
27* When Jan Suk learns that Inspector Zemen wasn't the hero he thought he was, he keeps saying how much he admired him, and how he doesn't know what to believe in now.
28* Most people shed a few tears for Roberto when it's revealed shortly before his death that he was the boy Grimmer befriended in 511 Kinderheim. Made even worse when one realizes that since the children there decided to remember details about each other rather than themselves, it was very likely that Roberto knew Grimmer's real name.
29** Likewise, the SingleTear that Roberto [[CryForTheDevil sheds as he passes away]] on the rain-drenched ground, having been robbed of [[LetThemDieHappy his final (albeit nightmarish) hope]] to witness the "Scenery for a Doomsday" for himself after having [[BadBoss Johan]] [[KickTheDog coldly inform him that he will not be able to see it]] in his dying moments.
30* Speaking of Grimmer: "Doctor Tenma... What does my face look like right now?"
31* The ''entire'' Ruhenheim arc, because it could be known [[ForegoneConclusion knew what was coming]]. Ditto the elderly couple shown previously who show brief kindness and warmth to two lost, wandering children, and are killed for it.
32* In the last episode, Nina finds out she aced her thesis. After all that she had to endure, it was such a relief to see that her life was back on track, and that regardless of everything she'd lost, she ''would'' have a future.
33* Nina and Dr. Gillen visit Franz Bonaparta's cottage in Ruhenheim, whereupon it is revealed that Johan had already visited the cottage and perused through the various sketches of himself and Nina. Nina, somehow in tune with her twin brother's emotions in light of their folie a deux-like tendencies, states that he cried over the portraits, possibly indicating the first exemplary moment of real sincere sadness sans ambiguous crocodile tears that Johan has exhibited. If the implications of this aren't tearjerking enough, then [[http://sinemoras09.livejournal.com/467347.html this fancomic]] depicting the unseen moment should serve to rend your heart.
34* The story about Bernard, the soldier who taught Tenma how to shoot. He'd adopted a girl whose mother he'd shot, and told Tenma that she'd never smiled since that day and that she'd hate him for the rest of his life. Thanks to Tenma, she seems to recover. The last shot we see of them is the two holding hands, walking home in the rain. The girls looks up to him and smiles.
35* From episode 13: "I finally have your undivided attention".
36* When Nina was going through a major FreakOut and Dieter quickly [[GetAHoldOfYourselfMan snaps her out of it]] and starts to remind her that they should make happy memories for themselves, like Tenma told him. Dieter [[TenderTears starts crying]] in the middle of all this and... well... just the beautiful amount of emotion in his voice... just *sniff*. Doubles as a SugarWiki/{{Heartwarming Moment|s}}.
37* Wim. Just... everything regarding ''Wim.'' His mom abandoned him, his alcoholic dad beats him and makes him work for booze money, and he gets tormented constantly by the other kids for it. The old man he works for, revealed to be Franz Bonaparta, is the only person shown to be consistently nice to him. Then Johan and Roberto come to town and kickstart a massacre of everybody there, and dozens of his neighbors are murdered, his employer among them, and also Grimmer, who earlier befriended the boy. ''Then'' when Johan realizes that Tenma will not shoot him on sight, he decides to move things along by putting a gun to Wim's head and [[SadisticChoice telling Tenma that if he doesn't kill Johan, Johan will shoot Wim.]] And '''then''' Wim's father shows up, and upon seeing Johan with a gun pointed at his son, he freaks out and [[PapaWolf puts a bullet in]] [[BoomHeadshot Johan's head,]] and ends up getting arrested for it when the authorities finally show up to help. The last we see of Wim is him being pulled away from his father by policemen, crying and desperately trying to tell them that his dad was in the right. Keep in mind that he's no older than [[TagalongKid Dieter.]]
38** In the same scene, Johan's face when Nina tells she forgives him. He merely stares at her and mutters that "some things shouldn't be forgiven". Despite his actions, you come to learn that Johan isn't the actual monster of the story.
39--> '''Wim''': Dad was trying to save me! ...My dad's not a murderer!
40** What makes this even worse is that this is probably the first time the boy sees that his father actually ''did'' care for him. And not only that, nearly everyone Wim was close to and liked, is dead, so it's all the more heartbreaking for him to see his father, who finally showed concern for the boy, be taken away by police. Essentially, Wim loses ''everyone'', though there is hope his father won't be sent to prison, since he shot at Johan trying to defend Wim and didn't actually kill Johan.
41* Everything about Runge's near-nonexistent relationship with his family, especially this line concerning his daughter:
42--> '''Runge''': We lived our lives without knowing one another.
43** After he burns a chance to meet with his daughter and his newborn grandchild: "[[ToKnowHimIMustBecomeHim I am Tenma.]] I am ''alone.''"
44* "I've spoken with your mother. She loves you very much."
45** "You had a name".
46

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